Cultivation method of novel dammarane-type ginsenoside-containing paddy rice variety

Abstract

The invention discloses a cultivation method of a novel dammarane-type ginsenoside-containing paddy rice variety and belongs to the field of genetic engineering. The breeding method is characterized in that a full-length cDNA of a gene of a dammarenediol synthetase as a key enzyme of dammarane-type ginsenoside biosynthesis is constructed to double-T-DNA plant expression vectors; the constructed product is subjected to genetic transformation in paddy rice; and through screening cultivation, the novel dammarane-type ginsenoside-containing paddy rice variety is obtained. A test proves that the novel dammarane-type ginsenoside-containing paddy rice variety comprises 0.020% of 20(S)-protopanoxadiol (PPD) and 0.173% of 20(S)-protopanaxatriol (PPT). The cultivation method can be used for cultivation of high-quality ginseng rice varieties containing dammarane-type ginsenoside, can improve a health care value of rice as a main food of a human body, can widen a ginsenoside source, can alleviate the scarcity of a ginsenoside drug source, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of crop genetic engineering, and in particular relates to a method for producing rice containing dammarane-type ginsenosides. Background technique [0002] Rice is one of the main food crops in the world, and nearly half of the world's population uses rice as a staple food. Traditional breeding technology has made a great contribution to improving rice quality, but because it is difficult to overcome the barriers of reproductive isolation between species, it is difficult to significantly improve rice quality, especially the health quality of rice. The rapid development of modern genetic engineering technology provides an effective means for the genetic improvement of rice quality, that is, through genetic engineering, new metabolite synthesis branches can be introduced into rice, so that rice can synthesize new functional components, thereby improving rice quality. nutritional health value. [0003] Ginseng ( Panax...

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Problems solved by technology

But so far there is no literature report on the transformation of Panax dammarenediol synthase gene into rice and the creation of new rice germplasm containing dammarane-type ginsenosides

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