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Bone matrix material containing various proteins secreted by umbilical cord mesenchymal stem cells and preparation method thereof

A technology of stem cells and matrix materials, which is applied in the field of bone matrix materials, can solve the problems of difficulty in obtaining seed cells, difficulty in large-scale preparation, and difficulty in clinical application, and achieves good osteogenic induction function, convenient storage and transportation, and strong osteogenesis. effect of inducing activity

Active Publication Date: 2014-12-17
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Problems solved by technology

[0006] Therefore, how to solve the problems of difficult acquisition, high cost, clinical application, transportation, and large-scale preparation of seed cells in bone tissue engineering research and clinical application is a concern in this field.

Method used

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  • Bone matrix material containing various proteins secreted by umbilical cord mesenchymal stem cells and preparation method thereof
  • Bone matrix material containing various proteins secreted by umbilical cord mesenchymal stem cells and preparation method thereof
  • Bone matrix material containing various proteins secreted by umbilical cord mesenchymal stem cells and preparation method thereof

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Embodiment Construction

[0058] 1. Reagents and materials: hydrochloric acid (commercially available analytically pure), DMEM / F12 culture medium (hyclone company, the United States), DBM (Beijing Daqing Biotechnology Co., Ltd., Beijing), sheep umbilical cord (the third military doctor of the Chinese People's Liberation Army University Southwest Hospital, China),

[0059] Two. The preparation method of the bone matrix material inoculated with umbilical cord mesenchymal stem cells:

[0060] 1. Decalcification

[0061] (1) Take commercially available or self-made allogeneic bone matrix materials, put 0.2-0.6M hydrochloric acid in an airtight glass container, put 5ml / g bone matrix into it for soaking, and soak for 24 hours at room temperature, every 8 Replace the hydrochloric acid every hour, the treated bone matrix is ​​spongy, can be compressed and automatically return to its original shape, put it into sterile distilled water and rinse and soak repeatedly until PH≈7;

[0062] (2) Sterilized storage ...

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Abstract

The invention relates to a bone matrix material containing various proteins secreted by umbilical cord mesenchymal stem cells and a preparation method thereof. The material is an animal bone matrix material with the umbilical cord mesenchymal stem cells inoculated on the surface of a decalcified bone matrix and used for secreting osteogenic induction function proteins to promote osteogenesis. The bone matrix material has natural structures and characteristics of bone tissues and a good osteogenic induction effect, has a better osteogenesis promoting effect in a bone defect repair process, and is helpful for large-scale preparation and clinical application of tissue-engineered bones.

Description

technical field [0001] The invention relates to a biomedical bone matrix material, in particular to a bone matrix material containing various proteins secreted by umbilical cord mesenchymal stem cells and a preparation method thereof. Background technique [0002] Bone defects caused by various reasons such as trauma, infection, and tumors are very common, and their treatment and repair are one of the most common problems in orthopedic clinics. However, the ideal method for repairing bone defects is still being explored. In recent years, individualized tissue engineered bone repair methods have brought new hope to bone defect repair. However, this repair method requires a series of tedious processes such as in vitro construction, cell expansion, and bone replantation. The cycle is as long as 3-4 weeks, the cost is expensive, the technical standards are strict, and it is affected by the quality of the patient's own seed cells, age, disease Affected by many factors, it is sti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61L27/38A61L27/36A61L27/54
Inventor 许建中侯天勇常正奇李志强罗飞吴雪晖邓墨渊王品品
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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