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Rapid urokinase absorption bag

An adsorption bag, urokinase technology, applied in the field of separation and purification, can solve the problems of inconvenient collection and transportation of raw materials, difficult to achieve large-scale production, and the need for pressurized equipment, reducing the collection of raw urine, facilitating large-scale industrial production, cost saving effect

Active Publication Date: 2013-08-28
NANJING NANDA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods all require long-term soaking and stirring of the adsorbent material in the raw urine, so the collection and transportation of a large amount of raw urine cannot be avoided by using these methods, so not only the cost is high, but also the collection and transportation of raw materials are inconvenient
[0006] CN88103076.7 discloses an alkali-treated polyacrylonitrile ultrafiltration membrane to concentrate raw urine so that urokinase is adsorbed on the membrane. This method can obtain relatively high-purity urokinase. The process requires pressurized equipment, and it is difficult to achieve large-scale production. At the same time, it is unavoidable to collect and transport a large amount of raw urine.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Take 50g of absorbent cotton, add 46g of sodium hydroxide, 600ml of water, and stir at 37°C for 2 hours. Then, 60 g of monochloroacetic acid was added and stirred at 75°C for 4 hours. After the reaction product was separated, it was repeatedly rinsed with pure water to neutrality, then rinsed with absolute ethanol for 3 times, and dried in vacuum to obtain modified absorbent cotton fibers.

[0035] (2) Take the raw silica gel, soak it in 300ml 6mol / L hydrochloric acid solution for 4 hours, wash until there is no chloride ion, and dry it at 130°C for 2 hours. Activate 50g of dried silica gel in a muffle furnace at 250°C for 4 hours, add 200ml of toluene and 20g of phenyltrichlorosilane, reflux and stir for 24 hours, filter after the reaction, wash with toluene, acetone and methanol in sequence, and dry to obtain Precursor. Take 50g of precursor silica gel and put it into a beaker, add 200ml of 98% concentrated sulfuric acid, sulfonate at 100°C for 2 hours, filter th...

Embodiment 2

[0038](1) Take 30g of absorbent cotton, add 21.6g of sodium hydroxide, 720ml of water, and stir at 30°C for 3 hours. Then, 108 g of monochloroacetic acid was added and stirred at 70°C for 5 hours. After the reaction product was separated, it was repeatedly rinsed with pure water to neutrality, then rinsed with absolute ethanol for 3 times, and dried in vacuum to obtain modified absorbent cotton fibers.

[0039] (2) Take the raw silica gel, soak it in 500ml 4mol / L hydrochloric acid solution for 5 hours, wash until there is no chloride ion, and dry it at 120°C for 3 hours. Activate 50g of dried silica gel in a muffle furnace at 200°C for 6 hours, add 100ml of toluene and 35g of phenyltrichlorosilane, reflux and stir for 10 hours, filter after the reaction, wash with toluene, acetone and methanol in sequence, and dry to obtain Precursor. Take 50g of the precursor silica gel and put it into a beaker, add 500ml of 98% concentrated sulfuric acid, sulfonate it at 90°C for 5 hours, f...

Embodiment 3

[0042] (1) Take 50g of absorbent cotton, add 200g of sodium hydroxide, 400ml of water, and stir at 40°C for 2 hours. Then add 60 g of monochloroacetic acid and react at 80° C. for 3 hours. After the reaction product is separated, it is repeatedly washed with pure water to neutrality, then washed with absolute ethanol for 3 times, and vacuum-dried to obtain the modified absorbent cotton fiber.

[0043] (2) Take the raw silica gel, soak it in 100ml of 8mol / L hydrochloric acid solution for 3 hours, wash until there is no chloride ion, and dry at 140°C for 1 hour. Activate 50g of dried silica gel in a muffle furnace at 300°C for 2 hours, add 500ml of toluene and 50g of phenyltrichlorosilane, reflux and stir for 40 hours, filter after the reaction, wash with toluene, acetone and methanol in sequence, and dry to obtain Precursor. Take 50g of the precursor silica gel and put it into a beaker, add 350ml of 98% concentrated sulfuric acid, sulfonate it at 110°C for 2 hours, filter thro...

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Abstract

The present invention provides a rapid urokinase absorption bag, which comprises a mutton gauze layer, a modified cellulose layer, and a modified resin layer or a modified silica gel layer, wherein the mutton gauze layer, the modified cellulose layer, and the modified resin layer or the modified silica gel layer are sequentially arranged from the outside to the inside. The rapid urokinase absorption bag has the following characteristics that: a structure is simple; use is convenient; combination of the modified cellulose layer and the modified resin layer or the modified silica gel layer is adopted, such that the prepared absorption bag can timely and rapidly absorb urokinase in a toilet urinal so as to reduce steps of initial urine collection, transportation and the like in the traditional method, substantially simplify steps and workload of urokinase crude product extraction, provide characteristics of convenience and rapidness, and substantially save cost. In addition, large-scale industrial production of the rapid urokinase absorption bag is easily achieved.

Description

technical field [0001] The invention belongs to the field of separation and purification, in particular to a device for rapid adsorption of urokinase in raw urine. Background technique [0002] Urokinase is a proteolytic enzyme present in urine, its main function is to activate plasminogen (plasminogen) to become active plasmin (plasmin), thereby making fibrin The clot (thrombus) dissolves. Urokinase has a good curative effect on the treatment of cerebral thrombosis, myocardial infarction and other thrombotic diseases, and is widely used in the treatment of fresh thrombotic occlusive diseases, pulmonary embolism, acute cerebral thrombosis, cerebral vascular embolism and central retinal vein thrombosis. Human-derived urokinase mainly includes natural high-molecular-weight urokinase (54.7KD) and low-molecular-weight urokinase (33KD). [0003] The clinical effect of high-molecular-weight urokinase on dissolving thrombus is about 3 times higher than that of low-molecular-weigh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/28B01D15/00C08B11/12C08F20/10C08F8/36C08G77/392
Inventor 邹杰张弢方银杏孙涛
Owner NANJING NANDA PHARMA
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