Method for catalyzing inulin fructose and cassava glucose to produce high-concentration sorbitol and gluconic acid by using immobilized movement fermented monas

Abstract

The invention relates to a method for simultaneously catalyzing inulin fructose and cassava glucose to produce high-concentration sorbitol and gluconic acid by using immobilized recombination movement fermented monas. The method comprises specific steps that: (1) a high-concentration equimolar inulin fructose and cassava glucose mixed hydrolysate is prepared with an enzymatic method; (2) cell immobilization of recombination movement fermented monas is carried out; and (3) the immobilized recombination movement fermented monas is used for simultaneously catalyzing fructose and glucose, such that a mixed product comprising high-concentration sorbitol and gluconic acid is obtained. The method provided by the invention has the advantages that: one glucoamylase is used for synchronously hydrolyzing inulin and cassava starch, such that equimolar fructose and glucose are produced; raw material cost is low, enzyme application amount is reduced, and production process is simplified; immobilized cells are circulated in catalyzing fructose and glucose to produce sorbitol and gluconic acid, such that catalyst dose is reduced, and continuous production of cell catalyst is avoided; the high-concentration sorbitol and gluconic acid mixed liquid is obtained, such that subsequent product separation cost is reduced.

Description

【Technical field】 [0001] The invention relates to the field of bio-manufacturing and bio-refining of bio-based chemicals, specifically, using inulin and tapioca starch as raw materials, using recombinant Zymomonas mobilis to catalyze equimolar concentration of inulin fructose and tapioca starch to produce high Method of Concentration Sorbitol and Gluconic Acid. 【Background technique】 [0002] Sorbitol is a widely used chemical raw material, mainly used in the production and preparation of sweeteners, wetting agents, conditioners, softeners, etc. The current industrial production technology of sorbitol is to use 50% D-glucose syrup catalytic hydrogenation process, but this chemical method to produce sorbitol is a process with high energy consumption, high recovery cost and environmental pollution. The biological method to produce sorbitol is mainly to use the glucose-fructose oxidoreductase in the periplasmic space of Zymomonas mobilis to convert fructose and glucose to gene...

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Problems solved by technology

However, in these studies, the cells were first treated with toluene, cetyltrimethylammonium bromide or metal ions for catalytic experiments, and it was believed that fresh untreated cells would have a large amount of sorbitol and gluconic acid in the direct catalytic production of sorbitol and gluconic acid. The by-product ethanol is produced and cannot be recycled repeatedly, which will affect the production efficiency and production cost of the whole process

At the same time, these studies obtained lower concentrations of sorbitol and gluconic acid, which increased the cost of subsequent product isolation

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