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Artificial schisandra chinensis seed preparation and seedling culture method

A technology of artificial seeds and Schisandra chinensis, applied in the field of medicine, can solve the problems of low reproduction coefficient, long aseptic operation process, labor and material resources consumption, etc.

Inactive Publication Date: 2013-09-18
LIAONING WANMU SCHISANDRA TECH IND PARK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the advantages of low technical difficulty and high success rate, but the reproduction coefficient is low, the reproduction speed is slow, the aseptic operation process is long, the labor intensity is high, and it also requires a lot of manpower and material resources.

Method used

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  • Artificial schisandra chinensis seed preparation and seedling culture method

Examples

Experimental program
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Effect test

example 1

[0021] 1-4 steps of operation refer to the content of the invention part (1) ~ (4), that is, the explant of the fine variety Schisandra chinensis is used as the starting material, after the surface is sterilized successively by 75% alcohol and saturated sodium hypochlorite solution, it is cut into small pieces and placed in the ultra-clean Inoculate the workbench containing 0.5-2 mg / L of 2,4-dichlorophenoxyacetic acid, 1.0-3.0 mg / L of 6-benzylaminopurine, 15-30 g / L of sucrose, and 0.5-1% of agar, and adjust the final pH to Callus induction was carried out in sterile MS solid medium (callus induction medium) between 5.5 and 6.5 at 25±1° C. under dark culture conditions.

[0022] Select light yellow-green, loose texture, and vigorously growing callus, inoculate it into the callus induction medium without agar, establish its suspension culture condition, and obtain vigorous growth and uniform cell suspension.

[0023] After obtaining a sufficient amount of cell suspension, filter...

example 2～5

[0027] With reference to Example 1 step, change the concentration / ratio of each variable and carry out the preparation and seedling cultivation experiment of artificial seeds, the results are as follows:

[0028]

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Abstract

The invention belongs to the technical field of pharmaceuticals, and relates to an artificial schisandra chinensis seed preparation and seedling culture method. The method can be used for normally germinating artificial schisandra chinensis seeds in a bacterial culture medium. The artificial schisandra chinensis seeds can be prepared according to the following process: inoculating excellent schisandra chinensis explants into a callus induction medium so as to obtain callus, carrying out embryonic type callus induction on the callus, carrying out somatic embryo induction on the embryonic type callus, synchronously culturing and subculturing somatic embryos obtained through induction, carrying out ion exchanging on cotyledon phase somatic embryos so as to form sodium alginate-CaCl2 hydrogel spheres, and wrapping the obtained hydrogel spheres by utilizing deacetylation chitosan and carbendazol micro powder so as to obtain the artificial seeds wrapped by artificial seed coats; and germinating the artificial seeds so as to obtain the schisandra chinensis. The method can be used for greatly culturing seedlings in a short time and is labor-saving and material-saving.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for preparing artificial seeds of Schisandra chinensis and raising seedlings. Background technique [0002] Schisandra chinensis is an authentic medicinal material in Northeast China. Among them, Liaoning Province has the largest output and the best quality. However, there are serious varieties of schisandra in almost all planting bases of Schisandra chinensis, mainly reflected in fruit color, ear shape, yield, effective The differences in ingredient content, leaf texture and size, etc., have caused serious unevenness in the quality of Schisandra medicinal materials. At present, the propagation method used in the cultivation base of Schisandra chinensis is seed propagation, but this method will inevitably cause problems such as reproductive separation and degradation of agronomic traits. In the offspring obtained by seed propagation, there will be a phenomenon that the q...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 于继鸿
Owner LIAONING WANMU SCHISANDRA TECH IND PARK CO LTD
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