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Method for intermittent co-substrate-controlled co-metabolism decomposition of difficultly degraded phenol pollutants

A co-substrate and pollutant technology, applied in the field of water environmental pollution control, can solve the problems of phenolic pollutants existing, difficult to remove, and unreasonable co-substrate dosing methods, etc.

Active Publication Date: 2013-11-20
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to provide a method for intermittent co-substrate regulation and co-metabolism to decompose refractory phenolic pollutants, so as to solve the problem that refractory phenolic pollutants exist in water for a long time and are difficult to remove in the natural state
[0006] In order to quickly realize the conversion and degradation of phenolic pollutants in surface water or sewage, improve the metabolic activity of bacteria, and avoid the problem of unreasonable and uneconomical co-substrate dosing, the present invention provides an intermittent co-substrate regulation The method of co-metabolism to decompose refractory phenolic pollutants, using easily degradable organic matter to induce microorganisms to secrete key enzymes and obtain energy to drive the initial conversion of refractory phenolic pollutants, using the co-metabolism process to degrade the co-substrate and refractory phenolic pollutants The reaction kinetics series and rate will change with the change of its concentration and properties. The co-substrate concentration is used to intermittently add the co-substrate solution to the water containing phenolic pollutants, and the dosage of the co-substrate It will also decrease gradually with the decrease of the concentration of phenolic pollutants

Method used

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  • Method for intermittent co-substrate-controlled co-metabolism decomposition of difficultly degraded phenol pollutants
  • Method for intermittent co-substrate-controlled co-metabolism decomposition of difficultly degraded phenol pollutants

Examples

Experimental program
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Effect test

Embodiment 1

[0021] In this embodiment, intermittent co-substrate regulation and co-metabolism decomposes refractory phenolic pollutants is achieved in this way:

[0022] 1. Adopt 250ml serum bottle to carry out experiment, the bacterial concentration of inoculation anaerobic sludge is 5g / L, the concentration of pentachlorophenol (PCP) is 9mg / L, and the total volume of water sample in the bottle is 180ml.

[0023] 2. Divide the experiments into two groups, group A and group B, each group has two parallel samples, and all serum bottles are cultured in a constant temperature shaker. The shaking speed was 120r / min, the culture temperature was 35°C, the pH was about 7.0, and the nitrogen and phosphorus concentrations in all water samples were 50mg / L and 10mg / L, respectively. The water is controlled to contain no dissolved oxygen by blowing off nitrogen, and it is in an anaerobic state.

[0024] 3. Group A experiments used pentachlorophenol alone as the substrate, group B experiments used sodi...

Embodiment 2

[0031] In this embodiment, intermittent co-substrate regulation and co-metabolism decomposes refractory phenolic pollutants is achieved in this way:

[0032] 1. A 250ml serum bottle was used for the experiment. The bacterial concentration inoculated with the aerobic sludge was 0.5g / L, the o-cresol (o-MP) concentration was 30mg / L, and the total volume of the water sample in the bottle was 180ml.

[0033] 2. Divide the experiments into two groups, group A and group B, each group has two parallel samples, and all serum bottles are cultured in a constant temperature shaker. The shaking speed was 120r / min, the culture temperature was 20°C, the pH was about 7.0, and the nitrogen and phosphorus concentrations in all water samples were 50mg / L and 10mg / L, respectively. The dissolved oxygen concentration is 0.5-5mg / L.

[0034] 3. The group A experiment used o-cresol alone as the substrate, and the group B experiment used glucose and o-cresol as the substrate, and the initial dosage con...

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Abstract

The invention discloses a method for intermittent co-substrate-controlled co-metabolism decomposition of difficultly degraded phenol pollutants. The method is characterized in that co-substrate solutions are intermittently added into water containing phenol pollutants in a concentration decreasing way, wherein a weight ratio of co-substrates in the first added co-substrate solution to the phenol pollutants in the water containing the phenol pollutants is 10-500: 1, a time pace of the co-substrate solution filling comprises 1-7 days, a weight ratio of the second added co-substrate solution to the first added co-substrate solution is 1 / 10-9 / 10, a weight ratio of the third added co-substrate solution to the second added co-substrate solution is 1 / 10-9 / 10 and the rest can be done in the same manner until the concentration of the phenol pollutants in the water is reduced in an allowable scope. The method has the advantages of flexible operation, wide application range, low operation cost, good treatment effects and large application prospect.

Description

1. Technical field [0001] The invention relates to a biodegradation method for refractory phenolic pollutants in water bodies, in particular to a method for intermittent co-substrate regulation and co-metabolism to decompose refractory phenolic pollutants, belonging to the field of water environment pollution control. 2. Background technology [0002] Refractory phenolic pollutants have the characteristics of strong toxicity and environmental persistence, and are the key pollutants controlled by environmental protection departments at home and abroad. If people drink surface water containing phenolic pollutants for a long time, it will cause dizziness, anemia and various nervous system diseases. When the content of phenolic pollutants in water is greater than 10mg / L, various metabolic activities of microorganisms will be significantly inhibited. It is of great significance to carry out the research on the treatment of refractory phenolic pollutants to protect the environmen...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F101/34
Inventor 王伟张静潘尚磊王顺沈俊徐瑞捷李德来陈益徐玮键许福文
Owner HEFEI UNIV OF TECH
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