Method and device for measuring anaerobic ammonia oxidation rate and contribution rate of anaerobic ammonia oxidation for removing nitrogen in water/sediment system in situ
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An anaerobic ammonium oxidation and sediment technology, which can be used in measuring devices, instruments, scientific instruments, etc., and can solve problems such as the inability to meet the contribution of anaerobic ammonium removal.
Active Publication Date: 2015-06-17
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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The molecular biology methods currently used can no longer satisfy the research on the contribution of the anammox process to the nitrogen removal in nature. It is urgent to use biogeochemical methods such as isotope tracer to measure the rate of anammox, and then clarify its role in the natural nitrogen cycle. important role in
The currently used methods for determining the rate of anammox are limited to indoor culture experiments or semi-in-situ experiments where in-situ column samples are collected for indoor culture.
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Embodiment 1
[0053] Example 1. Device for in-situ determination of anaerobic ammonium oxidation rate and its contribution to nitrogen removal in Qilihai wetland water / sediment system in Tianjin
[0054] The schematic diagram of the device is shown in figure 1 As shown, it includes a stainless steel tube 11 with open ends, a rubber plug 10 matching the stainless steel tube 11, and accessories arranged through the rubber plug 10;
[0055] The rubber plug 10 is completely sealed with the stainless steel tube 11;
[0056] The accessories on the rubber stopper include pressure regulating device, stirring device and sampling device;
[0057] Among them, the stirring device includes a waterproof motor 6, a transmission shaft 7 and a stirrer 9 connected in sequence; the waterproof motor 6 is located at the center of the rubber plug 10 and penetrates the rubber plug 10; the transmission shaft 7 and the stirrer 9 are located in the tube 11;
[0058] The junction of the waterproof motor 6 and the r...
Embodiment 2
[0064] Example 2. In situ determination of the anammox rate in Tianjin Qilihai wetland water / sediment system and its contribution to nitrogen removal
[0065] 1) In Tianjin Qilihai wetland water and land ecotone area, select a research site with a suitable depth of overlying water, put stainless steel pipe 11 (inner diameter 6.2cm, length 60cm) into the sediment 40cm, after the stainless steel pipe 11 is open and stable for 1 day, add 1.7 mL, 200mM 15 NO 3 - Aqueous solution (abundance F N is 99.29%), so that the NO in the water body overlying the column 3 - -N concentration reaches 7.89mg L -1 . Then put the pressure regulating syringe (that is, the syringe without piston) 1 (20mL), waterproof motor 6 (model: MPM-060BS-12C-298I-3.039; specification: 4.0cm×2.0cm×1.5cm), sampling glass Tube 8 (inner diameter 0.2 cm, length 22 cm), silicone tube 4 (specification: 6 cm × 4 cm), three-way valve 2 and rubber stopper 10 of sampling syringe 3 (specification is No. 13, upper di...
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Abstract
The invention discloses a method and a device for measuring the anaerobic ammonia oxidation rate and the contribution rate of anaerobic ammonia oxidation for removing nitrogen in a water / sediment system in situ. The device comprises a pipe (11), a rubber plug (10) and accessories, wherein both ends of the pipe (11) are open, the rubber plug (10) is matched with the pipe (11), and the accessories are distributed on the rubber plug (10) in a penetrating mode; the rubber plug (10) is completely sealed with the pipe (11); the accessories on the rubber plug include a pressure regulating device, a stirring device and a sampling device. According to the method and the device, a 15N isotopic tracing method is utilized to in-situ culture and collect overlying water of homogeneous sediments within a certain space range of the water / sediment system, thereby further measuring soluble 29N2 and 30N2 to calculate the anaerobic ammonia oxidation rate and the contribution rate of the anaerobic ammonia oxidation for removing the nitrogen within a certain area of the water / sediment system. The method and the device can satisfy environmental conditions of simulating in-situ temperature, illumination, states of nutrient elements and the like, and typical samples are collected to be used for measuring the anaerobic ammonia oxidation rate and the contribution rate of the anaerobic ammonia oxidation for removing the nitrogen.
Description
technical field [0001] The invention relates to a method and a device for in situ measuring the anaerobic ammonia oxidation rate of a water / sediment system and its contribution rate to nitrogen removal. Background technique [0002] Anaerobic ammonium oxidation is one of the most shocking discoveries in the field of environmental science in the past decade, and related research results have been published in journals such as "Nature", because this process completely avoids N 2 The generation of O has therefore received extensive attention from the international community. Anaerobic ammonium oxidation is a process in which anammox bacteria (inorganic chemical energy autotrophic bacteria) directly oxidize ammonia nitrogen to produce nitrogen gas (NH 4 + +NO 2 - →N 2 +2H 2 O). Compared with traditional denitrification to produce N 2 Pathway (NO 3 - →NO 2 - →NO→N 2 O→N 2 ), Anammox is another important N-producing 2 way. [0003] The anaerobic ammonium oxidation ...
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 祝贵兵王朝旭尹澄清
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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