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Application and construction method of high-selenium induced insulin resistance animal model

A technology of insulin resistance and animal models, applied in the direction of effective components of sulfur/selenium/tellurium, etc., can solve the problems of narrow therapeutic window of selenium, negative effects of selenium on human body, etc., achieve reduction of phosphorylation level of protein kinase B, overcome research and Restrictions on the development of related drugs and the effect of high molding rate

Inactive Publication Date: 2013-07-24
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

However, due to the narrow therapeutic window of selenium, excessive levels of selenium may also cause negative effects on the human body

Method used

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  • Application and construction method of high-selenium induced insulin resistance animal model
  • Application and construction method of high-selenium induced insulin resistance animal model
  • Application and construction method of high-selenium induced insulin resistance animal model

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Embodiment Construction

[0027] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0028] The present invention is administered according to a dosage of 100 μg / kg-200 μg / kg every day to induce; high selenium induces glucose and lipid metabolism disorder, and then induces the occurrence of insulin resistance. The animal model of insulin resistance constructed by this method has characteristics similar to those of human high selenium insulin resistance.

[0029] The measurement results of the constructed animal model of insulin resistance showed that: the glucose tolerance and insulin tolerance of the animals in the model group were significantly different from those in the normal control group, the phosphorylation level of protein kinase B stimulated by insulin in the animals in the model group was significantly reduced, and the key to gluconeogenesis in the model group was ...

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Abstract

The invention discloses an application and construction method of a high-selenium induced insulin resistance animal model. The application and construction method comprises the following steps of: feeding 100mug / kg-200ug / kg of selenium to the stomach of an SD (Sprague Dawley) rat every day; and six weeks later, obtaining the insulin resistance animal model. According to the statistic analysis of glucose tolerance and insulin tolerance, insulin signal transduction and gluconeogenesis key enzyme test, the construction of the insulin resistance animal model by utilizing sodium selenite is feasible; and the insulin resistance animal model can be used as a high-selenium induced insulin resistance animal model and a pathogenesis and drug action mechanism research model of diabetes mellitus II. The insulin resistance rat model constructed by the application and the construction method disclosed by the invention has the advantages of simpleness in modeling method, high model establishing success rate, low cost, short period, good reproducibility and the like.

Description

technical field [0001] The invention belongs to the technical field of diabetes prevention and treatment, and relates to the application and construction method of an animal model of insulin resistance induced by high selenium. Background technique [0002] The construction of animal models is a very important technique in medical research. Constructing a disease model similar to human disease can overcome the constraints of long incubation period, many influencing factors, slow development and complex pathogenesis of human disease, better reflect the law of disease occurrence and development, and facilitate the study of different diseases. Diseases that are easy to study in humans themselves. Commonly used animal models mainly include spontaneous animal models, transgenic animal models and experimental animal models. Spontaneous animal models and transgenic animal models have high application value, but the high cost of modeling and high requirements for technical breedin...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04
Inventor 王欣海春旭
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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