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BMP-2 (Bone Morphogenetic Protein-2) sustained release microsphere and preparation method thereof

A technology of BMP-2 and sustained-release microspheres, which is applied in pharmaceutical formulations, medical preparations without active ingredients, and medical preparations containing active ingredients, etc. It can solve the problem of uneven BMP-2 microspheres and low encapsulation rate. , poor dispersibility and other problems, to achieve the effect of good biocompatibility and protein activity, rapid response, and maintenance of activity

Inactive Publication Date: 2013-06-26
GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to regulate the expression of related genes in the nucleus by BMP-2, induce the differentiation of cells to promote the formation of cartilage and bone, and then provide a BMP-2 slow-release microsphere and its preparation method, which solves the problem of traditional emulsification method preparation. Problems of uneven loading of BMP-2 microspheres, poor dispersion and low encapsulation efficiency

Method used

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  • BMP-2 (Bone Morphogenetic Protein-2) sustained release microsphere and preparation method thereof
  • BMP-2 (Bone Morphogenetic Protein-2) sustained release microsphere and preparation method thereof

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Embodiment 1

[0028] A preparation method of BMP-2 slow-release microspheres, comprising the following steps:

[0029] 1) Dissolve chitosan with a molecular weight of 90-150KD and a deacetylation degree of 85% in 0.175% (volume) acetic acid aqueous solution to prepare a chitosan solution with a concentration of 1mg / ml;

[0030] 2) dissolving dextran sulfate sodium with a molecular weight of 500KD in an aqueous solution to prepare a dextran sulfate sodium aqueous solution with a concentration of 1 mg / ml;

[0031] 3) In the reaction vessel, according to BMP-2, dextran sulfate sodium weight ratio is 2: 10, BMP-2 is dissolved in dextran sulfate sodium aqueous solution, under magnetic stirring condition, chitosan solution is mixed with Add chitosan and dextran sulfate sodium at a weight ratio of 6:10 and drop them into the above solution, then add ZnSO 4 (the dosage accounts for 5% of the weight of the reaction system), microspheres are formed in the reaction solution, the stirring is stopped, ...

Embodiment 2

[0034] A preparation method of BMP-2 slow-release microspheres, comprising the following steps:

[0035] 1) Dissolve chitosan with a molecular weight of 90-150KD and a deacetylation degree of 85% in 0.175% (volume) acetic acid aqueous solution to prepare a chitosan solution with a concentration of 1mg / ml;

[0036] 2) dissolving dextran sulfate sodium with a molecular weight of 500KD in an aqueous solution to prepare a dextran sulfate sodium aqueous solution with a concentration of 1 mg / ml;

[0037] 3) In the reaction vessel, according to BMP-2, dextran sulfate sodium weight ratio is 2: 10, BMP-2 is dissolved in dextran sulfate sodium aqueous solution, under magnetic stirring condition, chitosan solution is mixed with Chitosan, dextran sulfate sodium weight ratio is 3:10 drop into the above solution, then add ZnSO 4 (the dosage accounts for 5% of the weight of the reaction system), microspheres are formed in the reaction solution, the stirring is stopped, the microspheres are ...

Embodiment 3

[0040] A preparation method of BMP-2 slow-release microspheres, comprising the following steps:

[0041] 1) Dissolve chitosan with a molecular weight of 90-150KD and a deacetylation degree of 85% in 0.175% (volume) acetic acid aqueous solution to prepare a chitosan solution with a concentration of 1mg / ml;

[0042]2) dissolving dextran sulfate sodium with a molecular weight of 500KD in an aqueous solution to prepare a dextran sulfate sodium aqueous solution with a concentration of 1 mg / ml;

[0043] 3) In the reaction vessel, according to BMP-2, dextran sulfate sodium weight ratio is 2: 10, BMP-2 is dissolved in dextran sulfate sodium aqueous solution, under magnetic stirring condition, chitosan solution is mixed with Add chitosan and dextran sulfate sodium at a weight ratio of 6:10 and drop them into the above solution, then add ZnSO 4 (the dosage accounts for 10% of the weight of the reaction system), microspheres are formed in the reaction solution, the stirring is stopped, ...

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Abstract

The invention discloses a BMP-2 (Bone Morphogenetic Protein-2) sustained release microsphere and a preparation method thereof. The preparation method of the BMP-2 sustained release microsphere comprises the following steps of: dissolving chitosan in an aqueous solvent so as to obtain a chitosan solution; dissolving BMP-2 into a dextran sulfate sodium aqueous solution, dropwise adding the chitosan solution into the dextran sulfate sodium aqueous solution under the condition of stirring, then adding a metal salt so as to form the microsphere in a reaction solution, washing and freeze-drying so as to obtain the BMP-2 sustained release microsphere. The matrix component of the microsphere prepared by using the method comprises chitosan and dextran sulfate sodium, the wrapped medicine is BMP-2, the average particle size of the microsphere is 200-900nm, the encapsulation efficiency can be 75-90%, and the BMP-2 is long in protein activity duration and sustained release period. The BMP-2 sustained release microsphere is simple in preparation process, low in production cost, easy to carry out, and mild in reaction condition, and does not need the addition of reagents which can influence the protein activity of BMP-2; and the e microsphere is round and complete in appearance, good in polydispersity, non-sticky after being freeze-dried and soluble in water.

Description

technical field [0001] The invention relates to a BMP-2 slow-release microsphere and a preparation method thereof. Background technique [0002] Bone tumors and severe bone trauma may cause bone defects. Bone defects have always been a difficult problem for orthopedic surgeons. With the deepening of bone defect repair research, various bone repair materials emerge in an endless stream, mainly including autologous bone, allogeneic bone and artificial bone. Autologous bone has few sources and absorbs quickly, while allogeneic bone and xenograft bone have immune rejection, and there is a risk of potential infectious diseases. Artificial bone has become a hot spot in the research of bone repair materials because of its wide range of materials, easy processing, good biocompatibility, low price, disinfection and convenience for clinical operation. [0003] Molecular biological studies have shown that fracture healing is a complex process regulated by a variety of cell growth fac...

Claims

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Application Information

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IPC IPC(8): A61K38/19A61K9/16A61K47/36A61P19/08A61L27/54
Inventor 夏远军尹庆水夏虹章莹钱翠芳
Owner GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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