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Extraction method and application of safflower yellow pigment

A technology of safflower yellow pigment and extraction method, which is applied in the field of extraction of safflower yellow pigment, can solve the problems of loss of active ingredients, creation of economic benefits, low yield, etc., and achieve the effect of less purification times, low cost and simple process

Inactive Publication Date: 2014-01-01
BEIJING SUNHO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the purity of products purified by macroporous adsorption resin or membrane technology has been greatly improved, but the yield is low, and the active ingredients are seriously lost during the purification process.
For example, CN1284794C adopts the technological process of "extraction-separation-alcohol precipitation-multiple purification-preparation". The extract obtained by this method has high purity, but it needs to be purified more than twice, and the yield is less than 2%, which is difficult to create economic benefits
In CN1935176B, the technological process of "extraction-separation-purification-preparation" is also used. The content of the safflower extract obtained by this method is relatively high, but the cost of the ceramic membrane used during purification is high and the safflower yellow pigment content of the obtained extract is low. high

Method used

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  • Extraction method and application of safflower yellow pigment
  • Extraction method and application of safflower yellow pigment
  • Extraction method and application of safflower yellow pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0041] 1 kg of medicinal safflower was extracted twice with 10L of 80℃ water for one hour. The two extracts were combined and filtered to obtain filtrate I, which was separated on an HPD600 macroporous adsorption resin column (column diameter-to-height ratio 1:9), followed by washing the column with one column volume of water and seven column volumes of 30% ethanol for elution. Collect and combine the 30% ethanol eluate, filter through an ultrafiltration membrane with a molecular weight cutoff of 100,000, concentrate on the nanofiltration membrane, collect the retentate of the nanofiltration membrane, add 95% ethanol to adjust to a solution containing 80% ethanol for alcohol precipitation, After 12 hours, press filtration with a 0.45μm microporous filter membrane. The filtrate was concentrated to alcohol-free to obtain filtrate II. The pH was adjusted to 5.00 with acetic acid and separated on a polyamide column (column diameter-to-height ratio 1:8). Sequentially eluted with 2 c...

Embodiment II

[0043] 1kg of medicinal safflower was extracted with 10L of 70℃ water for 1 hour, and extracted twice. The two extracts were combined and filtered to obtain filtrate I, which was separated on an HPD600 macroporous adsorption resin column (column diameter-to-height ratio 1:9), followed by washing the column with one column volume of water and seven column volumes of 30% ethanol for elution. Collect and combine the 30% ethanol eluate, filter through an ultrafiltration membrane with a molecular weight cutoff of 100,000, concentrate on the nanofiltration membrane, collect the retentate of the nanofiltration membrane, add 95% ethanol to adjust to a solution containing 80% ethanol for alcohol precipitation, After 12 hours, filter and concentrate the filtrate until there is no alcohol to obtain filtrate II. Adjust the pH to 3.30 with hydrochloric acid, and apply a polyamide column (column diameter-to-height ratio 1:8) for separation. One column volume of 8% hydrochloric acid Elution w...

Embodiment III

[0045] 1kg of medicinal safflower was extracted twice with 10L of water at 30°C for 1 hour. The two extracts were combined and filtered to obtain filtrate I, which was separated on an HPD600 macroporous adsorption resin column (column diameter-to-height ratio 1:9), followed by washing the column with one column volume of water and seven column volumes of 30% ethanol for elution. Collect and combine the 30% ethanol eluate, filter through an ultrafiltration membrane with a molecular weight cutoff of 200,000, concentrate on the nanofiltration membrane, collect the retentate of the nanofiltration membrane, add 95% ethanol to make a solution containing 70% ethanol for alcohol precipitation, After 24 hours, press filter with 0.22μm microporous filter membrane, concentrate the filtrate to be alcohol-free to obtain filtrate II, adjust the pH to 4.50 with citric acid, and apply polyamide column (column diameter to height ratio 1:9) for separation , Followed by elution with 2 column volu...

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Abstract

The present invention relates to an extraction method and an application of a safflower yellow pigment. According to the present invention, safflower is subjected to water crude extraction, separation, filtration and ethanol precipitation, and the obtained substance is added to a polyamide column, and then is subjected to elution of impurities through acid washing, alkali solution elution and concentration drying to obtain the safflower yellow pigment, wherein the obtained safflower yellow pigment and an acceptable injection adjuvant are mixed and prepared into the injection according to the conventional injection preparation process, during polyamide column elution, an acid elution liquid is adopted to elute impurities, an alkali elution liquid is adopted to elute the safflower yellow pigment, is preferably an ethanol solution of ammonia, and the pH value of the ethanol solution is 9.50-10.50. The safflower yellow pigment prepared through the method has high safflower yellow pigment content.

Description

Technical field [0001] The invention belongs to the technical field of medical product research and development, and particularly relates to a method and application for extracting safflower yellow pigment from safflower. technical background [0002] Cardiovascular disease is the number one killer of human health. In recent years, with the aging of our country’s population and changes in people’s work, life, diet and environment, the frequency of cardiovascular and cerebrovascular diseases such as coronary heart disease has also increased year by year, and serious Threatening the health of the people. Many active substances in natural products have anti-myocardial ischemia and anti-hypoxia effects, and some of them have been developed into new drugs for the treatment of coronary heart disease and angina pectoris. Therefore, finding effective substances with anti-myocardial ischemia and anti-anoxia physiological activities from natural products is one of the effective ways to ob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/286A61P9/10A61P9/00A61P29/00A61K31/351A61K133/00
Inventor 颜克序李厚全范辉王翀李冠业冯书香郭顺
Owner BEIJING SUNHO PHARMA
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