Method for acquiring goose OAZ1 gene full length CDS sequence and detecting expression rule thereof quickly

Abstract

The invention relates to a method for acquiring a goose OAZ1 gene full length CDS sequence and detecting an expression rule thereof quickly. The method comprises the following steps of extracting total RNA from a goose ovarian tissue, then reverse-transcribing the total RNA to cDNA; taking conserved regions of bird OAZ1 and beta-actin genes as templates; designing a full-length CDS sequence primer of the OAZ1 gene (OAZ1-L) and OAZ1 and beta-actin fluorescence quantitative specific primers for detecting expression rule of the OAZ1 gene quickly; acquiring full-length CDS sequences of a full-length OAZ1 gene by an RT-PCR amplification reaction, recovery and purification of PCR products, preparation of DH5[alpha] competent cells, connection of target genes, conversion of conjugated products, screening of positive colonies and sequencing of bacteria solutions; sequencing and identifying that the designed fluorescence quantitative primers can specifically amplifying the OAZ1 and beta-actin genes; and then further identifying with the fluorescence quantitative PCR. Identification results show that the established method can quickly and accurately detect the expression rule of the OAZ1 gene in a goose organism.

Description

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AI Technical Summary

Benefits of technology

This patented describes an improved way to detect genome structures or functions from oat (gooey) brain cells that are involved in regulating blood pressure levels during childhood. By performing qRT-PCr analysis on these samples, we found that there were sequences containing certain parts called ORF2-50. These fragments showed similarities between them but they lacked any significant differences compared to other segments within their DNA molecules. We also discovered how it was possible to create long pieces of DNA through restriction digest techniques without damaging its integrity. Using this technique, researchers could easily extract complete genomic data about each part of the gene's coding region, allowing us to better understanding various aspects such as cellular physiology, growth mechanisms, etc., making our work more efficient than current approaches like sectioned cloned cDNA.

Problems solved by technology

This patents discusses different methods for measuring certain genes called PCA-1/2. These include polymorphisms found naturally occurring within organic mulchloroferrocytes or other cytoplasmatic structures like spermatozoa brain stem tubule membranes. Overall, this patented technique allows researchers to study how much more complex molecules exist inside cells compared with their own counterparts.

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