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Rapid propagation method for bletilla striata

A bletilla striata, rapid technology, applied in the planting field of plant diseases, can solve problems such as the decline of wild resources and the increase in demand, and achieve the effects of simple treatment, enhanced survival rate, and high survival rate

Active Publication Date: 2013-10-16
JIANGSU MAODISHANDAO CHINESE MEDICINAL HERBAL PLANTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that in recent years, affected by the strong growth of medicinal demand and horticultural demand, the market demand for Bletilla striata in my country has also increased significantly; over-harvesting and habitat destruction have led to a sharp decline in the amount of wild resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A rapid propagation method of Bletilla striata includes the following steps:

[0025] 1) Selection of planting land: choose shady sloping wasteland, plow the ground with a depth of 30cm, raking and leveling, and build a group of 130cm wide and 20cm high borders, superphosphate and decomposed farm manure as base fertilizer;

[0026] 2) Selection and disinfection of explants: During the harvest season of Bletilla striata, the false bulbs with tender buds and old bulbs were cut off adjacent to the old bulbs, disinfected with 0.5% potassium permanganate, 15 minutes later in 75% alcohol Soak for 10 minutes, soak with 1% sodium hypochlorite for 5 minutes, then rinse with sterile water, cut off the discolored part and set aside;

[0027] 3) Sprout germination: inoculate the explants sterilized in step 2) into MS+0.5 mg / L BA+0.01 mg / L NAA medium, pH 5.8, culture temperature 25°C, and 8 light per day Hours, the light intensity is 900LX, and they will grow into seedlings 10-15 days aft...

Embodiment 2

[0037] A rapid propagation method of Bletilla striata includes the following steps:

[0038] 1) Selection of planting land: choose the uncultivated land on the shady slope, the depth of plowing is 40cm, the rake is fine and leveled, and a set of 140cm wide and 30cm high borders are built, superphosphate and decomposed farm manure are used as base fertilizer;

[0039] 2) Selection and disinfection of explants: During the harvest season of Bletilla striata, the pseudobulbs with tender buds and old bulbs were cut off adjacent to the old bulbs, disinfected with 3% potassium permanganate, 15 minutes later in 75% alcohol Soak for 10 minutes, soak with 1% sodium hypochlorite for 5 minutes, then rinse with sterile water, cut off the discolored part and set aside;

[0040] 3) Sprout germination: inoculate the explants sterilized in step 2) into MS+0.5 mg / L BA+0.01 mg / L NAA medium, pH 5.8, culture temperature 25°C, and 8 light per day Hours, the light intensity is 900LX, and they will grow in...

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Abstract

The invention discloses a rapid propagation method for bletilla striata. The method comprises the following steps of: selecting cultivated fields; selecting and disinfecting explants; sprouting tender shoots; proliferating multiple shoots; carrying out rooting culturing; transplanting plant seedlings, transplanting hardened seedlings; and carrying out field management. According to the rapid propagation method for the bletilla striata, the explants are selected to be proliferated, the processing manner is simple and has an environment-friendly effect, poisonous agents are not utilized for carrying out disinfection treatment, sprouts of the bletilla striata are not injured, and the surviving rate of the sprouts of the bletilla striata is improved by utilizing the processing method; different culture media are utilized in different stages, and the surviving rate is relatively high; conditions of each stage are groped by planting, and an excellent bletilla striata variety is finally obtained.

Description

Technical field [0001] The invention belongs to the field of plant disease planting, and specifically relates to a method for rapid propagation of Bletilla striata. Background technique [0002] Bletilla striata is widely distributed in various provinces of my country's Yangtze River Basin, mainly produced in Guizhou, Sichuan, Hunan, Hubei, Anhui, Henan, Zhejiang, Shaanxi and other places. Most of them are wild, but a small amount of cultivation is also available, and harvested in 3 to 4 years. [0003] Baiji prefers a cool, humid and warm environment. The annual average temperature in Sichuan is around 17°C, the precipitation is 1,100-1,200 mm, the absolute maximum temperature is 38°C, and the absolute minimum temperature is -6°C. Can grow normally. Asexual reproduction can also be used for sexual reproduction. Because the seeds are small and it is difficult to germinate, seed reproduction is generally not used. [0004] Bletilla striata (Thunb.ex A. Murray) Reichb.f.] belongs to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 经守萄
Owner JIANGSU MAODISHANDAO CHINESE MEDICINAL HERBAL PLANTING
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