Walnut green seedcase extract compound preservative as well as preparation method and application thereof
A technology of walnut green skin and compound fresh-keeping, which is applied in the application, fruit and vegetable fresh-keeping, food preservation and other directions, can solve the problems of waste of resources, no large-scale promotion and application, pollution of the environment, etc., to prevent water loss, inhibit the reproduction of microorganisms, The effect of prolonging the storage time
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Embodiment 1
[0031] The comparative test of the antibacterial effect of walnut green husk extract and walnut green husk extract composite preservative is as follows (using the growth rate method):
[0032] Tested species: Penicillium citrinu, Rhizopus stolonifer, Botryis Cinerea, Fusarium oxysporum and Alternaria;
[0033] PDA potato medium is used, which meets the GB4789.28-94 standard.
[0034] Preparation of walnut green peel extract: Take walnut green peel dry powder according to 1g: (6-10) mL, use 50% to 80% ethanol aqueous solution as extractant, soak and extract twice at room temperature, each time for 1 hour, and combine the extracts, Then the extract was rotovapped and redissolved in distilled water for later use (the ratio of solid to liquid was 1:4 to 1:8, respectively).
[0035] Preparation of composite preservative: Dissolve the above-mentioned walnut green husk sample with chitosan solution and emulsifier according to the following formula: walnut green husk extract concentr...
Embodiment 2
[0052] The present embodiment provides a composite antistaling agent of walnut green husk extract. In terms of weight percentage, the composite antistaling agent of walnut green husk extract contains: 3% of walnut green husk extract, 1% of chitosan, and 1% of Tween 80. %, distilled water is 95%.
[0053] The above-mentioned walnut green husk extract composite preservative is prepared through the following steps:
[0054] 1. Preparation of walnut green peel extract in all-natural plant compound preservative
[0055] Peel off the green skin of fresh walnuts, pulverize them at -30°C to -40°C, and freeze-dry them under a vacuum of 18Pa at a temperature of -50°C to obtain dry walnut green skin powder;
[0056] Take 150g dry walnut green peel powder and pass through a 40-mesh sieve, add extractant (80% ethanol solution) 2000mL, soak for 12 to 24 hours, and obtain a solid-liquid mixture;
[0057] Suction filter the solid-liquid mixture under 40kPa vacuum condition, take the filtrat...
Embodiment 3
[0063] The present embodiment provides a composite antistaling agent of walnut green husk extract. In terms of weight percentage, the composite antistaling agent of walnut green husk extract contains: 4% of walnut green husk extract, 1% of chitosan, and 1% of Tween 80. %, distilled water is 94%.
[0064] The above-mentioned walnut green husk extract composite preservative is prepared through the following steps:
[0065] 1. The preparation method of the walnut green peel extract in the composite preservative is the same as in Example 2.
[0066] 2. Preparation of walnut green peel extract compound preservative
[0067] Dissolve the walnut green husk extract and chitosan in distilled water according to the following weight ratio, and continue to stir for 30 minutes at a speed of 300rpm / min, and stir until uniform, then add the emulsifier Tween 80, and disperse it ultrasonically at a frequency of 40kHz 40min to obtain a uniform walnut green husk extract composite preservative....
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