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Method for preparing magnetized and hydroformyled sheep red blood cell

A technology of aldehydizing sheep red blood cells and glutaraldehyde, which is applied in the biological field, can solve the problems of harsh experimental conditions, low protein labeling efficiency, and uneven particle size, and achieve low cost, rapid aggregation and redispersion, and uniform size Effect

Active Publication Date: 2015-06-24
SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, some microspheres are made of synthetic or natural polymer materials for the detection of soluble antigens and antibodies, but the synthesis process of this type of microspheres is cumbersome, the experimental conditions are harsh, the price of purchased finished products is high, and there are particles of different sizes. Uniformity, and low efficiency of protein labeling, so it has not been widely used clinically

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  • Method for preparing magnetized and hydroformyled sheep red blood cell

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Embodiment 1

[0031] Provides an agglutination test model using aldehyde-magnetized sheep erythrocytes to detect soluble antibodies, detects other specific antibodies, and coats the corresponding specific antigens on aldehyde-magnetized sheep erythrocytes, which can be applied to antibody detection. Including the following steps:

[0032] 1. Preparation of nanomagnetic beads

[0033] 0.85g FeCl 3 ·6H 2 O and 0.30 g FeCl 2 4H 2 O was dissolved in 200 mL of distilled water under nitrogen protection. Add an appropriate amount of surfactant, and slowly add 1.5 mol / L ammonia solution to the above solution under strong stirring. When the pH of the solution rises to 6~7, a large amount of black Fe will be produced in the solution. 3 o 4 Particles; continue to add ammonia water to pH = 8, so that the hydrolysis is complete. Aged at 80°C for 0.5 h. The resulting solid was separated, washed 3 times with distilled water, and then dispersed in 100 mL of distilled water under the action of ultra...

Embodiment 2

[0057] The invention provides a test model of using aldehyde-magnetized sheep erythrocytes for antibody fluorescence detection, in which specific antigens are coated on aldehyde-magnetized sheep erythrocytes for antibody detection. Including the following steps:

[0058] 1. Preparation of nano magnetic beads

[0059] 0.85g FeCl 3 ·6H 2 O and 0.30 g FeCl 2 4H 2 O was dissolved in 200 mL of distilled water under nitrogen protection. Add an appropriate amount of surfactant, and slowly add 1.5 mol / L ammonia solution to the above solution under strong stirring. When the pH of the solution rises to 6~7, a large amount of black Fe will be produced in the solution. 3 o 4 Particles; continue to add ammonia water to pH = 8, so that the hydrolysis is complete. Aged at 80°C for 0.5 h. The resulting solid was separated, washed 3 times with distilled water, and then dispersed in 100 mL of distilled water under the action of ultrasound to obtain Fe 3 o 4 The colloidal solution was ...

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Abstract

The invention discloses a method for preparing a magnetized and hydroformyled sheep red blood cell. The method comprises the following steps of: preparing nano magnetic beads; preparing a hydroformyled sheep red blood cell; preparing a magnetized and hydroformyled sheep red blood cell; coating protein on the magnetized and hydroformyled sheep red blood cell; detecting a sample; and observing the result. The prepared magnetized and hydroformyled sheep red blood cell can be used as an indicator cell of an agglutination test, a carrier, enzyme and a chemiluminescence matter of an antigen antibody agglutination test, or a carrier detected in a fluorescent material marking test. The magnetized and hydroformyled sheep red blood cell prepared by the method is long in retention period, simultaneously has paramagnetism, can achieve rapid aggregation and redispersion, avoids complicated centrifuge processes in the traditional agglutination test, and can be used for preparing artificial granular antigen antibodies with uniform sizes; coating and detection reaction are carried out in a uniform phase after a solid-phase carrier is replaced; and the method is full in action, high in sensitivity, low in cost than an elisa plate, and is mainly applied to a screening or quantitative detection test of a special red blood cell antibody and other soluble antigen antibodies.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing magnetically-formylated sheep erythrocytes. Background technique [0002] Agglutination reaction is a serological reaction, the process is the combination of particulate antigens (intact pathogenic microorganisms or red blood cells, etc.) It can be divided into direct agglutination reaction and indirect agglutination reaction. [0003] Direct agglutination is the agglutination phenomenon that occurs when granular antigens (such as bacteria, red blood cells, etc.) are directly combined with corresponding antibodies, and it is mainly divided into slide method and test tube method. The slide method is a qualitative test method in which known antibodies can be used to detect unknown antigens. The test tube method is a classic method of quantitative testing. Known antigens can be used to detect the presence or absence of a certain antibody and the content of the a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00
Inventor 王红梅李勇田晶晶丁少华段生宝陈烨洲史素霞李冬
Owner SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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