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Acer palmatum Sangokaku tissue culture propagation process

A technology of red maple and chicken feet, which is applied in the field of tissue culture and propagation technology of red maple, can solve the problem that the method of tissue culture propagation of red maple has not been reported, and achieves low production cost, simple and convenient cultivation process, and high cultivation efficiency. high effect

Active Publication Date: 2014-01-01
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is a relatively common and precious ornamental variety in the market today, but there has never been a report on the tissue culture propagation method of red maple

Method used

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  • Acer palmatum Sangokaku tissue culture propagation process
  • Acer palmatum Sangokaku tissue culture propagation process
  • Acer palmatum Sangokaku tissue culture propagation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The tissue culture propagation technique of Acer palmatum red maple comprises the following steps:

[0023] S1. Sterilize the explants, collect disease-free branches at noon or afternoon on a sunny day, collect disease-free branches of Acer palmatum and red maple, remove excess leaves, cut into stems with buds for sterilization, and use alcohol to add alcohol for sterilization Mercury combined sterilization method, first sterilize with alcohol for 30s, then sterilize with mercuric chloride for 5min, and then wash with sterile water for 4 to 6 times;

[0024] S2, inoculation and propagation, inoculate the sterilized stem section with buds on the growth medium, the medium is MS+IAA0.5mg / L, and place the inoculated culture material in a light intensity of 2000-3000LX, The light time is 16h / d, and the temperature is 24-26°C in a cultivation room for 15 days to induce germination of axillary buds, such as figure 1 shown;

[0025] S3, subculture proliferation culture, the g...

Embodiment 2

[0028] The tissue culture propagation technique of Acer palmatum red maple comprises the following steps:

[0029] S1. Sterilize the explants. Collect disease-free branches of Acer palmatum and red maple in the same year at noon or afternoon on a sunny day, remove excess leaves, cut into stems with buds and sterilize them. Sterilization method, first sterilize with alcohol for 50 seconds, then sterilize with mercuric chloride for 4 minutes, and then wash with sterile water for 4 to 6 times;

[0030] S2, inoculation and propagation, inoculate the sterilized stem section with buds on the growth medium, the medium is MS+IAA0.5mg / L, and place the inoculated culture material in a light intensity of 2000-3000LX, The light time is 16h / d, and the temperature is 24-26°C in a culture room for 3 weeks to induce germination of axillary buds, such as figure 1 shown;

[0031] S3, subculture proliferation culture, the germinated axillary buds are cut off, inoculated on the subculture mediu...

Embodiment 3

[0034] The tissue culture propagation technique of Acer palmatum red maple comprises the following steps:

[0035] S1. Sterilize the explants. Collect disease-free branches of Acer palmatum and red maple in the same year at noon or afternoon on a sunny day, remove excess leaves, cut into stems with buds and sterilize them. Sterilization method, first sterilize with alcohol for 60 seconds, then sterilize with mercuric chloride for 3 minutes, and then wash with sterile water for 4 to 6 times;

[0036] S2, inoculation and propagation, inoculate the sterilized stem section with buds on the growth medium, the medium is MS+IAA0.5mg / L, and place the inoculated culture material in a light intensity of 2000-3000LX, The light time is 16h / d, and the temperature is 24-26°C in a culture room for 2 weeks to induce germination of axillary buds, such as figure 1 shown;

[0037] S3, subculture proliferation culture, the germinated axillary buds are cut off, inoculated on the subculture mediu...

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Abstract

The invention discloses an acer palmatum Sangokaku tissue culture propagation process which comprises the following steps: S1, explant sterilization; S2, inoculation and propagation, namely inoculating a sterilized stem section with a bud to a culture medium for growth, culturing for 2-3 weeks, and inducing the germination of an axillary bud; S3, enrichment subculture, namely shearing the germinated axillary bud, inoculating on a subculture medium, culturing for 3-5 weeks, and inducing the generation of caespitose buds; S4 rooting culture, namely cutting adventitious buds positioned on the caespitose buds, inoculating on a rooting culture medium, culturing for 2-3 weeks, and inducing the generation of a root. The acer palmatum Sangokaku tissue culture propagation process disclosed by the invention can be used for building a Sangokaku tissue culture propagation system, and realizing the breakthrough of the Sangokaku tissue culture propagation system, is low in production cost, high in propagation coefficient, short in propagation period, an effective way for the fast propagation of acer palmatum Sangokaku, and capable of preventing the virus accumulation, and achieving the easiness for domesticated seedling survival, and by using the process, the issue culture seedlings with consistent inheritable characters and the sterile acer palmatum Sangokaku seedlings can be relatively easily obtained.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to the tissue culture propagation technique of Acer palmatum and red maple. Background technique [0002] The genus Acer (Aceraceae) is usually referred to as maple, and it is also called "maple" or "maple" in habit. There are more than 200 kinds of maple trees in the world, distributed in Asia, Europe, North America and northern Africa. There are more than 160 species in my country, accounting for more than 75% of the world's total. They are distributed all over the country, and are mainly produced in the Yangtze River Basin and the provinces to the south. Maple trees can be used as building materials, paper-making raw materials, medicine extraction, industrial oil and nectar plants. Some maple trees are rich in tree shape, elegant in posture, rich in leaf shape and changeable in leaf color, and are known as world-famous foliage plants. Acer palmatum (Acer palmatum)...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 马建华王小辉朱晓菲吴佳川沈香兰
Owner SICHUAN COLORLINK CO LTD
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