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Pediococcus acidilactici viable preparation and preparation method thereof

A technology of Pediococcus lactis and live bacteria preparation, which is applied in the directions of biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve the problems that the density of viable bacteria of fermentation products is not high enough, the raw materials cannot be reused, the operation is complicated, etc. The effect of inhibiting bacterial contamination, high production cost and simple operation

Inactive Publication Date: 2013-09-11
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The previous fermentation methods of Pediococcus lactis mainly have the following problems and deficiencies: (1) Anaerobic culture tanks are used or the anaerobic environment is maintained by continuously feeding carbon dioxide or nitrogen. The raw materials cannot be reused, and the operation is more complicated and the cost is higher. High; (2) The post-processing of liquid fermentation products requires high-speed refrigerated centrifuges for centrifugation and low-temperature freeze-drying, the process is complicated, the cost is high, and the yield is low; (3) The viable bacteria density of fermentation products is not high enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 The technical process for preparing the live bacteria preparation of Pediococcus lactis comprises the following steps:

[0026] (1) Strain activation:

[0027] The present invention adopts Pediococcus lactis as the fermentation strain, which is purchased from the Guangdong Provincial Microbial Strain Preservation Management Center, and the preservation number is GIM1.263. , beef extract 10.0 g, yeast extract 5.0 g, diammonium hydrogen citrate 2.0 g, glucose 20.0 g, Tween 80 1.0 mL, sodium acetate 5.0 g, dipotassium hydrogen phosphate 2.0 g, magnesium sulfate 0.58 g, manganese sulfate 0.25 g , agar powder 15.0 g, distilled water 1 000 mL, pH 6.2~6.6, high-temperature and high-pressure sterilization) to draw a line on the solid slant, incubate at 37°C for about 16 hours, and the bacteria are generally activated 1 to 3 times;

[0028] (2) Preparation of primary seeds:

[0029] Pick one ring of activated Pediococcus lactis with an inoculation loop and ino...

Embodiment 2

[0036] Embodiment 2 is different from above-mentioned embodiment 1:

[0037] (1) Medium formula: corn flour 60, wheat flour 30, bean cake powder 10, glucose 10, yeast extract powder 1.5, peptone 3.0, the raw materials should be calculated in grams, and the corresponding parts in mL of distilled water should be 800~1000. 100°C, 30min high temperature and high pressure sterilization, when the temperature of the fermenter is lowered to 35~45°C, add 1.2g / 1000mL of inorganic salt base material, the composition of inorganic salt: MgSO 4· 7H 2 O 0.04%, MnSO 4 ·H 2 O 0.04%, in order to avoid the formation of precipitation, stir evenly during the addition process, then add liquid paraffin to a thickness of about 1cm;

[0038] (2) Temperature-controlled fermentation: The inoculation amount of Pediococcus lactis liquid seeds was 3%, stirred evenly, the initial pH was 6.6, the pH was controlled at 6.0-6.8, the fermentation temperature was controlled at 37-40°C, and the fermentation was...

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Abstract

The invention relates to a production method of a Pediococcus acidilactici viable preparation, particularly a production method of a Pediococcus acidilactici viable preparation by liquid anaerobic fermentation, belonging to the technical field of microorganisms. The method comprises the following steps: inoculating Pediococcus acidilactici used as a zymophyte strain into an optimized liquid nutrient medium, culturing at 37-40 DEG C for 16-18 hours, putting in a tank, determining the viable count, adsorbing the fermentation liquid with plant starch, drying, pulverizing, and packaging to obtain the stable-activity Pediococcus acidilactici preparation. The invention can implement high-density fermentation of Pediococcus acidilactici in a common fermentation tank, is simple to operate, has the advantages of stable effect and low energy consumption, does not use any centrifugal machine, freeze drier or the like, lowers the production cost, enhances the production efficiency, and thus, is easy to popularize and use.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a method for preparing a probiotic feed additive through anaerobic fermentation of Pediococcus lactis in a liquid medium. Background technique [0002] Pediococcus lactis ( Pediococcus acidilactici ) Gram-positive bacteria, non-motile, facultatively anaerobic. The cells are spherical and split alternately in two right-angled planes to form quadruples. Generally, the cells are in pairs, and solitary cells are rare, and they are not arranged in chains. Colonies on MRS medium are small and white. Growths along the puncture line of the agar are filamentous. Catalase negative, no cytochrome production. [0003] The physiological characteristics of Pediococcus lactis are as follows: produce acid, regulate the gastrointestinal flora, and maintain the intestinal microecological balance. It can antagonize pathogenic microorganisms in animals, competitively inhibit pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16C12N1/20C12R1/01
Inventor 李帅伟李本涛
Owner GUANGZHOU GLAM BIOTECH
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