A kind of one-step seedling growth medium and method for in vitro culture of unique flavor
A technology of in vitro cultivation and cultivation methods, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., to achieve the effects of high reproduction coefficient and regeneration frequency, simple cultivation procedures, and short regeneration cycle
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Embodiment 2
[0038] In order to avoid the error of the experimental results caused by human factors, the applicant asked different researchers to inoculate 6 per bottle under the same conditions, and inoculate 8 bottles in total, using the unique one-step seedling growth medium identical to the embodiment, The same experimental operation as in Example 1 was carried out, and the experimental results are shown in Table 5 and Table 6.
[0039] Table 5 Effects of different hormone combinations on leaf callus induction
[0040]
[0041]
[0042] Table 6 Effects of different hormone combinations on the differentiation of adventitious buds from leaves
[0043]
[0044] Table 7 Survival rate of test-tube plantlets hardened by different hormone combinations
[0045]
[0046]
[0047]Analysis of the experimental data obtained in Table 5 shows that, except for the control group, the one-step seedling growth medium of each group can better induce the leaves of the unique herb, and its ...
Embodiment 3
[0053] A method for one-step seedling growth by in vitro culture of test-tube plantlet leaves comprising the following steps:
[0054] (1) Prepare the unique one-step seedling growth medium: add 2.0mg of 6-benzylaminopurine and 1.0mg of naphthaleneacetic acid to 1LMS medium, sterilize and set aside;
[0055] (2) Select the unique aseptic test-tube plantlets from the same source, and under aseptic conditions, cut out the fully expanded leaves at the top of about 0.5cm×0.5cm, and stick the back of the leaves on the medium described in step 1;
[0056] (3) Place the post-inoculation culture medium obtained in step (2) in an incubator at 20°C, and culture in complete darkness. After 7-10 days, carry out 16h / d light culture, with a light intensity of 1200Lx;
[0057] (4) After 55 days of light culture in the primary culture medium, regenerated plants of Duyiwei were obtained.
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