Liquid-phase chip kit for acute coronary syndrome and preparation method for same

Abstract

The invention relates to a liquid-phase chip kit for acute coronary syndrome and a preparation method for the same. A liquid-phase chip mainly comprises microspheres coated with oxidized low density lipoprotein (ox-LDL), soluble CD40 ligand (sCD40L), matrix metallopeptidase 9 (MMP-9), tissue factor (TF), omentin and endogenous secretory receptor of advanced glycation end products (esRAGE) capture antibody respectively, detection antibodies labelled by biotin, and streptavidin phycoerythrin. The liquid-phase chip provided by the invention has the advantages of being high in detection efficiency, low in the quantity of the needed samples, strong in specificity, high in sensitivity, rapid and accurate in detection, and the like. Simultaneously, the chip reflects a protective factor level and an injury factor level in an organism, so that the risk stratification and prognosis conditions of a patient can be much comprehensively assessed. Simultaneously, the preparation method disclosed by the invention is simple and practicable, and good in stability, various process parameters such as the quantities of the microspheres and the antibodies, and the reaction process in the technical scheme of the preparation method are obtained on the basis of lots of experiments, and are the optimal parameter values of the preparation process.

Description

Technical field: [0001] The invention relates to the technical field of in vitro kits, in particular to a liquid phase chip kit for acute coronary syndrome and its preparation method. Background technique: [0002] Atherosclerosis (AS) vascular disease has become the "number one killer" that threatens the health of Chinese people. With the development of my country's economy, the incidence rate is increasing year by year. The annual medical expenses for such diseases in my country are as high as 100 billion Strengthening the prevention and treatment of AS vascular diseases and their complications has become the main task of the prevention and treatment of major diseases in our country. [0003] Acute coronary syndrome (ACS) caused by AS vascular disease is the most common and serious clinical manifestation, and the rupture of vulnerable coronary plaque followed by thrombosis is the main pathological basis of its occurrence. At present, coronary angiography, which is the "gol...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide an improved liquid-phase chip kit for acute coronary syndrome and its preparation method, which can overcome the diagnostic kits in the prior art and focus on improving the detection of ACS by means of peripheral serum markers. In terms of diagnostic sensitivity and specificity, there is still a gap in the relevant kits for improving the risk stratification and prognosis of patients. At the same time, the existing kits have some shortcomings such as complex preparation methods, poor stability, and poor application effects.

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