Odontobutisobscura temperature-control flowing-water hatching method

A technology of sand snakehead and running water, which is applied in the field of incubation of sand snakehead fertilized eggs in temperature-controlled running water, can solve problems that have not been seen before, improve the hatching rate of sand pond snakehead, achieve simple operation, shorten incubation time, and avoid the spread of mold and rot Effect

Inactive Publication Date: 2013-07-17
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Well-known reason, hatching rate is the precondition of frying rate of fry, that is to say, only have to improve hatching rate, the seeding rate of frying just has guarantee, yet in disclosed patent and non-patent literature, all do not see that How to improve the ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A) Obtaining fertilized eggs; the natural spawning method to obtain the fertilized eggs of Channa channa, specifically, use a small blade to directly peel off the egg mass from the attachment. The male and female broodstock of the snakehead are placed in the same breeding pond according to the ratio (female to male ratio is 2:1), and they are allowed to mate freely and lay eggs on the attachments, such as clam shells, tiles and stones placed in the breeding ponds. and so on, to obtain the fertilized eggs of Channa mongolica;

[0025] B) Incubation, take a small amount of fertilized eggs of sand pond snakehead and observe under a microscope to determine the developmental period or degree of development of the fertilized eggs. The developmental period of the fertilized eggs mentioned here refers to confirming that the fertilized eggs have been fertilized and completed the fertilized egg cells Splitting, the blastocyst stage has been completed and the embryo development is...

Embodiment 2

[0029] A) Obtaining fertilized eggs; the artificial insemination method is used to obtain the fertilized eggs of Channa mongolica. The artificial insemination method is to select brood fish whose male and female gonads mature synchronously. In a beaker of 5ml of normal saline (the 5ml of normal saline is for a pair of testes of a male broodstock), the capacity of the beaker is 100ml, and the testis is cut (shredded) with scissors and tweezers, and then the sperm Put it under a microscope to confirm the motility (mobility) of the sperm. After confirming that good sperm (motile sperm account for more than 70%) are obtained, extract the female sandfish broodstock with a full abdomen and gently press the abdomen of the female fish Squeeze out the cohesive eggs into the aforementioned beaker filled with sperm, and shake the beaker while squeezing to combine the sperm and eggs to obtain fertilized eggs, that is, artificially inseminated fertilized eggs, and pour the fertilized eggs i...

Embodiment 3

[0034] Only change the mass percent concentration of normal saline in step A) to 0.8%, and the amount of normal saline to 8ml (the amount for a pair of testes of a male broodstock); change the time of gently stirring with feathers to 3min; Change the density of the fertilized eggs in the hatching bucket in step B) to 20 grains / ml, change the incubation temperature when the fertilized eggs start to hatch to 20°C, and change the temperature at which the fertilized eggs develop to the gastrula stage after 3 days. The temperature was 28°C, the incubation time was changed to 15 days (15 days), and the hatching rate was 71%. All the other are the same as the description to embodiment 2.

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Abstract

The invention discloses an odontobutisobscura temperature-control flowing-water hatching method, and belongs to the technical field of fish-egg hatching. The method includes: fertilized eggs of the odontobutisobscura are obtained by means of an artificial fertilization method or a natural spawning method, the fertilized eggs of the odontobutisobscura obtained by means of the artificial fertilization method are unsticked, and the fertilized eggs of the odontobutisobscura obtained by means of the natural spawning method are fertilized eggmass which are delivered naturally by the odontobutisobscura and directly detached from an attachment by using a tool; a small amount of the fertilized eggs of the odontobutisobscura are extracted to be observed under a microscope to have growth periods of the fertilized eggs determined, a still water-changed method or a flowing water aeration method are adopted to hatch early embryo of archenteron, an up-flowing-water hatch method is adopted to hatch late embryo of the archenteron till the fertilized eggs detached from the attachment to hatch odontobutisobscura larva fishes; the odontobutisobscura larva fishes are collected prior to water operation in a seedling box made of silk cloth; and the larva fishes are introduced to a larva fish breeding pond after bladders appear on the larva fishes and summer flower seedings of the odontobutisobscura are obtained. By the temperature-control and the water-hatching method, the technical problem of easy mildew rot of the fertilized eggs of the odontobutis is solved, time for hatching is shortened, and hatching rate of the fertilized eggs is improved.

Description

technical field [0001] The invention belongs to the technical field of hatching fertilized eggs of fish, and in particular relates to a temperature-controlled running water hatching method for fertilized eggs of sandfish. Background technique [0002] Sandfish (Latin: Odontobutis obscurus ), belongs to Perciformes, Pond Channaidae, Sand Pond Channa. Common names: Black Grass Bun, Grass Po Fish, Tubu Fish, Pond Channa, Tucai Fish, Tuhanba, etc. In my country, it is mainly distributed in the middle and lower reaches of the Yangtze River and its tributaries, the Qiantang River system and the Minjiang River system and other vast areas. Although the individual of sand pond snakehead is small, it has the characteristics of high meat content, few spines, tender and delicious meat and rich nutrition. Every 100 grams of channa meat contains 18.39g of protein, 0.98g of fat, 0.42g of calcium, phosphorus, iron and vitamins. The essential amino acids and umami amino acids contained i...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐建荣韩晓磊杨俊杰曹昆齐刚
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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