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Extraction method for resveratrol from giant knotweed rhizome

A technology of resveratrol and Polygonum cuspidatum, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of large solvent loss, large production consumption, and many equipment, and achieve the effect of improving yield

Active Publication Date: 2013-11-20
陕西绿清生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These existing technologies all have common shortcomings such as long working hours, more equipment used, large solvent loss, low yield, large production consumption, and high cost; Sufficiently, the loss of low yield caused by the accelerated decomposition of the enzyme preparation remaining in the raw material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Get 1 kilogram of knotweed raw material (exclude moisture content) that detects resveratrol content after enzymolysis is 1.4%, add 1 gram of superoxide dismutase and add 5 liters of 80% concentration ethanol solvents simultaneously, heating temperature is controlled at Reflux extraction at 50°C for 2 hours, filter out the extract, then add 5 liters of ethanol to extract for 2 hours, so 4 times, combine the extracts obtained from each batch and control the temperature at 60°C for vacuum concentration to recover ethanol, concentrated Add the chitosan saturated solution to the extract in proportion to 5% by weight of the raw material, let it stand for 10 hours to precipitate at normal temperature, and filter after the precipitation is complete;

[0022] Intercept the solid precipitated part and add ethanol solvent with a mass concentration 20 times that of the precipitate and 40% to dissolve the obtained precipitate repeatedly and filter, discard the insoluble matter, and c...

Embodiment 2

[0026] Get 10 kilograms of Polygonum cuspidatum raw materials (exclude moisture content) and add 10 grams of superoxide dismutase and add 50 liters of 80% concentration ethanol solvents at the same time after getting the detected resveratrol content of detecting resveratrol content as 1.4%, heating temperature is controlled at 50 ℃ and reflux extracts 2 hour, after filtering out the extract, add 5 liters of ethanol to extract for 2 hours, so 4 times, the extracts obtained by each batch are combined and controlled at 60°C for vacuum concentration and recovery of ethanol. The concentrated extract is based on the weight of raw materials Add 5% chitosan saturated solution, let it stand for 12 hours to precipitate at normal temperature, and filter after the precipitation is complete.

[0027] Intercept the solid precipitated part and add ethanol solvent with a mass concentration 20 times that of the precipitate and 40% to dissolve the obtained precipitate repeatedly and filter, disc...

Embodiment 3

[0031] Get 100 kilograms of Polygonum cuspidatum raw materials (exclude moisture content) that detect resveratrol content after getting fermentation and be 1.4%, add 100 grams of superoxide dismutases and add 500 liters of 80% concentration ethanol solvents at the same time, heating temperature is controlled at 50 ℃ and reflux extracts 2 hour, after filtering out the extract, add 5 liters of ethanol to extract for 2 hours, so 4 times, the extracts obtained by each batch are combined and controlled at 60°C for vacuum concentration and recovery of ethanol. The concentrated extract is based on the weight of raw materials Add 5% chitosan saturated solution, let it stand for 12 hours to precipitate at normal temperature, and filter after the precipitation is complete.

[0032] Intercept the solid precipitated part and add ethanol solvent with a mass concentration 20 times that of the precipitate and 40% to dissolve the obtained precipitate repeatedly and filter, discard the insolubl...

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PUM

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Abstract

The invention provides an extraction method for resveratrol from giant knotweed rhizome. Plants like giant knotweed rhizome, peanut rhizome, branches and leaves of grape and mulberry leaves having undergone enzymatic hydrolysis and fermentation can be used as raw materials. The extraction method comprises the following steps: adding superoxide dismutase according to the proportion of the raw materials and then adding an ethanol solvent with a concentration of 80% for extraction; combining obtained extracts, carrying out vacuum concentration and complete recovery of ethanol, adding a saturated chitosan solution into an obtained concentrate, and allowing an extract to be precipitated in a deposition manner; adding an ethanol solvent weighing 20 times of an obtained precipitate and having a concentration of 40% to dissolve the precipitate, then carrying out filtering, concentrating a filtrate, adding a chitosan solution into the concentrated filtrate and carrying out standing and centrifugation at normal temperature so as to obtain a solid precipitate; and adding an ethanol solvent weighing 20 times of the solid precipitate and having a concentration of 40% to dissolve the solid precipitate, then carrying out filtering, concentrating a filtrate, adding a chitosan solution into the concentrated filtrate, carrying out standing and centrifugation at normal temperature so as to obtain a solid precipitate and washing the solid precipitate with purified water until a washing liquid does not contain a pigment; and successively carrying out vacuum drying and crushing so as to obtain a product with purity of 50 to 60%. Compared with a conventional production method, the extraction method provided by the invention enables yield of resveratrol to be increased by more than 30%.

Description

1. Technical field [0001] The invention relates to a method for extracting resveratrol from Polygonum cuspidatum. It is also suitable for extracting resveratrol from peanut rhizomes, grape branches and leaves, mulberry leaves and other plants as raw materials. Belongs to the field of biotechnology. 2. Background technology [0002] Resveratrol is a natural antioxidant that can reduce blood viscosity, inhibit abnormal platelet aggregation, prevent heart attack, cerebral embolism, and liver damage, and protect ischemic heart and arteries. Diastole keeps blood flowing and improves microcirculation, prevents the occurrence and development of cancer, has anti-atherosclerosis and coronary heart disease, ischemic heart disease, Alzheimer's disease, viral hepatitis, gastric ulcer and improves immunity in wound treatment Systemic activity, and anti-tumor effect. [0003] At present, the preparation of resveratrol is divided into five categories: chemical synthesis, plant cell cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C39/21C07C37/68C07C37/88
Inventor 王喜军
Owner 陕西绿清生物工程有限公司
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