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Method for preparing malachite green oxalate artificial antigen

A technology of artificial antigen and artificial hapten, which is applied in the direction of animal/human protein, material inspection product, albumin peptide, etc., can solve the problems of expensive equipment, time-consuming detection, and unreachability, and achieve advanced synthesis technology and strong specificity Effect

Inactive Publication Date: 2013-07-17
HANGZHOU HONGTAI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the detection of aniline green mainly relies on high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), simple and simple (MS), etc., but the equipment is expensive, the detection is time-consuming, and professional technicians are required. The operation cannot meet the fast and accurate requirements of modern detection

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  • Method for preparing malachite green oxalate artificial antigen
  • Method for preparing malachite green oxalate artificial antigen
  • Method for preparing malachite green oxalate artificial antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: a kind of preparation method of aniline green artificial antigen, carry out according to the following steps:

[0036] (1), preparation of artificial hapten --- the synthesis of hapten 4-MGC:

[0037] (a) Add N,N-dimethylaniline and 4-carboxybenzaldehyde with a mass molar ratio of 2:1 into a round bottom flask. The amount of N,N-dimethylaniline is 3g. The reaction was stirred for 6 hours to form a reaction solution; after the reaction was completed, the reaction solution was extracted with ether, dried, and a white solid product I was obtained after recrystallization;

[0038] (b), add 30ml of benzene and the catalytic amount of Amberlyst 15 resin to the product I and put them into a round-bottomed flask together, under the protection of nitrogen, reflux and stir overnight at a temperature of 130°C to form a solvent; The solvent was dried under reduced pressure and extracted with ether to obtain the product II;

[0039] (c), after the produc...

Embodiment 2

[0048] Embodiment 2: a kind of preparation method of aniline green artificial antigen, carry out according to the following steps:

[0049] (1), preparation of artificial hapten --- the synthesis of hapten 4-MGC:

[0050] (a) Add N,N-dimethylaniline and 4-carboxybenzaldehyde with a mass molar ratio of 2:1 into a round bottom flask. The amount of N,N-dimethylaniline is 3g. The reaction was stirred for 6 hours to form a reaction solution; after the reaction was completed, the reaction solution was extracted with ether, dried, and a white solid product I was obtained after recrystallization;

[0051] (b), add 30ml of benzene and the catalytic amount of Amberlyst 15 resin to the product I and put them into a round-bottomed flask together, under the protection of nitrogen, reflux and stir overnight at a temperature of 130°C to form a solvent; The solvent was dried under reduced pressure and extracted with ether to obtain the product II;

[0052] (c), after the produc...

Embodiment 3

[0061] Embodiment 3: a kind of preparation method of aniline green artificial antigen, carry out according to the following steps:

[0062] (1), preparation of artificial hapten --- the synthesis of hapten 4-MGC:

[0063] (a) Add N,N-dimethylaniline and 4-carboxybenzaldehyde with a mass molar ratio of 2:1 into a round bottom flask. The amount of N,N-dimethylaniline is 3g. The reaction was stirred for 6 hours to form a reaction solution; after the reaction was completed, the reaction solution was extracted with ether, dried, and a white solid product I was obtained after recrystallization;

[0064] (b), add 30ml of benzene and the catalytic amount of Amberlyst 15 resin to the product I and put them into a round-bottomed flask together, under the protection of nitrogen, reflux and stir overnight at a temperature of 130°C to form a solvent; The solvent was dried under reduced pressure and extracted with ether to obtain the product II;

[0065] (c), after the produc...

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Abstract

The invention relates to a malachite green oxalate artificial antigen, in particular to a method for preparing a malachite green oxalate artificial antigen. The method sequentially includes steps of synthesizing prepared artificial hapten and hapten 4-MGC (methyl glycol chitosan); and synthesizing a prepared malachite green oxalate artificial antigen, malachite green oxalate and BSA (bovine serum albumin). The method for preparing the malachite green oxalate artificial antigen has the advantages that a synthesizing process is advanced, the specificity is high, as shown by detection results obtained after the prepared malachite green oxalate artificial antigen is used for immune New Zealand white rabbits, the titer of immune sera of the malachite green oxalate artificial antigen is 1:72000, the malachite green oxalate artificial antigen is completely applicable to immunoassay, and a convenient, speedy and accurate channel for detecting malachite green oxalate is provided.

Description

[0001] technical field [0002] The invention relates to an aniline green artificial antigen, in particular to a preparation method of aniline green artificial antigen. Background technique [0003] Aniline green is also called malachite green in Chinese; Chinese green; alkaline malachite green; magenta green; For ethanol, methanol and amyl alcohol, the aqueous solution is blue-green, and the pH below 0.0 is yellow; the maximum absorption wavelength is 616.9nm. It is a toxic triphenylmethane chemical, which is not only a dye, but also a fungicide, and can cause cancer. Its structural formula is: [0004] [0005] The chemical functional group triphenylmethane in aniline green can cause cancer, and many countries have banned it, but fishermen still use it to prevent fungal infections in fish. It is also used by transporters as a disinfectant to prolong the survival time of fish in long-distance trafficking. Although the Ministry of Agriculture of China included mala...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765G01N33/53
Inventor 沈海英徐建明
Owner HANGZHOU HONGTAI BIOLOGICAL TECH
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