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Preparation process of chicken Eimeria tenella yolk antibody targeted sustained-release preparation

A kind of Emy ear ball and antibody targeting technology, which is applied in the direction of antibodies, anti-infective drugs, and medical preparations with non-active ingredients, etc., to avoid the use of weak specificity, improve pharmacokinetic characteristics, and reduce direct economic losses Effect

Active Publication Date: 2016-05-04
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem of chicken coccidiosis prevention and treatment, the present invention provides a preparation process of Eimeria tenella yolk antibody targeted slow-release preparation

Method used

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  • Preparation process of chicken Eimeria tenella yolk antibody targeted sustained-release preparation
  • Preparation process of chicken Eimeria tenella yolk antibody targeted sustained-release preparation
  • Preparation process of chicken Eimeria tenella yolk antibody targeted sustained-release preparation

Examples

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Embodiment 1

[0038] Example 1. A preparation process for a targeted slow-release preparation of Eimeria tenella yolk antibody. The steps are: (1) Preparation, separation and purification of highly immune yolk antibody (IgY). The specific steps are: by giving Healthy Chicks Against Viruses E. tenella , use the chickens to subculture and amplify to obtain the antigen; then give the antigen orally to the healthy laying hens, immunize once every 8 days, and immunize three times in total, and collect the high-immunity eggs for the last immunization; separate the yolk from the high-immunity eggs to obtain the yolk stock solution; the yolk stock solution is used for pre-treatment Dilute with cold sterilized distilled water at a volume ratio of 1:6, adjust the pH to 4.8 with 0.1mol / L HCL, stir well, let stand at 4°C for 6h, centrifuge at 10,000r / min for 15min, and collect the supernatant as egg yolk water-soluble fraction (WFS ); using caprylic acid-cold ethanol extraction method or ammonium sulfa...

Embodiment 2

[0041] A preparation process for chicken Eimeria tenella yolk antibody targeted sustained-release preparation, the steps are: (1) preparation, separation and purification of highly immune yolk antibody (IgY), the specific steps are: challenge healthy chicks with the virus E. tenella , use the chickens to subculture and amplify to obtain the antigen; then give the antigen orally to the healthy laying hens, immunize once every 10 days, and immunize three times in total, and collect the highly immune eggs for the last immunization; separate the yolk from the highly immune eggs to obtain the yolk liquid; Dilute with cold sterilized distilled water at a volume ratio of 1:9, adjust the pH to 5.1 with 0.1mol / L HCL, stir well, let stand at 4°C for 8h, centrifuge at 10,000r / min for 20min, and collect the supernatant as egg yolk water-soluble fraction (WFS ); using caprylic acid-cold ethanol extraction method or ammonium sulfate secondary precipitation method to separate and extract IgY ...

Embodiment 3

[0044] A preparation process for chicken Eimeria tenella yolk antibody targeted sustained-release preparation, the steps are: (1) preparation, separation and purification of highly immune yolk antibody (IgY), the specific steps are: challenge healthy chicks with the virus E. tenella , use the chickens to subculture and amplify to obtain the antigen; then give the antigen orally to the healthy laying hens, and immunize once every 12 days, a total of 3 times of immunization, and collect the high-immune eggs for the last immunization; separate the yolk from the high-immune eggs to obtain the yolk stock solution; Dilute with cold sterilized distilled water at a volume ratio of 1:10, adjust the pH to 5.5 with 0.1mol / L HCL, stir well, let stand at 4°C for 10 hours, centrifuge at 10,000r / min for 25 minutes, and collect the supernatant as egg yolk water-soluble fraction (WFS ); using octanoic acid-cold ethanol extraction method or ammonium sulfate secondary precipitation method to sepa...

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Abstract

The invention relates to a chicken coccidiosis prevention and treatment preparation, in particular to a preparation technology of a chicken Eimeria tenella egg yolk antibody targeted sustained release preparation so as to solve the prevention and treatment problems of chicken coccidiosis. The preparation technology of the chicken Eimeria tenella egg yolk antibody targeted sustained release preparation includes the steps of: preparation, separation and purification of a highly immunized egg yolk antibody (IgY), and preparation of a microcapsule. Preparation of the microcapsule has the reaction conditions that: the chitosan concentration is 0.05-0.8% (w / v), the concentration of CaCl2 is 0.5-1.5% (w / v), the concentration of sodium alginate is 1.0-3.0% (w / v), and the IgY and the sodium alginate are in a mass ratio of 1-4:8. The IgY microcapsule prepared by the technical scheme involved in the invention is a preventive medicine, and is also a therapeutic drug. The microcapsule prepared by the invention provides a very good example for antibiotic substitution therapy. And the determined technology can also be used to prepare IgY or IgG microcapsules of other pathogenic microorganisms, thus having promotional value, and being beneficial to sound development of the green and organic chicken raising industry.

Description

technical field [0001] The invention relates to a preparation for preventing and treating coccidiosis in chickens, in particular to a preparation process for a targeted slow-release preparation of Eimeria tenella yolk antibody. Background technique [0002] Chicken coccidiosis (coccidiosis) is a parasitic protozoan disease caused by one or several species of Eimeria coccidiosa of the genus Eimeria in the genus Eimeria in the chicken intestine. Coccidiosis is a common and serious disease. According to reports, there are 9 species of Eimeria parasitized in chickens, among which Eimeria tenella ( E. tenella ), Eimeria heap type ( E. acervulina ), Eimeria giga ( E. maxima ), poisonous Eimeria ( E. necatrix ), Eimeria brucei ( E. brunetti ) five kinds. Eimeria tenella is the most common and most pathogenic type of coccidia in chickens, and it mainly infects chicken cecum and its surrounding areas. The incidence rate of 15-30-day-old chicks is the highest, up to 50%-70%...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/395A61K9/50A61K47/36A61P33/02
Inventor 霍乃蕊张新荣巩红霞李宝霞李芳王珺任志远韩克光郑明学古少鹏
Owner SHANXI AGRI UNIV
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