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Klebsiella oxytoca and application thereof in allitol production

A technology of Klebsiella and alliitol, applied in the field of microorganisms, can solve problems such as poor bacterial substrate specificity, and achieve the effect of broad application prospects

Active Publication Date: 2013-12-25
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent document discloses a method for producing a variety of rare sugars and sugar alcohols by using the oxidation and reduction of Enterobacter agglomerans221e, but the substrate specificity of the bacteria is poor, such as D-fructose and D-psicose Conversion of the mixture may generate other by-products

Method used

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  • Klebsiella oxytoca and application thereof in allitol production
  • Klebsiella oxytoca and application thereof in allitol production
  • Klebsiella oxytoca and application thereof in allitol production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Screening and preservation of Klebsiella oxytoca G4A4CGMCC No.7662

[0028] 1. Screening of Klebsiella oxytoca G4A4 strains

[0029] A gram-negative bacillus strain that can utilize D-psicose was isolated from a soil sample in Nanning, Guangxi. concentration) D-psicose inorganic salt medium (formulation: 0.25% (NH 4 ) 2 S0 4 , 0.25% KH 2 P0 4 , 0.5% K 2 HP0 4 , 0.01% MgS0 4 ·7H 2 0, 0.05% yeast powder, according to the mass / volume percentage concentration g / 100ml, the balance is water, pH7.0), a strain of bacteria was obtained by culturing at 37°C, and its 16s rRNA gene sequence is as shown in the sequence list 1, which was identified as Klebsiella oxytoca by analysis and named G4A4.

[0030] 2. Morphological characteristics, culture conditions and biochemical characteristics of Klebsiella oxytoca G4A4 strain

[0031] 1. Morphological characteristics of Klebsiella oxytoca G4A4

[0032] Straight rod shape, the length of the bacteria is about 0.6-2....

Embodiment 2

[0042] Example 2. Production of allitol by using Klebsiella oxytoca (Klebsiella oxytoca) G4A4CGMCC No.7662 strain (small-scale production)

[0043] 1. Cultivation of Klebsiella oxytoca G4A4

[0044] Inoculate Klebsiella oxytoca G4A4CGMCC No.7662 in LB modified medium (each 1L medium contains: 0.2-0.5g peptone, 0.2-0.5g yeast powder, 0.2-0.5g sodium chloride. ) and other low-sugar and high-nitrogen medium, under the conditions of 37°C, pH7.0, 150-250rpm (preferably 200rpm), the strains were fermented to OD 600 When the value is 3.0-4.0, the strain enters the stationary phase of growth.

[0045] 2. Domestication of Klebsiella oxytoca G4A4

[0046] Klebsiella oxytoca (Klebsiella oxytoca) G4A4CGMCC No.7662 was inoculated at an inoculum size of 1% in an inorganic salt medium containing 1% (mass / volume concentration, g / 100ml) xylitol (the formula is : Concentration by mass / volume percentage, g / 100ml, 0.25% (NH 4 ) 2 S0 4 , 0.25% KH 2 P0 4 , 0.5% K 2 HP0 4 , 0.01% MgS0 4 ·...

Embodiment 3

[0051] Example 3. Production of allitol by Klebsiella oxytoca G4A4CGMCC No.7662 strain (small-scale production)

[0052] The method is the same as in Example 2, except that the temperature and pH of the resting cell transformation reaction are increased to 45°C and 9.0 respectively, and the cell concentration is increased to OD 600 =40. Under these conditions, the reaction reached equilibrium after 36 hours, and an allitol reaction solution was obtained. The conversion rate of allicitol in this example is 76.8%. After increasing the cell concentration, the conversion rate has been significantly improved compared with Example 2.

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Abstract

The invention discloses a new stain of Klebsiella oxytoca G4A4 CGMCC No.7662 and application thereof in allitol production, that is the screening bacterial stain of Klebsiella oxytoca G4A4 is prepared into resting cell and is used for allitol production, thereby compensating for the insufficiency of the prior art. The bacterial stain of Klebsiella oxytoca G4A4 has ribose ribitol dehydrogenase, which has a wider substrate spectrum, and under the anaerobic condition, D-psicose can be reduced to allitol. The bacterial stain of Klebsiella oxytoca G4A4 can be applied to the allitol production with D-psicose as substrate, and simple medium can be used for rapid growth and large scale enlarging culture, and the developed allitol production function of bacterial stain of Klebsiella oxytoca G4A4 has a wide application prospect.

Description

technical field [0001] The invention relates to a strain of Klebsiella oxytocin in the field of microorganisms and its application in producing allicitol. Background technique [0002] Rare sugar (Rare Sugar) is a kind of monosaccharide and its derivatives that exist in nature but are very low in content (defined by the International Rare Sugar Society ISRS in 2002). Its taste is similar to sucrose, but it has low calories, high stability, sweet It has the advantages of harmonious taste, no hygroscopicity, no cariogenicity, and high tolerance, which plays an important role in improving the diet of special populations. In addition, a large number of research results also show that rare sugars play an important physiological role in many aspects such as anti-cancer, scavenging free radicals, neuroprotection, etc., and can also undergo Maillard reactions with active substances such as proteins and peptides to optimize their functional activity ( Zeng Y, Zhang X, Guan Y, Sun Y....

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/02A61K31/7004A61P3/10A23L1/29C12R1/22A23L33/00
Inventor 孙媛霞朱玥明韩文佳杨建刚马延和
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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