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RPRP (Rice Photorespiration Related Protein)-1 capable of increasing photosynthetic efficiency of plants as well as coding gene and application of RPRP-1

A technology encoding gene and photosynthetic efficiency, applied in the field of protein and its encoding gene and application, to achieve the effect of improving photosynthetic efficiency

Inactive Publication Date: 2013-06-26
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most critical technical bottleneck in this method is the screening and functional discovery of genes related to photosynthesis and photorespiration.

Method used

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  • RPRP (Rice Photorespiration Related Protein)-1 capable of increasing photosynthetic efficiency of plants as well as coding gene and application of RPRP-1
  • RPRP (Rice Photorespiration Related Protein)-1 capable of increasing photosynthetic efficiency of plants as well as coding gene and application of RPRP-1
  • RPRP (Rice Photorespiration Related Protein)-1 capable of increasing photosynthetic efficiency of plants as well as coding gene and application of RPRP-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1, the acquisition of rice photorespiration-related gene RPRP-1

[0052] 1. Screening of photosynthesis-related mutants in rice

[0053] The T2 generation seeds in the enhancer T-DNA insertion mutant library are screened, and the specific operation is as follows: 20-25 seeds are taken for each independent line, sterilized with 1% concentration (V / V) sodium hypochlorite solution, and shaken for 10 minutes , and then rinse the seeds with tap water for 10 minutes, soak the seeds to accelerate germination, and sow them in the soil (nutrient soil: frog stone = 3:1), plant in the greenhouse (photoperiod 16h / dark cultivation 8h), and observe for 15-30 days Seedling leaf color changes. The mutant lines were counted, and the specific phenotypes involved leaf albinism, yellowing, chlorosis, and variegated leaves. ( figure 1 )

[0054] 2. Screening and obtaining of mutants co-segregated with rice T-DNA insertion

[0055] Part of the leaves of the mutant that had grow...

Embodiment 2

[0081] Example 2, functional verification of RPRP-1 of the present invention and its coding gene

[0082] 1. Complementary Verification of RPRP-1

[0083] 1) Take part of the leaves of wild-type rice Nipponbare grown for 2 weeks, extract total RNA, reverse transcribe it into eDNA, use this eDNA as a template, and use SHMTr and SHMTf in Example 1 as primers to amplify the complete gene coding region , into the pEASY-T1simple vector for sequencing.

[0084] 2) the rice Actin promoter (its nucleotide sequence can be as shown in SEQ ID No. 3 in the sequence listing) sequence is inserted between the multiple cloning site Kpn I of the plasmid pCambia2300 to obtain the intermediate vector pCambia2300A;

[0085] 3) The nucleotide sequence containing the RPRP-1 gene sequenced correctly in step 1) was digested from the pEASY-T1 simple vector and connected between the multiple cloning sites SmaI and SalI of the pCambia2300A plant binary expression vector to construct To form a compleme...

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Abstract

The invention discloses an RPRP (Rice Photorespiration Related Protein)-1 capable of increasing the photosynthetic efficiency of plants as well as a coding gene and application of the RPRP-1. The RPRP-1 is protein with one of the following amino acid residue sequences: (1) an amino acid residue sequence as shown in SEQ ID No.2 in a sequence table; and (2) an amino acid residue sequence obtained through substituting and / or deleting and / or adding one or more amino acid residues of the amino acid residue sequence shown as SEQ ID No.2 in the sequence table, wherein the protein is relevant to the photosynthetic efficiency of plants and derived from (1). The RPRP-1 provided by the invention can be used for increasing the net photosynthetic rate, the stomatal conductance and the thousand seed weight of the plants. The coding gene of the RPRP-1 provided by the invention can have a favorable application prospect in breeding monocots such as rice, wheat, corn, pasture and wheatgrass at high photosynthetic efficiency.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a protein capable of improving photosynthetic efficiency of plants, its coding gene and its application. Background technique [0002] Food security is an overall major strategic issue related to my country's national economic development and social stability. With the development of industrialization, urbanization and population increase, my country's food demand will show a rigid growth, while the constraints on food production such as the reduction of cultivated land, water shortage, and climate change are becoming increasingly prominent, and my country's food supply and demand will be in a "tight balance" for a long time State, ensuring food security is facing severe challenges. Plant type breeding and heterosis utilization of crops have made great contributions to the increase of grain crop yield and the rapid development of national economy in our country. Under the current back...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12N15/63C12N5/10C12N1/21C12N15/84A01H5/00
Inventor 吴金霞张治国孙学辉张芊路铁刚
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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