Stirring reactor adopting film tube aeration
A stirred reactor and membrane tube technology, which is applied to special-purpose bioreactor/fermenter, bioreactor/fermenter combination, biochemical instrument, etc., can solve the problem of unfavorable aerobic microbial fermentation and low volume mass transfer coefficient , limit the growth of bacteria and other problems, and achieve the effect of good control, large quantity, and convenient disassembly and maintenance
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Embodiment 1
[0018] figure 1 It is a structural schematic diagram of the present invention, figure 2 yes figure 1 The top view of the A-A direction in the middle, combined with figure 1 , figure 2 As shown, the stirred reactor using membrane tube aeration includes a stirred reactor 1, and a plate-fin heat exchanger 2 with a plurality of fan-shaped plate-fin heat exchangers is arranged in the stirred reactor 1, and the fan-shaped Plate-fin heat exchangers are used to exchange heat. The upper end of the plate-fin heat exchanger 2 has a steam inlet and cooling water outlet flange 3, and the lower end has a steam condensate outlet and a cooling water inlet flange 7, wherein the steam inlet and cooling water outlet flange 3 pass through the ring pipe 4 The fan-shaped plate-fin heat exchangers are connected as a whole, and the steam condensate outlet and the cooling water inlet flange 7 connect the fan-shaped plate-fin heat exchangers as a whole through the ring pipe 26 to facilitate the f...
Embodiment 2
[0022] This example is used to illustrate the use of 10 m 3 The method for fermenting and producing L-lysine in a reactor using a membrane tube aeration method.
[0023] First, the culture medium is sterilized, and the stirring motor is turned on at a speed of 100 r / min, so that the fermentation broth is evenly heated. When the temperature rises above 95 degrees, the stirring can be stopped. Carry out the sterilization of the air filter and the air pipeline again, and slightly open the filter and the drain valve to ensure the sterilization of the air pipeline. Finally, the outlet, sampling valve, steam valve and outlet valve are slightly opened to ensure that the pipeline is sterilized.
[0024] Fermentation culture and results: Escherichia coli producing L-lysine (purchased from CGMCC, strain preservation number CGMCC1.0281) was inserted into a 5 m 3 10 m of fermentation medium 3 In a stirred reactor with membrane tube aeration (1L fermentation medium contains: glucos...
Embodiment 3
[0027] This example is used to illustrate the use of 50 m 3 Method for fermenting and producing citric acid in reactor using membrane tube aeration
[0028] First, the culture medium is sterilized, and the stirring motor is turned on at a speed of 100 r / min, so that the fermentation broth is evenly heated. When the temperature rises above 95 degrees, the stirring can be stopped. Carry out the sterilization of the air filter and the air pipeline again, and slightly open the filter and the drain valve to ensure the sterilization of the air pipeline. Finally, the outlet, sampling valve, steam valve and outlet valve are slightly opened to ensure that the pipeline is sterilized.
[0029] Fermentation culture and results: According to the inoculation amount of 10%, citrate-producing Aspergillus niger (purchased from CGMCC, the preservation number of Aspergillus niger is CGMCC5343) was inserted into a 35m 3 50m of fermentation medium 3 In an aerated reactor using membrane tubes (...
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