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Inorganic nano composite catalyst with peroxidase property and application of catalyst for performing catalytic decomposition on TMB thereof

A technology of composite catalyst and peroxidase, which is applied in the field of nanomaterial science and enzyme-like catalysis, can solve the problems of complex extraction process, high price, and inability to recycle and reuse, and achieve the effect of simple process and low input cost

Active Publication Date: 2013-09-11
JIANGSU CHINA NUCLEAR IND HUAWEI ENGDESIGN & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, peroxidase is easily denatured and inactivated by environmental changes (such as temperature increase, pH increase or decrease, etc.), so it cannot be used in harsher environmental conditions and can be decomposed by proteases; In addition, the extraction process of peroxidase is complex, expensive, storage conditions are harsh, and cannot be recycled, etc.

Method used

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  • Inorganic nano composite catalyst with peroxidase property and application of catalyst for performing catalytic decomposition on TMB thereof
  • Inorganic nano composite catalyst with peroxidase property and application of catalyst for performing catalytic decomposition on TMB thereof
  • Inorganic nano composite catalyst with peroxidase property and application of catalyst for performing catalytic decomposition on TMB thereof

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Experimental program
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preparation example Construction

[0016] Preparation of graphene oxide (Hummers S, Offeman R, J. Am. Chem. Soc., 1958, 80(6), 1339.): 1?g natural graphite, 1?g NaNO 3 Mix with 46?mL concentrated sulfuric acid (98%) and react in an ice-water bath for 4-5?h. Then 6?g KMnO 4 Add it into the mixture solution while raising the temperature to 35?°C and keep the reaction temperature. After the reaction is carried out for 2~3?h, add 90~95?mL H 2 O and the temperature was raised to 98°C. After two hours of reaction, 200?mL warm water and 20?mL 30% H 2 o 2 , and continue to react at room temperature for 1 ~ 2 h. Keep stirring throughout the reaction. The prepared product was filtered, washed with distilled water, and dried in an oven at 60° C. for 24˜48° h to obtain a yellow graphene oxide solid.

[0017] Fe 3 o 4 MnO 2 Preparation of Composite Nanomaterials (Z. Zhao, J. Liu, F. Cui, H. Feng and L. Zhang, J. Mater. Chem., 2012, 22, 9052.):

[0018] Place the beaker containing 100?mL ultrapure water in a water b...

Embodiment 1

[0020] After grinding the graphene oxide into pieces, weigh 0.1?g and add it into 100?mL PBS buffer solution for 1-2?h to obtain a uniform graphene oxide solution; take 0.2?g Fe 3 o 4 MnO 2 The composite nanoparticles were dispersed in 100?mL graphene oxide solution, ultrasonicated for 10 min, and the composite nanosheets were dispersed.

[0021] Peroxidase Activity Test of Inorganic Nanocomposite Particles

[0022] 1) At room temperature, the GO-Fe 3 o 4 MnO 2 After ultrasonic dispersion of inorganic composite nanoparticles, 20?μL of the solution was taken and added to 1.8?mL PBS buffer solution, and its absorbance in the range of 500-700?nm was measured as the background. Add 100 μL of chromogenic solution A and 200 μL of chromogenic solution B to the upper solution in sequence, and measure their absorption in the range of 500 to 700 μnm after reacting for 5 to 6 minutes.

[0023] 2) At room temperature, the Fe 3 o 4 MnO 2 After ultrasonic dispersion of inorganic comp...

Embodiment 2

[0028] After grinding the graphene oxide into pieces, weigh 0.4?g and add it into 100?mL PBS buffer solution for 1-2?h to obtain a uniform graphene oxide solution; take 0.8g Fe 3 o 4 MnO 2 Composite nanoparticles are dispersed in 100?mL graphene oxide solution, ultrasonicated for 10 minutes, and composite nanosheets are dispersed to obtain composite nanosheets with a side length of 40-70nm, which can effectively catalyze TMB, and the catalytic activity is better than that of graphene oxide or Fe 3 o 4 MnO 2 nanoparticles.

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Abstract

The invention discloses an inorganic nano composite catalyst with peroxidase property. The inorganic nano composite catalyst is characterized in that oxidized graphene and Fe3O4(at)MnO2 nano particles are mixed and are ultrasonically dispersed to obtain a composite nanosheet with the size being 40-70nm, wherein the concentration of the oxidized graphene is 1-4mg.mL<-1>, the concentration of Fe3O4(at)MnO2 is 2-8mg.mL<-1>, and the ratio of the concentration of the oxidized graphene to the concentration of Fe3O4(at)MnO2 is 1:2. The capacity of performing catalytic decomposition on TMB for the obtained composite catalyst is superior to that of the oxidized graphene or the Fe3O4(at)MnO2 nano particles, and the composite catalyst is wide in application range and can be applied to sewage treatment.

Description

technical field [0001] The invention belongs to the field of nanomaterial science and enzyme-like catalysis technology, and specifically relates to an inorganic nanocomposite catalyst with peroxidase properties, which can be used in normal temperature and near-neutral environment without special external energy (referring to current, voltage, UV radiation, etc.) to effectively catalyze hydrogen peroxide (H 2 o 2 ) decomposes to produce strong oxidizing hydroxyl radicals. Background technique [0002] With the rapid development of my country's industry, environmental pollution, especially water pollution, has become increasingly serious and has begun to restrict the development of my country's economy and society, becoming one of the most concerned issues in the 21st century. Among the many pollution sources that cause water pollution, highly toxic and refractory organic pollution sources account for a large proportion. At present, commonly used sewage treatment methods fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/889C02F1/72C02F101/38
Inventor 杜向前穆立文张岗巴换粉张立刚徐晓明乔风笙吴显斌徐枫刘维佳沈建锋李明波
Owner JIANGSU CHINA NUCLEAR IND HUAWEI ENGDESIGN & RES
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