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2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium se08, and screening method and application thereof

A technology of dimethyltetrachlorine herbicide and screening method, which is applied in the field of environmental pollution bioremediation and can solve the problems of few reports on dimethyltetrachlorine microbial degradation

Inactive Publication Date: 2013-06-26
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scholars at home and abroad have done a lot of research on the microbial degradation of herbicides, but there are few reports on the microbial degradation of dimethyltetrachloride

Method used

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  • 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium se08, and screening method and application thereof
  • 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium se08, and screening method and application thereof
  • 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium se08, and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation, Purification and Identification of Dimethyltetrachloride Herbicide Degrading Bacteria SE08

[0028] 1. Separation and purification of dimethyltetrachloride herbicide-degrading bacteria SE08

[0029] Take 10.0g of the polluted soil sample into the Erlenmeyer flask, add 100mL of inorganic salt culture solution containing 50mg / L dimethyltetrachloride, shake and culture at 30°C and 200rpm for 5 days; take out 10mL of shaking solution and add it to 90mL Inorganic salt culture solution of tetrachloride, continue shaking culture at 30°C and 200rpm for 5 days, and so on, increase the concentration of dimethyltetrachloride to 250, 500, 750, 1000 mg / L respectively, take out 10mL of culture solution and add In 90mL of dimethyltetrachloride inorganic salt culture solution containing gradient increasing concentration, continue shaking culture at 30°C and 200rpm for 5 days; take the culture in 1000mg / L dimethyltetrachloride inorganic salt culture solution and add agar (20g...

Embodiment 2

[0039] Growth and degradation characteristics test of degrading bacteria

[0040] Use high-performance liquid chromatography (HPLC) to analyze and determine the residual amount of dimethyltetrachloride in the inorganic salt culture solution, and the amount of bacterial growth can be determined by measuring the OD of the sample. 600nm to make sure.

[0041] (1) Dimethyl tetrachloride degradation kinetics and bacterial strain SE08 growth curve: 3mL bacterial suspension (OD 600nm ≈0.3) into 100mL inorganic salt culture solution containing 500mg / L dimethyltetrachloride, pH 6.0, cultured at 30°C for 72h, the results are shown in Figure 4 , cultivated for 36h, OD 600nm The maximum value is 0.64. In the later stage, due to the degradation of dimethyltetrachloride, it cannot provide enough carbon source and energy, resulting in the death of the bacteria, OD 600nm decreased, the OD of 72h 600nm is 0.64. With the increase of the number of bacteria, the degradation rate of dimethyl...

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Abstract

The invention belongs to the field of biological repair of environmental pollution, and particularly relates to a 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08, and a screening method and application thereof. The 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08 is Enterobacter sp. of which the collection number is CGMCC No.7016 and the collection date is 2012.12.18; and the 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08 is collected at China General Microbiological Culture Collection Center. The invention also discloses a screening method and application of the 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08. The 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08 is obtained by primarily screening in 2-methyl-4-chlorophenoxyacetic acid heavily-polluted soil in the tobacco growing area in Chenzhou City of Hunan Province, rescreening and separation purification; and the degrading bacterium can effectively degrade 2-methyl-4-chlorophenoxyacetic acid residues in the environment, and is applicable to biological repair in 2-methyl-4-chlorophenoxyacetic acid polluted soil and water.

Description

technical field [0001] The invention belongs to the field of environmental pollution bioremediation, in particular to a dimethyltetrachloride herbicide-degrading bacterium SE08 and a screening method and application thereof. Background technique [0002] Since 1956, China has used phenoxycarboxylic acid herbicides on a large scale in rice fields and tobacco fields. Among them, dimethyltetrachloride herbicides are widely used because of their remarkable weeding effect and low price. Dimethyl tetrachloride herbicide is a hormone-type systemic conduction selective phenoxycarboxylic acid herbicide, which is easily absorbed by the roots and leaves of plants. It has strong physiological activity on plants. It promotes growth at low concentrations and inhibits it at high concentrations. grow. However, dimethyltetrachloride is easy to enter the aquatic system, and its toxicity to aquatic organisms cannot be ignored. Dimethyltetrachloride is easy to transfer on the soil surface and...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02B09C1/10C02F3/34C12R1/01C02F101/36
Inventor 谭琳曾维爱郑雄志周志成李宏光胡秋龙谭济才江紫薇
Owner HUNAN AGRICULTURAL UNIV
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