Method for identifying purity of corn seeds

Abstract

The invention discloses a method for identifying the purity of corn seeds. Compared with the prior art, by adjusting the concentrations of acrylamide and N,N-methylene bisacrylamide (MBA) in the method, for the method, the toughness and the rigidity of separation gel sheets are greatly improved; stacking gel is omitted, electrophoretic velocity is slowed down by reducing the voltage, and proteins with different molecular weights can be gathered on the same horizontal line before entering separation gel; methyl green AR-0721 is screened out in the method, and the migration rate of the methyl green AR-0721 is synchronous with the migration rate of protein molecules, thus being convenient to check whether the test procedure is finished; the sample extracting temperature and dyeing conditions are improved, the extraction and identification time is greatly shortened, the identification efficiency is improved, and the fixed procedure of the separation gel is simplified at the same time; the cleaning and storing procedures of the separation gel sheets are added, a cleaning solution and a storage solution are developed, and both the test result identification and the separation gel sheet storage are convenient.

Description

technical field [0001] The invention relates to a method for identifying the purity of corn seeds, which is specifically applied to the fields of quick identification of seed purity and identification of germplasm resources. Background technique [0002] Variety purity is the main quality index of corn seeds, which has a direct and significant impact on the quality of corn and product quality. The loss of maize production in my country due to low seed purity is staggering, and the reduction in yield due to low purity alone may offset the potential for a new variety to increase yield. [0003] Maize seed purity identification technology is one of the key points and difficulties in my country's current seed inspection work, and it is also an urgent problem to be solved in seed management work. At present, the methods for identifying the purity of seeds mainly include: morphological identification, protein electrophoresis, and molecular marker identification based on DNA molec...

AI Technical Summary

Problems solved by technology

However, the traditional electrophoresis technology has the following problems in the operation process: time-consuming protein extraction, insufficient toughness and hardness of the film, cumbersome gel preparation procedures, and low dyeing efficiency, which reduce the efficiency of the test.

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