Application of thymopentin in preparation of medicine for treating mastitis

A technology for dairy cow mastitis and recessive mastitis, which is applied in the direction of drug combination and diseases, and can solve problems such as no thymopentin, drug resistance of cow mastitis bacteria, and exacerbation of the disease

Active Publication Date: 2015-11-25
ZHEJIANG HUAERCHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of antibiotics mainly has the following disadvantages: First, long-term use of antibiotics will produce drug resistance to cow mastitis bacteria, causing the generation of superbugs
The second is frequent use of antibiotics, once the drug is stopped, the condition will aggravate
Third, long-term use of antibiotics will affect the milk quality of milk produced by cows. Milk contains a large amount of antibiotics, which endangers human food safety.
But there is no relevant report on the application of thymopentin in the preparation of mastitis, especially cow mastitis medicine

Method used

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  • Application of thymopentin in preparation of medicine for treating mastitis
  • Application of thymopentin in preparation of medicine for treating mastitis
  • Application of thymopentin in preparation of medicine for treating mastitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Rapid diagnosis of hidden mastitis in dairy cows (HMT): Discard the first three handfuls of milk before field sampling. Squeeze the milk from the 4 udder areas of the examined cow into the 4 chambers of the diagnostic tray, each chamber is about 2ml, and add 2ml of HMT diagnostic reagent to each chamber. Shake the diagnostic plate to mix the milk and reagents thoroughly and judge the result. The judgment criteria are shown in Table 1.

[0021] Table 1 Judgment criteria for recessive mastitis in dairy cows

[0022]

[0023] Somatic cell counting method: (1) Calibrate the milk somatic cell counter (FossomaticTM Minor, Denmark) with the standard product provided by Foss; (2) Heat the test milk sample (about 50ml) in a water bath to about 40°C and mix well Milk, tested with a somatic cell counter.

[0024] Case selection: Jinjiang dairy farm in the suburbs of Hangzhou, 120 black and white cows, with an average age of 5.5 years. Use Hangzhou Mastitis Detection Reagent ...

Embodiment 2

[0035] Method for rapidly diagnosing dairy cow latent mastitis (HMT): same as Example 1.

[0036] Somatic cell counting method: with embodiment 1.

[0037] Bacteriological inspection: According to the NMC (National Mastitis Council) recommended procedures for milk bacteriological inspection. The specific operation steps are as follows: (1) First, about 10 μL of milk samples were picked and inoculated on sheep blood plate medium, and incubated at a constant temperature of 37°C for 24-48 hours. According to the morphological characteristics of the colonies, pick suspicious colony smears, Gram staining, and microscopic examination; (2) According to the bacterial growth rate, colony morphology, and staining characteristics, they can be preliminarily divided into three categories, namely, Gram-positive cocci, Gram-positive cocci, and Gram-positive cocci. Positive bacilli, Gram-negative bacilli; (3) Gram-positive cocci were divided into staphylococci and streptococci by catalase te...

Embodiment 3

[0064] Case selection of clinical mastitis: Jinjiang dairy farm in the suburbs of Hangzhou, 120 black and white cows, with an average age of 5.5 years. The dairy cows with red, swollen udders and low milk production were screened out visually, and the milk samples from the milk area of ​​each cow were tested with Hangzhou Mastitis Testing Reagent (HMT). There are 15 dairy cows and 15 milking areas in the gray-white milking area.

[0065] Thymopentin for injection: Thymopentin provided by Zhejiang Huaercheng Biopharmaceutical Co., Ltd., each containing 2 mg thymopentin, batch number 20110401.

[0066] Grouping and treatment: 15 test cows were randomly divided into 3 groups: middle and high doses of test drug group, and blank control group, with 5 cases in each group. The three groups of patients in the test group were respectively injected with thymopentin 10 mg (medium dose group) and 20 mg (high dose group) into the supramammary lymph nodes on one side of the affected breast...

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Abstract

The invention relates to the field of medicine, and especially relates to an application of thymopentin in preparing medicine used for treating mastitis. As a result of subclinical mastitis efficacy test, infection mammary area numbers of thymopentin treatment groups, especially a high dose (4mg) group, have a reduction tendency after treatment. As a result of clinical mastitis efficacy test, through injection of thymopentin (TP-5) in breast lymph node for continuously 3 days, milk SCC of two TP-5 dose groups (10mg and 20mg) both have reduction tendencies, and cow breast swellings are substantially ameliorated in appearance. Therefore, medicines prepared with high-dose thymopentin (higher than 10mg) have significant effects against cow clinical mastitis. Therefore, the medicines prepared by using thymopentin have significant effects against subclinical mastitis and / or clinical mastitis.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the application of thymopentin in the preparation of medicines for treating mastitis. Background technique [0002] Mastitis is mainly an inflammation caused by bacterial infection of the breast tissue. The nipple duct is a main way for bacteria to invade the mammary gland and cause infection. In a healthy state, the epithelial cells of the nipple duct secrete keratin and other substances with antibacterial effects, which are the first line of defense for bacteria to invade the mammary gland. When the teat duct is damaged due to milking and other reasons, the function of epithelial cells to secrete antibacterial substances is destroyed, and bacteria can easily enter the mammary gland. When bacteria break through the physical barrier of the nipple and invade the mammary gland, the second line of defense encountered is the humoral and cellular defense factors in the mammary gland. Humora...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/08A61P15/14
Inventor 周正兵王祖兵贾代宏曾祖丽
Owner ZHEJIANG HUAERCHENG PHARMA
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