Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving enzymolysis saccharification efficiency of lignocellulose

A lignocellulosic enzyme and lignocellulose technology, which is applied in the field of improving lignocellulose enzymatic saccharification efficiency, can solve the problems of pretreatment and enzymatic hydrolysis efficiency reduction, low-efficiency storage technology, low technical efficiency, etc., and achieve enzymatic saccharification The effect of improving the efficiency, reducing the degradation products, and maintaining the flexibility and porosity

Inactive Publication Date: 2013-08-14
TIANJIN UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for improving the efficiency of enzymatic hydrolysis and saccharification of lignocellulose in view of the problems of low technical efficiency and high cost of existing storage methods, and the low efficiency of storage technology will lead to reduced pretreatment and enzymatic hydrolysis efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for improving lignocellulose enzymatic saccharification efficiency, comprising the steps of:

[0027] (1) Dry the newly harvested corn stalks to a moisture content of 60%, and then crush them with a pulverizer to make the length 2.8 ± 0.2 cm, and seal them in a sealed bag with a dry weight density of lignocellulosic raw materials of 60 g / L. 9 months storage

[0028] (2) Put the material obtained in step (1) into the reaction tank of the steam explosion reactor, close the material inlet valve, feed steam to heat until the reaction temperature is 210°C, keep it for 5 minutes, release the pressure instantly, and explode the material to In the receiving tank, cool for 10 minutes, open the material outlet valve, collect the solid-liquid mixture, separate the solid and liquid, wash the solid with water, dry at 45°C, collect the liquid and determine the composition, the recovery rate of steam explosion pretreatment dextran is 100% , the recovery rate of xylan was 72%...

Embodiment 2

[0031] A method for improving lignocellulose enzymatic saccharification efficiency, comprising the steps of:

[0032] (1) Dry the freshly harvested sorghum stalks to a moisture content of 35%, and then crush them with a grinder so that the length is 3.8±0.2cm, and pack them in a sealed tank with a dry weight density of lignocellulosic raw materials of 50g / L. 3 months storage;

[0033] (2) Put the material obtained in step (1) into the reaction tank of the intermittent steam explosion reactor, close the material inlet valve, and heat it with steam until the reaction temperature is 170°C, keep it for 10 minutes, release the pressure instantly, and put the material Explode into the receiving tank, cool for 10 minutes, open the material outlet valve, collect the solid-liquid mixture, separate the solid and liquid, wash the solid with water, dry at 45°C, collect the liquid and determine the composition, the recovery of steam explosion pretreatment dextran is 98%, the recovery rate...

Embodiment 3

[0036] A method for improving lignocellulose enzymatic saccharification efficiency, comprising the steps of:

[0037] (1) Dry the newly harvested wheat straw to a moisture content of 30%, and then crush it with a pulverizer so that the length is 1.2±0.2cm, and pack it in a sealed cellar with a dry weight of lignocellulosic raw material of 120g / L. Store for 1 month;

[0038] (2) Add the material obtained in step (1) into the reaction tank of the steam explosion reactor, close the material inlet valve, feed steam to heat until the reaction temperature is 190°C, maintain it for 8 minutes, release the pressure instantly, and explode the material to In the receiving tank, cool for 10 minutes, open the material outlet valve, collect the solid-liquid mixture, separate the solid and liquid, wash the solid with water, dry at 45°C, collect the liquid and determine the composition, the recovery rate of steam explosion pretreatment dextran is 90% , the recovery rate of xylan was 62%;

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for improving the enzymolysis saccharification efficiency of lignocellulose, which comprises the following steps of: (1) crushing a lignocellulose raw material with a water mass of 30-75%, packaging the crushed material in a sealing device, and storing the crushed material; (2) adding the material obtained in the step (1) into a steam explosion reactor, heating the material by using steam, instantaneously releasing pressure so as to obtain a solid-liquid mixture, carrying out e solid-liquid separation on the solid-liquid mixture, carrying out washing and drying on the obtained solid; and (3) adding cellulase and water into the dried matter, adjusting the pH value of the obtained product to be 4.5-5.5, carrying out enzymolysis on the obtained product, and carrying out solid-liquid separation on the obtained product again so as to obtain a liquid rich in glucose and xylose. According to the method disclosed by the invention, the loss of biomass can be reduced, and the flexibility and porosity of a biomass structure can be maintained; the recovery rate of sugar is high, and degradation products are less; and the enzymolysis saccharification rate is significantly increased.

Description

technical field [0001] The invention relates to the technical field of biomass new energy, in particular to a method for improving the enzymatic hydrolysis and saccharification efficiency of lignocellulose. Background technique [0002] Today's world urgently needs a new energy to replace the increasingly depleted fossil energy, so as to reduce the economic, security and environmental pressure caused by fossil energy. Bioethanol produced from lignocellulose is considered to be a beneficial supplement to fossil energy. It has the advantages of low-carbon environmental protection and abundant resources. It is beneficial to the realization of national economic growth, energy security and environmental protection. [0003] The lignocellulosic ethanol process mainly includes unit operations such as planting and harvesting of raw materials, storage and crushing, pretreatment, enzymatic hydrolysis, fermentation and purification. The storage of raw materials will not only affect th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 元英进刘志华秦磊李炳志
Owner TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com