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Fermental cultivation method for marine pseudoalteromonas

A technology of Pseudomonas alternadomonas and Alteromonas bacterium, which is applied to the fermentation culture of fish bacteria vaccine Pseudomonas marine alternatus after optimizing the fermentation conditions, and in the field of fermentation and cultivation of Pseudomonas marine alternatus, which can solve the problem of culture speed Slow, unable to adapt to large-scale industrial production and other problems, to achieve the effect of increasing the concentration of bacterial liquid

Inactive Publication Date: 2013-11-27
SHANDONG ENKANG PHARMA +1
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Problems solved by technology

However, the above-mentioned culture conditions have been unable to meet the needs of large-scale industrial production due to the slow culture speed.

Method used

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  • Fermental cultivation method for marine pseudoalteromonas
  • Fermental cultivation method for marine pseudoalteromonas

Examples

Experimental program
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Effect test

Embodiment 8

[0034] The Pseudoalteromonas haloplanktis described in Example 8 was purchased from the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures, and the strain preservation number is: 1.10250;

Embodiment 9

[0035] The Pseudoalteromonas marina described in Example 9 was purchased from the General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microorganisms, and the culture preservation number is: 1.10151.

[0036] 1. Analysis of salt concentration on culture results

Embodiment 1

[0038] A method for fermenting and cultivating marine bacteria Pseudoalteromonas, the steps are as follows:

[0039] Take the activated marine bacteria Pseudoalteromonas sp., and inoculate it in a low-salt fermentation medium at 1.5% by weight, and ferment at a fermentation temperature of 29°C, a rotational speed of 180r / min, and a liquid volume percentage of 20%. Cultivate for 18 hours, then;

[0040] The components per liter of the above-mentioned low-salt fermentation medium are as follows:

[0041] Peptone 12g, yeast extract powder 0.8g, NaCl concentration 5g, sucrose 17.5g, FeCl 3 0.015g, dilute to 1L with water, pH7.5.

[0042] After testing, the OD value of the bacterial solution was 0.75, and the concentration of the bacterial solution was 2.8×10 6 pieces / ml.

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Abstract

The invention relates to a fermental cultivation method for a marine bacterium pseudoalteromonas. The method comprises steps of inoculating the activated marine pseudoalteromonas to a low-salt fermentation medium according to a weight ratio of 1.0%-5.0%; and performing fermental cultivation under conditions comprising a fermentation temperature of 20-40 DEG C, a rotating speed of 120-260 r / min and a liquid-filling volume percentage of 10%-30% for 15-28 h. The invention also relates to a special fermentation medium for the marine bacterium pseudoalteromonas. Through optimized fermentation conditions and the medium, the marine pseudoalteromonas can grow well, the average bacteria concentration reaches 4.2*10<11> bacteria per mL, and the bacteria concentration is increased by 1-3 orders of magnitudes compared to a seawater fermentation medium and fermentation conditions at the present.

Description

technical field [0001] The invention relates to a method for fermenting and cultivating Pseudomonas marine alternosa, in particular to a method for fermenting and cultivating Pseudomonas alternosa marine bacteria vaccine after optimizing fermentation conditions, and belongs to the technical field of microbial fermentation. Background technique [0002] As early as 1942, Duff succeeded in oral immunization of steelhead trout with inactivated Aeromonas salmonicida for the first time. Although the experiment could not be repeated by later generations, it has created a new era of vaccine application in fish after all. Since then, many people have exploratory research and prepared vaccines to prevent fish diseases that are explosive and difficult to control with drugs. Among them, vibrio disease vaccines and enteric red mouth disease (ERM) vaccines have been successful. In 1975, American Vaccines Limited (AVL) began producing commercial fish vaccines. In February 1984, the fir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 陈抗王君范延辉姚志刚付荣强王惠
Owner SHANDONG ENKANG PHARMA
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