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Tea source eurotium cristatum strain and application thereof

A technology of R. coronoides and strains, applied in fungi, pre-extraction tea treatment, microorganisms, etc., can solve the problems of moldy and scrapped Fu brick tea, long production cycle, unstable quality of Fu brick tea, etc.

Active Publication Date: 2013-09-04
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of natural fermentation to produce Fuzhuan brick tea, the production cycle is generally long, and a variety of bacteria are likely to be produced in the early stage of fermentation, resulting in unstable quality of Fuzhuan brick tea, and in severe cases, Fuzhuan brick tea may become moldy and scrapped

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 Preparation of bacterial strain of the present invention

[0016] Collect mature young shoots with one bud, four or five leaves of tea trees from Yueyang, Hunan. During the process of making Fuzhuan tea, when the bricks are pressed and formed to flower naturally for 3 days, weigh 25g and place them in a container filled with 225 mL of sterilized 0.1% peptone water. In the Erlenmeyer flask, fully shake for 5 minutes, after 10-fold serial dilution, take 0.1mL of each of the three dilutions of the bacterial suspension and spread it on the M40Y medium plate, incubate at 25°C for 7 days, observe the colony shape, and take a single colony to separate and purify , to obtain the inventive strain XU08.

Embodiment 2

[0017] Embodiment 2 The preparation of bacterial strain XU08 fermented liquid of the present invention

[0018] The thalli and buns of the bacterial strain XU08 activated on the M40Y slant medium were collected and inoculated into the M40Y liquid medium for cultivation. The inoculum amount was to add 1 g of thalli and buns per 100 mL of the M40Y liquid medium, and the culture temperature was 26 ° C. The stirring speed is 80r / min, and the cultivation time is 10 days.

Embodiment 3

[0019] Embodiment 3 Preparation of bacterial strain XU08 solid starter of the present invention

[0020] After collecting the thalli and steamed stuffed buns of the bacterial strain XU08 activated on the M40Y slant medium, inoculate them into the solid fermentation medium. Cultured for 5 days.

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PUM

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Abstract

The invention discloses a tea source eurotium cristatum strain which is named as eurotium cristatum XU08 and is preserved in the CGMCC (China General Microbiological Culture Collection Center) on March 13, 2012 with a preservation number of CGMCC No.5891. Researches on the morphological feature, the physiological property, the genetic stability and the growing conditions of the strain during the production of the Fuzhuan tea and industrial trial production of the strain prove that the strain is available as a special leavening agent for the production of the Fuzhuan tea, so that the production cycle can be shortened and the product quality can be improved, and therefore, the strain is very suitable for modern production needs of the Fuzhuan tea.

Description

technical field [0001] The invention belongs to the field of microbe application and food processing technology, and in particular relates to a tea-derived Saccharomyces coronis strain and its application in the production and fermentation of Fuzhuan tea. technical background [0002] Fuzhuan tea is a fully fermented tea, which is the most complex and unique dark tea product among all teas. On the surface of the tea leaves of Fuzhuan brick tea, there are generally small golden spots commonly known as "golden flowers", which is an important feature that distinguishes Fuzhuan brick tea from other brick teas. "Golden flower" is the golden-yellow closure shell produced by the coronoid fungus in Fuzhuan brick tea. The amount of "golden flower" is an important indicator of the quality of Fuzhuan brick tea. [0003] Coronis can produce various extracellular enzymes (such as polyphenol oxidase, pectinase, cellulase, protease, etc.), as an effective biochemical power, catalyzing the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23F3/10C12R1/645
Inventor 李宗军李珂许爱清刘仲华
Owner HUNAN AGRICULTURAL UNIV
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