Induction method of nitraria loose embryonic callus

Abstract

The invention relates to an induction method of nitraria loose embryonic callus. The method comprises the following steps of: (1) scissoring a stem between two blades of a nitraria aseptic seedling, and inoculating the stem to a culture medium for carrying out induction of loose callus; (2) after 2-3 weeks, inoculating the induced loose nitraria callus to the culture medium for carrying out induction of embryonic callus; in the process of inducing the loose callus to the embryonic callus, carrying out successive transfer culture once per 5-8 days; and in the process of successive transfer culture, transferring callus blocks; and (3) carrying out successive transfer culture for 4-5 times so that the loose nitraria callus is changed to relatively hard callus, the color gradually changes from transparent white to milk white, yellow and finally peak green, and at the moment, viewing from the organizational structure, the callus is of a loose structure formed by many spheroids. When the callus with the characteristics occurs, the induction of the nitraria embryonic callus is finished. According to the method, by inducing the nitraria embryonic callus, an induction system of the standard nitraria loose embryonic callus is established.

Description

Technical field [0001] The invention belongs to the fields of agriculture and plant breeding, and particularly relates to a method for inducing loose embryogenic callus of Nitraria tangutorum. Background technique [0002] Nitraria L., shrubs, Zygophyllaceae, there are 12 species in the world, 8 species in my country, mainly distributed in arid desert areas, and its fruits are rich in vitamins and amino acids, and have high medicinal properties And edible value. According to the survey, Nitraria tangutorum has male sterility, and the interspecific hybridization is chaotic, and the differentiation is serious. There are big differences in the size, color and taste of the fruit. Therefore, the research on the breeding technology of Nitraria in vitro has very important practical significance for the breeding and resource development of wild Nitraria resources. [0003] Because Nitraria tangutorum is restricted by the genetic resources of this species, some new excellent traits cannot ...

AI Technical Summary

Problems solved by technology

[0003] Because Nitraria is limited by the genetic resources of this species, some new excellent traits cannot be produced, and the efficiency of general mutation breeding is extremely low, so it is difficult to induce new excellent traits, although gene transformation technology can introduce target genes into Nitraria genome, but because transgenic plants are difficult to release in the environment, this technology only stays in the laboratory stage

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