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Short peptide for bacterium resisting, itching relieving, inflammation diminishing, swelling eliminating and scar removing, and its application

A short peptide and scar technology, applied in the field of biotechnology and medicine, can solve the problems of skin diseases that cannot be cured, have side effects, and poor curative effect

Active Publication Date: 2015-05-20
福建蓝昊肽生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are various ointment drugs on the market to treat skin diseases, but some of these ointment drugs have poor curative effect and some have side effects and cannot cure skin diseases. The reason is that most of these drugs are inorganic compounds, the products of chemical synthesis
However, the use of bioactive peptides—short peptides formed from the amino acid sequences of protein fragments—as skin antibacterial, antipruritic, antiinflammatory, swelling, and scar removal drugs has not been reported in the literature so far.

Method used

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  • Short peptide for bacterium resisting, itching relieving, inflammation diminishing, swelling eliminating and scar removing, and its application
  • Short peptide for bacterium resisting, itching relieving, inflammation diminishing, swelling eliminating and scar removing, and its application
  • Short peptide for bacterium resisting, itching relieving, inflammation diminishing, swelling eliminating and scar removing, and its application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the side chain protection at the same time.

[0028] The amino acid sequence of the short peptide, Tyr-Leu-Leu-Glu-Ala-Lys or Tyr-Leu-Tyr-Glu-Val-Ala-Arg or Tyr-Leu-Tyr-Glu-Val-Ile-Arg as the main sequence, from Short peptide fragments, short peptide analogs, short peptide derivatives, short peptide variants, etc. that are truncated at any position into 3 or 4 or 5 or 6 amino acids and have the same biological activity as the short peptide have the same biological activity as the short peptide. Efficacy as described in the invention.

[0029] Nucleotides are prepared by...

Embodiment 1

[0049] Antibacterial experimental effect (synthesis of the short peptide involved in the present invention: Tyr-Leu-Tyr-Glu-Val-Ala-Arg)

[0050] The short peptide of the present invention is chemically synthesized by solid-phase Fmoc method. After HPLC purification, the purity exceeds 97%. Staphylococcus aureus is used for antibacterial experiments. That is, first inoculate and activate the slant of the bacteria, place it in a 37°C incubator for 24 hours, and observe the growth of pure Staphylococcus aureus on the slant the next day; for the activated Staphylococcus aureus, take a small amount and 100ml of liquid medium (prepared in 500ml system) , beef extract 2.5g, peptone 7.5g and NaCl 2.5g) in a shaker (37°C, 150r / min) for 12h, after which it can be seen that the culture solution changes from clarification to turbidity. The short peptide involved in the present invention is made into a liquid with a concentration of 0.1 mg / ml, and 100 ml is added to a solid medium (prepar...

Embodiment 2

[0052] Neurodermatitis (synthesis of the short peptide involved in the present invention: Tyr-Leu-Leu-Glu-Ala-Lys)

[0053] The short peptide of the present invention is chemically synthesized by solid-phase Fmoc method. After HPLC purification, the purity exceeds 97%. Staphylococcus aureus is used for antibacterial experiments. That is, first inoculate and activate the slant of the bacteria, place it in a 37°C incubator for 24 hours, and observe the growth of pure Staphylococcus aureus on the slant the next day; for the activated Staphylococcus aureus, take a small amount and 100ml of liquid medium (prepared in 500ml system) , beef extract 2.5g, peptone 7.5g and NaCl 2.5g) in a shaker (37°C, 150r / min) for 12h, after which it can be seen that the culture solution changes from clarification to turbidity. The short peptide involved in the present invention is made into a liquid with a concentration of 0.1 mg / ml, and 100 ml is added to a solid medium (prepared in a 500 ml sys...

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Abstract

The invention provides a short peptide for antibacterial, antipruritic, anti-inflammatory, detumescence, and scar removal and application thereof. The amino acid main sequence of the short peptide fragments, analogs, derivatives, and variants of the short peptide has ≥70% identity and ≥90% similarity with the short peptide amino acid main sequence; short peptides, nucleotides, short Peptide fragments, short peptide analogs, short peptide derivatives and short peptide variants are used for the preparation of antibacterial, antipruritic, anti-inflammatory and detumescent drugs for various skin disinfection or treatment of skin diseases, for the preparation of neurodermatitis, contact dermatitis, chronic eczema , Drug application for topical treatment of insect bite dermatitis and pruritic skin diseases. The short peptide involved in the invention has a clear molecular structure, mature production technology, and the finished product is non-toxic, harmless, and has no side effects. Its remarkable feature is that it does not leave scars after being cured.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a short peptide for antibacterial, antipruritic, anti-inflammatory, detumescence, and scar removal and its application. Background technique [0002] All kinds of skin redness, inflammation, and itching are common multiple skin diseases that seriously affect people's health. They are usually divided into neurodermatitis, contact dermatitis, acute and chronic eczema, insect bite dermatitis, and itchy skin diseases. There are various ointment drugs on the market to treat skin diseases, but some of these ointment drugs have poor curative effect and some have side effects and cannot cure skin diseases. The reason is that most of these drugs are inorganic compounds, the products of chemical synthesis. However, the use of bioactive peptides—short peptides formed from the amino acid sequences of protein fragments—as skin antibacterial, antipruritic, anti-inflam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12N15/11A61K38/08A61K48/00A61P17/02A61P17/00A61P17/04A61P15/00A61P31/04A61P29/00
Inventor 李昊
Owner 福建蓝昊肽生物科技发展有限公司
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