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A kind of ultra-low temperature freezing process of probiotics and its application in probiotic preparations

A technology of ultra-low temperature freezing and probiotic preparations, which is applied in the fields of bacteria and food science, and can solve the problems of reducing the number of viable probiotic bacteria and damage to the activity of probiotic strains, and achieves fast freezing speed, easy preservation, and high rate of viable bacteria. Effect

Active Publication Date: 2017-03-15
THANKCOME BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing probiotic strains generally exist in the form of freeze-dried powder. When reprocessing the freeze-dried powder of probiotics, it inevitably involves rehydration, wetting, stirring and mixing with other materials. When these operations are performed, the activity of probiotic strains will be damaged, and the number of viable bacteria of probiotics will be reduced to varying degrees.

Method used

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  • A kind of ultra-low temperature freezing process of probiotics and its application in probiotic preparations
  • A kind of ultra-low temperature freezing process of probiotics and its application in probiotic preparations

Examples

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Embodiment 1

[0057] Example 1 Preparation of Probiotic Ultra-low Temperature Frozen Granules

[0058] The preparation method of probiotic ultra-low temperature frozen granules described in this embodiment is as follows:

[0059] (1) Preparation of fermentation medium: configure 5L of medium, by weight percentage, the proportion of each component in the medium is lactose 1%, glucose 2%, peptone 2%, yeast extract 1%, diammonium hydrogen citrate 0.5% 、K 2 HPO 4 0.1%, 0.5% anhydrous sodium acetate, 0.02% magnesium sulfate, the rest is distilled water, adjust the pH to 6.5, after preparation, sterilize at 121°C for 20 minutes, and set aside;

[0060] (2) Cultivate probiotics: Inoculate Lactobacillus bulgaricus, Streptococcus thermophilus, and Bifidobacterium lactis into the above-mentioned culture medium according to the inoculum amount of 5%, and cultivate them at a temperature of 37°C and a rotation speed of 50rpm for 16 hours, and then in Place at 15°C for 3 hours to obtain a probiotic fe...

Embodiment 2

[0067] Embodiment 2 A kind of yogurt starter granules that can be directly taken orally

[0068] A kind of preparation method of the directly oral yoghurt starter granule of the present embodiment comprises the following steps:

[0069] (1) Prepare 10 kg of compound raw materials, including dry materials and two kinds of probiotic ultra-low temperature frozen granules prepared in Example 1. The composition of dry materials is 2 kg of starch, 1 kg of sweet orange powder, 3 kg of erythritol, 2 kg of lactose, β -Polydextrose 1kg, all raw materials are passed through 80 mesh sieves to form fine powder for granulation;

[0070] (2) Mix the above dry materials evenly in the mixer;

[0071] (3) Under the storage condition of -20°C, take out 0.4kg of the ultra-low temperature frozen starter granules prepared in Example 1 and 0.6kg of the bifidobacterium granules prepared in Example 1, and melt at room temperature to re-form the bacterial suspension to obtain 0.4kg Bacterial suspensi...

Embodiment 3

[0075] Example 3 Preparation of Probiotic Ultra-low Temperature Frozen Granules

[0076] The preparation method of probiotic ultra-low temperature frozen granules described in this embodiment is as follows:

[0077] (1) Preparation of fermentation medium: 5L of medium is prepared, and the distribution ratio of each component in the medium is 2% lactose, 1% glucose, 1% peptone, 2.5% yeast extract, and 1% diammonium hydrogen citrate 、K 2 HPO 4 0.5%, 0.1% anhydrous sodium acetate, 0.05% magnesium sulfate, the rest is distilled water, adjust the pH to 6.5 with NaOH solution, sterilize at 121°C for 20 minutes after preparation, and set aside;

[0078] (2) Cultivate probiotics: Inoculate Lactobacillus acidophilus, Streptococcus thermophilus, and Lactobacillus rhamnosus into the above-mentioned culture medium according to the inoculation amount of 5%, and cultivate them at a temperature of 37°C for 16 hours, with a stirring speed of 50rpm , and then placed at 15°C for 5 hours to o...

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Abstract

The invention discloses a probiotic ultralow temperature refrigeration technology, a formula of a total-salt cryoprotectant which is suitable for the probiotic ultralow temperature refrigeration technology, and probiotic ultralow temperature refrigeration particles prepared by using the probiotic ultralow temperature refrigeration technology. The probiotic ultralow temperature refrigeration technology comprises following steps: (1) preparing a fermentation medium; (2) culturing probiotics; (3) centrifuging; (4) preparing the cryoprotectant; (5) adding the cryoprotectant; and (6) quick-freezing and moulding. The invention also provides a probiotic preparation prepared by using the probiotic ultralow temperature refrigeration particles, and a preparation method of the probiotic preparation. The probiotic ultralow temperature refrigeration particles are probiotic refrigerated bacterial strains which do not need to be dried and possesses advantages of fast refrigeration speed, high living bacterial rate and excellent rehydration performance. The probiotic ultralow temperature refrigeration particles contain water, can be dissolved in solutions more quickly, and are capable of preventing probiotic cells from damage caused by rehydration of normal low temperature refrigeration dried probiotic powder, and increasing the living bacterial rate of the probiotic preparation greatly.

Description

technical field [0001] The invention belongs to the field of production technology of probiotic preparations, and relates to a frozen storage technology for secondary processing of probiotics and its specific application in the production process of probiotic preparations. Background technique [0002] During the preparation of probiotic preparations, especially when the product appears in the form of granules or tablets, reprocessing of probiotic strains is often involved. Existing probiotic strains generally exist in the form of freeze-dried powder. When the probiotic freeze-dried powder is reprocessed, it inevitably involves rehydration, wetting, and stirring and mixing with other materials. When these operations are carried out, the activity of the probiotic strains will be damaged, and the number of viable bacteria of the probiotics will be reduced to varying degrees. [0003] Therefore, developing a probiotic strain with a high rate of viable bacteria and good rehydra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23L33/00
Inventor 喻扬
Owner THANKCOME BIOLOGICAL SCI & TECH CO LTD
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