Extraction method of acridine coumarin alkali F

An extraction method and technology for coumarin, applied in directions such as organic chemistry, can solve the problems such as the patent report on the extraction and purification method of acridine coumarin base that has not yet been seen, and achieve high economic benefits and academic value, short time consumption, and easy industrialization. zoom-in effect

Inactive Publication Date: 2013-08-21
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there is no patent report on the extraction and purification method of acridine coumarin base F in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 1 kg of the root of grapefruit hybrid tangerine, crush it into a coarse powder, feed it from the feed inlet of the head of the continuous countercurrent ultrasonic extraction equipment, add 10 times of 95% ethanol solution from the solvent inlet at the tail of the continuous countercurrent ultrasonic extraction equipment, and carry out at 55 ° C. Continuous countercurrent ultrasonic extraction for 30 minutes, the ultrasonic frequency is 80KHz, to obtain the extract, the extract is decompressed to recover the reagent to obtain the extract, add alumina (120 mesh) to the extract, mix well, dry and pack the column, use 7 times the column volume Chloroform-acetone (1:1) mixed solution was eluted, TLC detection was performed, and acridine coumarin base F fractions were collected, concentrated and dried to obtain acridine coumarin base F crude product. With acetonitrile-0.2% ethylenediamine aqueous solution as the mobile phase, the volume ratio is 33:67, the chromatographi...

Embodiment 2

[0024] Take 1 kg of the root of grapefruit hybrid orange, crush it into coarse powder, feed it from the feed inlet of the head of the continuous countercurrent ultrasonic extraction equipment, add 10 times of 85% ethanol solution from the solvent inlet at the tail of the continuous countercurrent ultrasonic extraction equipment, and carry out at 45 ° C. Continuous countercurrent ultrasonic extraction for 100min, the ultrasonic frequency is 60KHz, to obtain the extract, the extract is decompressed to recover the reagent to obtain the extract, add alumina (80 mesh) to mix well, dry and pack the column, and use 4 times the column volume of chloroform-acetone ( 1:1) mixed solution for elution, TLC detection, collecting acridine coumarin base F fraction, concentrating and drying to obtain acridine coumarin base F crude product. Using acetonitrile-0.2% ethylenediamine aqueous solution as the mobile phase, the volume ratio is 33:67, the chromatographic column uses the C8 bonded phase ...

Embodiment 3

[0026] Take 10 kg of the root of grapefruit hybrid orange, crush it into coarse powder, feed it from the feed inlet of the head of the continuous countercurrent ultrasonic extraction equipment, add 6 times of 90% ethanol solution from the solvent inlet at the tail of the continuous countercurrent ultrasonic extraction equipment, and carry out at 35 ° C. Continuous countercurrent ultrasonic extraction for 50min, the ultrasonic frequency is 50KHz, to obtain the extract, the extract is decompressed to recover the reagent to obtain the extract, add alumina (100 mesh) and mix well, dry and pack the column, and use 6 times the column volume of chloroform-acetone ( 1:1) mixed solution for elution, TLC detection, collecting acridine coumarin base F fraction, concentrating and drying to obtain acridine coumarin base F crude product. With acetonitrile-0.2% ethylenediamine aqueous solution as the mobile phase, the volume ratio is 33:67, the chromatographic column uses the C8 bonded phase ...

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Abstract

The invention discloses an extraction method of acridine coumarin alkali F. The extraction method comprises the following steps: (1) taking coarse root powder of grapefruit hybrid oranges and 85-95% of ethanol solution to carry out continuous counter-current ultrasonic extraction so as to obtain an extracting solution; (2) concentrating the extracting solution, and then carrying out alumina column chromatography on the extracting solution; (3) carrying out preparative liquid chromatographic separation on the obtained object; and (4) carrying out chloroform-methanol recrystallization on the obtained product so as to obtain a high-purity acridine coumarin alkali F crystal product. The method disclosed by the invention is simple and high in yield, can obtain high-purity products, and has high economic and academic values.

Description

technical field [0001] The invention belongs to the field of natural plant extraction, and in particular relates to an extraction method of acridine coumarin base F. Background technique [0002] Acridine coumarin base F (Acrimarine F), molecular formula C 31 h 29 NO 8 , molecular weight 543.57. Acridine coumarin F is an acridine alkaloid isolated from the root of Citrus paradisi×Citrus tangerine. Experimental studies have shown that acridine coumarin base F can inhibit virus activation. It has 100% inhibitory effect on Epstein-Barr virus (EBV) at 500mol ratio / TPA concentration, and 66.8% inhibitory activity at 10mol ratio / TPA. [0003] Existing reports of extracting acridine coumarin base F are rare, and there is no sale of acridine coumarin base F reference substance on the market, so it is very difficult to provide an extraction method of acridine coumarin base F If necessary, it can provide support for its follow-up research. [0004] At present, there is no paten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D405/06
Inventor 苏刘花万冬梅
Owner NANJING ZELANG AGRI DEV
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