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Microbial preparation for treating tobacco stems and preparation method thereof

A microbial preparation and microbial technology, applied in the field of tobacco additives, can solve unseen problems and achieve the effects of simple operation, convenient large-scale industrial production, and strong controllability

Active Publication Date: 2013-12-18
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is no report on microbial preparations prepared by the microbial strain Aureobasidium pullulans OF-01 strain (CGMCC No.7609)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The strain source of microorganism Aureobasidium OF-01 of the present invention:

[0032] The invention provides a microbial preparation for improving the quality of tobacco stems. The preparation of the microorganism is obtained by the following method: pulverize a sample of Honghua Dajinyuan tobacco leaves aged in storage for 3 years, and take 5 grams of pulverized tobacco powder Put it into a triangular flask with 100ml of sterile water, shake it on a shaker at 100-250rpm for 30 minutes, filter to remove the smoke dust, and apply the suspension to MYP medium (malt extract 7g, yeast extract 0.5g, peptone 2.5 g, agar 20g, water 1L, pH7.0) plate, 200 microliters / dish, a total of 50 dishes, cultured in an incubator at 28°C for 5-7 days to purify a single microorganism with MYP medium, from the surface of the purified tobacco leaves The OF-01 strain with aroma-producing properties was screened from microorganisms, which was identified as Aureobasidium pullulans.

[0033]...

Embodiment 2

[0037] Microorganism preparation of the present invention adopts following steps to prepare:

[0038] 1. The liquid strain of the mold OF-01 isolated from the flue-cured tobacco leaves in Example 1 was cultured on a test tube slant: inoculate the thalli of the bacterial strain OF-01 into the MYP medium (7 g of malt extract, 0.5 g of yeast extract , peptone 2.5g, agar 20g, water 1L, pH7.0) on a slant, cultured at 20-30°C for 4-10 days to obtain seeds cultured on the slant, the suitable culture condition is 26-32°C, the best is at Cultured at 28°C for 7 days.

[0039] 2. Liquid seed culture:

[0040] Seeds cultivated on slant planes are inoculated into MYP liquid medium in Erlenmeyer flasks and cultured on a shaker at 15-30° C. and 50-300 rpm for 3-7 days. The optimum culture condition is 28°C, 200rpm for 4 days.

[0041] 3. Mass cultivation in fermenter:

[0042] Put the seeds after liquid seed culture into the MYP liquid medium in the fermenter at a ratio of 1-5% (V / V), an...

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PUM

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Abstract

The invention discloses a microbial preparation for tobaccos and a preparation method and application thereof, belonging to the technical field of tobacco additives. According to the preparation method, the corresponding microbial preparation is obtained through carrying out liquid fermentation culture on producing strains, wherein the producing strains are aureobasidium pullulans OF-01 strains (CGMCC (China General Microbiological Culture Collection Center) No.7609). The microbial preparation disclosed by the invention has the advantages that the characters, such as aroma amount, pleasantness and the like, of cut stems can be improved obviously, offensive odors, irritation and the like are reduced, the concentration and momentum of smoke are reduced, and the aftertaste becomes comfortable, so that the blended amount of the cut stems in cigarettes is further increased, and the aims of reducing harm, lowering tar content and reducing the cigarette production cost are achieved.

Description

technical field [0001] The invention belongs to the technical field of tobacco additives, more specifically, the invention relates to a microbial preparation which can improve the quality of cigarettes, a preparation method of the microbial preparation and its application in tobacco stem treatment and cigarette processing. Background technique [0002] Tobacco stems are leftovers with low utilization rate produced in cigarette production, and their chemical composition is almost the same as that of tobacco leaves. In the modern cigarette production process, tobacco stems are processed and added to cigarettes in a small proportion, which can not only save costs, but also reduce the amount of tar in cigarettes. [0003] However, due to the obvious burning sensation and heavy woodiness of the tobacco stems, the proportion of tobacco stems in the cigarette formula is relatively low, and they are heavily dependent on tobacco leaves. Therefore how to improve the quality of tobacc...

Claims

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Application Information

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IPC IPC(8): C12N1/14A24B15/20C12R1/645
Inventor 杨蕾杨乾栩蔡波何雪峰党立志杨清段焰青杨洪明周国福李勇师建全冯洪涛莫明和
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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