Heteroauxin-producing Arthrobacter pascens strain with fluoranthene degradation capacity and application thereof

A technology of indole acetic acid and Arthrobacter, applied in the field of microorganisms

Active Publication Date: 2014-08-27
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the plant growth-promoting bacteria have not been domesticated into strains that can effectively improve the stress resistance of plants. Therefore, it has become a hot spot to isolate plant growth-promoting bacteria that can effectively improve various stress resistance of plants from plant rhizosphere soil.

Method used

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  • Heteroauxin-producing Arthrobacter pascens strain with fluoranthene degradation capacity and application thereof
  • Heteroauxin-producing Arthrobacter pascens strain with fluoranthene degradation capacity and application thereof
  • Heteroauxin-producing Arthrobacter pascens strain with fluoranthene degradation capacity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] First prepare the following three media.

[0031] LB medium: peptone 10g, yeast extract 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH7.0-7.2, sterilized at 121°C for 20min.

[0032] LB liquid medium: without agar, other conditions are the same as above.

[0033] Inorganic phosphorus bacteria medium (PKO medium): 5g tricalcium phosphate, 10g glucose, 0.5g ammonium sulfate, 0.3g sodium chloride, 0.3g magnesium sulfate heptahydrate, 0.3g potassium chloride, 0.03g manganese sulfate, heptahydrate Ferrous sulfate 0.03g, pH7.0 agar 20g, distilled water 1000ml, pH7.0~7.2. Sterilize at 121°C for 20min.

[0034] Inorganic salt medium: ammonium sulfate 2.0g; sodium dihydrogen phosphate 0.5g; dipotassium hydrogen phosphate 0.5g; magnesium sulfate heptahydrate 0.2g; calcium chloride dihydrate 0.1g, distilled water 1000mL, pH7.0, sterilized at 121℃ , 20min.

[0035] Sieve the rhizosphere soil collected from the foot of Zijin Mountain in Nanjing, weigh 10g and place...

Embodiment 2

[0043] Aerobic test

[0044] Pour the sterilized LB culture medium into 3 sterilized test tubes, at about 2 / 3, on the aseptic operating table, pick up the ZZ21 (CGMCC No.7325) cultured on the slant with an inoculation needle, and puncture Inoculate into the above medium (must be pierced to the bottom of the tube). Cultivate at 30°C, and observe the results in 3 days to 7 days respectively. Those that grow on the surface of the agar column are aerobic bacteria, and those that grow along the puncture line are anaerobic or facultative anaerobic bacteria.

[0045] Determination of catalase

[0046] Put 1 drop of 3% H on a clean slide 2 o 2 , take 1 ring of 18-24h LB slant culture, in H 2 o 2 Smear in the middle, if bubbles are produced, it is positive, otherwise it is negative.

[0047] Methyl red test (M.R test)

[0048] a. Culture medium and reagents: 5g peptone, 5g glucose, 5g sodium chloride, 1000mL distilled water, adjust the pH to 7.0-7.2, divide into test tubes, eac...

Embodiment 3

[0069] In order to further verify the indole acetic acid production ability and optimal conditions of Arthrobacter halotolerant Arthrobacter ZZ21 (CGMCC No.7325) obtained in Example 1, the following is to explore the indole acetic acid production capacity for different pH, liquid volume, different carbon sources, and different nitrogen sources. Effect on acetic acid production.

[0070] Put 15ml, 30ml, 45ml, 60ml, 75ml, 90ml of LB liquid medium containing L-tryptophan (200mg / L) into a 150ml Erlenmeyer flask, and inoculate at a logarithmic After ZZ21 in the growth period, place at 30°C, 180r·min -1 Cultivate on a shaking table for 24 hours, and measure the amount of IAA produced by a quantitative method. The result is as image 3 As shown, due to the facultative aerobic metabolism of the strain ZZ21 (CGMCC No.7325), the aeration rate affects the efficiency of the strain producing IAA. When the amount of liquid is filled, the amount of IAA produced is the largest.

[0071] A...

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Abstract

The invention discloses a heteroauxin-producing Arthrobacter pascens strain with fluoranthene degradation capacity and application thereof, belonging to the field of microorganisms. The Arthrobacter pascens ZZ21 is collected at China General Microbiological Culture Collection Center on March 15th, 2013; the class name is Arthrobacter pascens; and the collection number is CGMCC No.7325. The strain can product heteroauxin at high yield, can grow regardless of salt and alkali, can grow by using insoluble phosphate as a phosphorus source, and has certain degradation effect on polycyclic aromatic hydrocarbon fluoranthene.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to an indoleacetic acid-producing Arthrobacter halotolerant strain with fluoranthene degradation ability and application thereof. Background technique [0002] Many microorganisms in the soil can dissolve insoluble phosphorus, promote the absorption of phosphorus by plants, increase crop yield and improve crop quality, and use phosphorus-dissolving microorganisms as biological fertilizers, which can not only improve the utilization efficiency of phosphorus in the soil, save fertilizer and increase production, but also It can improve soil structure and increase soil organic matter content. Plant Growth Promoting Bacteria (PGPB) is defined as bacteria living freely in the soil, rhizosphere, root surface, and phyllosphere that are beneficial to plant growth under certain conditions. These bacteria are able to fix nitrogen, dissolve phosphorus and iron, and produce plant hormones such as au...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C05G3/00C12R1/06A62D101/20
Inventor 徐莉张振李辉信陈雄李伟明李方卉焦加国刘满强陈小云胡锋
Owner NANJING AGRICULTURAL UNIVERSITY
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