Anthocyanin radish breeding method

Abstract

The invention discloses an anthocyanin radish breeding method. The method comprises the following steps: (1) selecting a female parent and a male parent to hybridize; (2) continuously utilizing a sterile plant in a filial generation as the female parent to continuously back cross with the original male parent; (3) after back crossing for 3-4 generations, finding a sensitive sterile plant with a micro amount of pollen in a flowering period to carry out bud stage selfing; (4) taking a male sterile plant as the female parent to test cross with the original male parent; and (5) selecting a system with stable sterility, anthocyanin content being more than 160mg / 100g fresh weight, thioglycodise content being less than 95mg / 100g fresh weight, excellent comprehensive characters and good combining capability as a dominant male sterile line, and obtaining a back cross male parent which is a maintainer line. The anthocyanin radish bred by adopting the method has the anthocyanin content being more than 167mg / 100g fresh weight and the thioglycodise content being less than 90mg / 100g fresh weight; and the anthocyanin radish has the advantages of difficult hollowness, difficult inner flowering, disease resistance, bolting resistance and stable performance, so that anthocyanin can be conveniently extracted and processed.

Description

technical field [0001] The invention relates to anthocyanin radish, in particular to a breeding method for preparing anthocyanin radish by using a dominant male sterile line, belonging to the technical field of plant breeding. Background technique [0002] Anthocyanin radish is low in price, abundant in sources and stable in pigmentation, and is extremely competitive in the market. In 2012, the international market price of anthocyanins from different sources reached 700-1500 US dollars / kg. Anthocyanins are mainly produced in South America, Europe, America, Asia and other places. South America mainly produces anthocyanins from purple corn, and Europe uses grapes, elderberries, blueberries, gooseberries, wild cherries and other fruits as raw materials. relatively expensive. In my country, radish, grapes and other raw materials are the main sources of anthocyanins, and radish anthocyanins have received great attention due to their excellent stability. At present, self-incom...

AI Technical Summary

Problems solved by technology

At present, self-incompatibility lines are mainly used to breed anthocyanin radish in production. Since self-incompatibility lines are used to prepare the first generation of anthocyanin radish hybrids, the cost of parental reproduction is high, and the hybridization rate of seeds is difficult to reach 100%.

In addition, the existing anthocyanin radish populations generally have mixed leaf color, leaf shape, and root shape, and degeneration phenomena such as early shoots and bran hearts, and the yield is generally not high, resulting in low anthocyanin content and high glucosinolate content.