Application of mesenchymal stem cells in preparation of psoriasis treatment medicines, and extraction method of mesenchymal stem cells
A technology for stromal stem cells and psoriasis, which is applied to the application field of mesenchymal stem cells and their extraction in the preparation of medicines for psoriasis, and can solve the problems of undiscovered immune regulation function of skin mesenchymal stem cells and the like.
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[0033] 1. Isolation of mouse dermal mesenchymal stem cells:
[0034] Take out the newborn mice about 3 days old, kill them by necking, disinfect with alcohol, cut the back skin and put it in 0.25% dispase enzyme, digest it overnight at 4°C, wash it with PBS solution for 3 times, peel off the epidermis, and place the dermis in 0.2% dispase After the digestion, add DNase I to digest for 10 to 15 minutes, wash it with PBS, blow it into a single cell suspension with a dropper, filter to remove large pieces of tissue and debris, and inoculate in a petri dish , the culture condition is DMEM (low glucose) + 10% fetal bovine serum, 37°C, 5% CO2. After culturing for 6 hours, discard the suspended cells gently, and continue culturing the adherent cells. After most of the bottom of the bottle is covered, trypsinize and routinely pass. Cells passed down to the third passage were taken for research.
[0035]
[0036] 2. Identification of mouse dermal mesenchymal stem cells:
[0037] De...
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