Edwardsiella ictaluri colloidal gold immunochromatographic strip and preparation method thereof
A technology of immunochromatography test paper and colloidal gold, which is applied in the direction of analysis materials, measuring devices, instruments, etc., can solve the problem of no application of colloidal gold immunochromatography test strips, and achieve the effect of rapid detection, easy operation and high sensitivity
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[0029] Edwardsiella colloidal gold immunochromatography test strip structure of the present invention is identical with prior art, has base plate 5, on base plate 5, is stuck with sample pad 4, gold standard pad 3, The nitrocellulose membrane 2 and the water-absorbing pad 1 overlap each other. The difference from the prior art is that the colloidal gold-labeled Edwardsiella monoclonal antibody is sprayed on the gold label pad 3, and the detection line a coated with the Edwardsiella polyclonal antibody is on the nitrocellulose membrane 2, and there is a goat anti-mouse The quality control line b composed of IgG, the Edwardsiella monoclonal antibody is prepared by immunizing mice with hybridoma cells with the preservation number CGMCC No. 7305, and purifying the ascites of the mice.
[0030] The hybridoma cell secreting the anti-Edwardia monoclonal antibody is prepared as follows:
[0031] 1. Preparation of bacterial liquid
[0032] Edwardsiella catarrhalis (ATCC 33202) was c...
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