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Method for preparing trifolium pretense oral liquid

A technology of red clover and oral liquid, which is applied in the field of preparation of red clover oral liquid, can solve the problems of affecting drug efficacy and utilization, low bioavailability, poor water solubility, etc., and achieve breast cancer prevention, simple technology and low cost Effect

Active Publication Date: 2013-08-21
中农牧生物科技集团(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Red clover isoflavones are affected by intermolecular hydrogen bonds, making them tightly packed, so their water solubility is poor, and it is not easy to absorb in the body, resulting in low bioavailability, which affects its efficacy and utilization

Method used

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  • Method for preparing trifolium pretense oral liquid
  • Method for preparing trifolium pretense oral liquid
  • Method for preparing trifolium pretense oral liquid

Examples

Experimental program
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preparation example Construction

[0035] Preparation of standard curve of biochanin A

[0036] With 70% ethanol by mass percentage, the chickpein was prepared according to 2.0, 4.0, 6.0, 8.0, 10.0, 12.0, 14.0 μg / mL, and 70% ethanol by mass percentage was used as the control. In a quartz cuvette, use a spectrophotometer to measure the absorbance at 263 nm, and draw a standard curve.

Embodiment 1

[0038] A preparation method of red clover oral liquid comprises the following steps.

[0039] The first step, preparation of red clover extract

[0040] Configure alcohol with a mass percentage concentration of 70% as an organic solvent, crush the red clover with a hammer mill, soak the red clover in the organic solvent for 48 h with a mass-volume ratio of 1:10 g / L, filter, reduce The filtrate was concentrated under pressure to obtain a concentrated solution, and dried at 60°C to constant weight to obtain a red clover extract.

[0041] The second step, water-soluble modification of red clover extract

[0042] pH and temperature for metal ion chelation:

[0043] It is found through experiments that increasing the pH and temperature can increase the water solubility of isoflavones. However, if the pH and temperature are too high, the effect is not significant. Therefore, after a single-factor experiment, a two-factor five-level orthogonal experiment was designed with pH 9-13...

Embodiment 2

[0083] A preparation method of red clover oral liquid, comprising the steps of:

[0084] The first step, preparation of red clover extract

[0085] Configure alcohol with a mass percentage concentration of 70% as an organic solvent, crush the red clover with a hammer mill, soak the red clover in the organic solvent for 48 h with a mass-volume ratio of 1:10 g / L, filter, reduce Concentrate the filtrate under pressure to obtain a concentrated solution, dry it at 60°C to constant weight, and obtain the red clover extract;

[0086] The second step, water-soluble modification of red clover extract

[0087] The dried red clover extract is dissolved in an alcohol solution with a mass percentage concentration of 70% to saturation, and the KOH solution is used as a modification solution to modify the red clover extract to adjust the pH of the saturated red clover extract solution The value is 9-13, heated in a water bath at a temperature of 30-70°C for 4 h, concentrated under reduced ...

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Abstract

The invention discloses a method for preparing trifolium pretense oral liquid. The method comprises the steps of firstly, preparing 70% alcohol as an organic solvent, crushing the trifolium pretense, soaking the trifolium pretense into the organic solvent for 48 hours according to the quality-volume ratio (g / L) of 1:10; filtering and concentrating filtrate to obtain concentrated solution by reduced pressure; drying to constant weight at 60 DEG C to obtain trifolium pretense extract; dissolving the dried trifolium pretense extract into an alcohol solution until the solution is saturated; modifying by a NaOH or KOH solution, adjusting the pH value to 9-13, heating for 4 hours in a water bath kettle of which the temperature is 30-70 DEG C; concentrating by reduced pressure, and drying to constant weight at 60 DEG C; then taking distilled water, and adding the modified trifolium pretense extract to 4 mg / ml of trifolium pretense isoflavone in the solution under a continuous stirring condition; adding strawberry essence and xylitol, adjusting the pH value to 5-7, and evenly mixing to obtain the trifolium pretense oral liquid with high isoflavone content and good taste.

Description

technical field [0001] The invention relates to a preparation method of red clover oral liquid, which belongs to the technical field of functional food. technical background [0002] Red clover (Trifolium pratense L.) is a leguminous perennial herb, also known as red clover. It is an excellent leguminous forage in the south of the Yangtze River Basin in my country. At present, it has been introduced and cultivated in many areas in China. Red clover contains flavonoids, proteins, amino acids, sugars and vitamins. Among them, isoflavones have attracted special attention because of their phytoestrogen-like effects and the anticancer effects of flavonoids. [0003] Isoflavones are biologically active substances that belong to flavonoids and widely exist in the plant kingdom. There are more than 700 known isoflavones in plants, and almost all of them are distributed in the legume plant Papilionoideae. In some genera, such as soybean, red clover, kudzu, and alfalfa. Among them,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L33/00
Inventor 邱龙新黄毅萱苏伟娇王天喜林发新翁梦婷
Owner 中农牧生物科技集团(厦门)有限公司
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