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Method for establishing fish inflammatory bowel disease model and established model thereof

A technology for inflammatory bowel disease and model building, which is applied in the field of fish inflammatory bowel disease model building methods and model building, can solve the problems of mechanical damage, adverse effects, and disease of fish bodies, and achieve repeatability and accuracy. Good stability, avoid mechanical damage, wide infection spectrum

Active Publication Date: 2013-09-18
苏州培恩特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aeromonas hydrophila cannot infect healthy fish by oral or immersion, but intraperitoneal or intramuscular injection must be used. However, intraperitoneal or intramuscular injection will cause mechanical damage to the fish body, and intraperitoneal or intramuscular injection will cause The pathogenic bacteria may enter the blood and lead to systemic disease in the fish along with the blood circulation. The inflammatory bowel disease model constructed is not targeted and has a negative impact on subsequent research.
At present, there is no method that can cause inflammation through non-mechanical injury such as oral administration, soaking or anal perfusion

Method used

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  • Method for establishing fish inflammatory bowel disease model and established model thereof
  • Method for establishing fish inflammatory bowel disease model and established model thereof
  • Method for establishing fish inflammatory bowel disease model and established model thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Grass carp inflammatory bowel disease model establishment

[0060] 240 grass carp with a size of 20g to 50g were taken and raised in the laboratory for 20 days to establish an inflammatory bowel disease model. The experimental fish were divided into 4 groups, namely the experimental group infused with Aeromonas hydrophila, the negative control group infused with 0.7% normal saline, the positive control group infused with TNBS, and the blank control group, with 60 fish in each group.

[0061] The preparation method of the TNBS solution is as follows: the volume fraction of 5% TNBS is mixed with absolute ethanol in equal volumes to obtain the product.

[0062] Prepare a MS-222 solution with a mass-volume concentration of 50 mg / L, soak and anesthetize the experimental fish.

[0063] The fish in the experimental group were perfused with the Aeromonas hydrophila solution with the preservation number CCTCC NO.M2013089 through the anus, and the concentration was 10 ...

Embodiment 2

[0066] Example 2 Establishment of crucian carp inflammatory bowel disease model

[0067] 240 crucian carp with a size of 20g-50g were taken and bred in the laboratory for 20 days to establish an inflammatory bowel disease model. The experimental fish were divided into 4 groups, namely the experimental group infused with Aeromonas hydrophila, the negative control group infused with 0.7% normal saline, the positive control group infused with TNBS, and the blank control group, with 60 fish in each group.

[0068] The preparation method of the TNBS solution is as follows: the volume fraction of 5% TNBS is mixed with absolute ethanol in equal volumes to obtain the product.

[0069] Prepare a MS-222 solution with a mass-volume concentration of 70 mg / L, soak and anesthetize the experimental fish.

[0070] The fish in the experimental group were perfused with the Aeromonas hydrophila solution with the preservation number CCTCC NO.M2013089 through the anus, and the concentration was 1...

Embodiment 3

[0073] Example 3 Grass carp inflammatory bowel disease model establishment

[0074] The inflammatory bowel disease model was established after 300 grass carp with a size of 50g-100g were raised in the laboratory for 20 days. The experimental fish were divided into 4 groups, namely the experimental group infused with Aeromonas hydrophila, the negative control group infused with 0.7% normal saline, the positive control group infused with TNBS, and the blank control group, with 100 fish in each group.

[0075] The preparation method of the TNBS solution is as follows: the volume fraction of 5% TNBS is mixed with absolute ethanol in equal volumes to obtain the product.

[0076] Prepare a MS-222 solution with a mass-volume concentration of 70 mg / L, soak and anesthetize the experimental fish.

[0077] The fish in the experimental group were perfused with the Aeromonas hydrophila solution with the preservation number CCTCC NO.M2013089 through the anus, and the concentration was 10 ...

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Abstract

The invention relates to the technical field of establishment of animal experiment models and in particular relates to a method for establishing a fish inflammatory bowel disease model and the established model thereof. The method comprises the following steps: injecting aeromonas hydrophila with the collection number of CCTCC NO.M2013089 into a soaked anaesthetic healthy fish through anus perfusion, breeding and selecting fish with the symptom of the inflammatory bowel disease as the fish inflammatory bowel disease model. According to the method, the aeromonas hydrophila is directly filled in the intestinal tract of the fish, and the process that the pathogenic bacteria enter blood through intramuscular injection or intraperitoneal injection and act on the intestinal tract through blood circulation is avoided, so that the pathogenic bacteria directly act on the intestinal tract, and the pertinence is improved; furthermore, the established model has high stability and repeatability, and because the aeromonas hydrophila is wide in infection spectrum, the established method can be widely applied to multiple fishes.

Description

technical field [0001] The invention relates to the technical field of animal experiment model building, in particular to a method for building a fish inflammatory bowel disease model and the model built therefor. Background technique [0002] Fish deliver a large amount of animal protein nutrition to Chinese consumers, but the occurrence frequency of inflammatory bowel disease in the breeding process is extremely high, usually fish bacterial enteritis is associated with red skin disease, gill rot disease, and intestinal hexapiasis , intestinal bagworm, viral hemorrhagic disease, hepatobiliary syndrome and other diseases, seriously affect its survival rate and aquaculture production performance, and hinder the sustainable development of fish aquaculture industry. The mechanism of fish enteritis is still unclear. Therefore, it is of great theoretical and practical significance to study the main cause, symptoms and pathological mechanism of fish inflammatory bowel disease (IBD...

Claims

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Application Information

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IPC IPC(8): A61K35/74
Inventor 宋学宏赵杰孙丙耀
Owner 苏州培恩特生物科技有限公司
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