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Production process of feeding lactobacillus microcapsule and microcapsule, application and premix thereof

A lactic acid bacteria microcapsule and production process technology, applied in feed, application, animal feed and other directions, can solve the problems of uneven dispersion of lactic acid bacteria, high mortality of lactic acid bacteria, low yield of lactic acid bacteria microcapsules, etc. The effect of convenient preparation process and long storage period

Active Publication Date: 2013-06-19
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of the prior art that lactic acid bacteria with high live bacteria content cannot be produced at normal temperature, the mortality rate of lactic acid bacteria is high during the preparation process, the dispersion of lactic acid bacteria is uneven, and the yield of lactic acid bacteria microcapsules is low, and to provide a Production process for preparing microcapsules of lactic acid bacteria for feeding

Method used

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  • Production process of feeding lactobacillus microcapsule and microcapsule, application and premix thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. The selected strain is Lactobacillus acidophilus CGMCC No.5093.

[0048] 2. Preparation of Lactobacillus acidophilus fermentation broth

[0049] (1) Lactobacillus acidophilus strain activation;

[0050] Incubate the freeze-dried Lactobacillus acidophilus in the ampoule with 2mL sterile normal saline and incubate at a constant temperature of 37°C until the liquid becomes turbid, then inoculate it in MRS liquid medium at a rate of 5% in an aseptic manner, and incubate at a constant temperature of 37°C 24h, repeated activation 2-3 times.

[0051] (2) preparation of Lactobacillus acidophilus fermentation broth;

[0052] The seed medium of Lactobacillus acidophilus is: glucose 10g, peptone 5g, beef extract 4g, sodium chloride 3g, calcium carbonate 1g, add water to 1000mL, pH value is 7.0, cultivate for 24 hours and prepare for replanting.

[0053] The fermentation medium of Lactobacillus acidophilus is: glucose 20g, peptone 5g, beef extract 4g, sodium chloride 3g, calc...

Embodiment 2

[0073] 1. The strain selected is Bifidobacterium BB12.

[0074] 2. Preparation of Bifidobacterium BB12 fermentation broth

[0075] (1) Bifidobacterium BB12 strain activation;

[0076] Incubate the freeze-dried Bifidobacterium BB12 strain in the ampoule tube with 2mL sterile normal saline at a constant temperature of 37°C until the liquid becomes cloudy, then inoculate it in MRS liquid medium at a rate of 5% in an aseptic manner, and incubate at a constant temperature of 37°C 24h, repeated activation 2-3 times.

[0077] (2) preparation of bifidobacterium BB12 fermented liquid;

[0078] The seed medium of Bifidobacterium BB12 is: MRS liquid medium, ready to replant after 24 hours of cultivation.

[0079] The fermentation medium of bifidobacterium BB12 is: glucose 20g, soybean peptone 5g, tryptone 15g, corn dregs 10g, citric acid 8g, soybean peptide 8g, mixed salt solution (anhydrous CaCl 2 0.2g, KH 2 PO 4 1.0g, MgSO 4 .7H 2 O0.48g, NaHCO 3 10.0g, NaCl 2.0g, distilled wa...

Embodiment 3

[0092] 1. The strain selected is Lactobacillus plantarum.

[0093] 2. Preparation of Lactobacillus plantarum fermentation broth

[0094] (1) Activation of Lactobacillus plantarum strains;

[0095] Incubate the freeze-dried Lactobacillus plantarum strains in the ampoule with 2mL sterile normal saline at a constant temperature of 37°C until the liquid becomes turbid, then inoculate it in MRS liquid medium at a rate of 5% in an aseptic manner, and incubate at a constant temperature of 37°C for 24 hours , repeated activation 2-3 times.

[0096] (2) preparation of Lactobacillus plantarum fermentation liquid;

[0097] The seed medium of Lactobacillus plantarum is: MRS liquid medium, ready to replant after 12 hours of cultivation.

[0098] The fermentation medium of Lactobacillus plantarum is (1L): 15g soybean peptone, 5g yeast extract, 5g D-fructose, 15g glucose, 15g sodium citrate, 2g dipotassium hydrogen phosphate, 5g sodium acetate, MgSO 4 .7H 2 O is 0.58g, MnSO4 0.25g Tween...

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Abstract

The present invention relates to the technical field of lactobacillus coating, specifically relates to a production process of feeding lactobacillus microcapsules and microcapsules, application and a premix thereof. Trehalose and lactose which can rapidly form vitrification protection state at a low temperature are used, sodium glutamate, manganese sulfate, glycerin, and polyvinyl pyrrolidone are used as main components for a wall material, special ultrasonic wave spray drying equipment is used, an ultrasonic spray nozzle is used for atomization, vacuum and a protective gas such as nitrogen are used for drying at room temperature, thus the effects of low temperature of low temperature freeze-drying and high temperature of high temperature spray drying on lactobacillus activity are prevented, and survival rate of the bacteria can reach more than 90%. The process of the invention achieves that lactobacillus is dried at room temperature, the preparation process is easy to control, the obtained product has a long storage period, and the storage time of the product can be significantly extended.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria coating, in particular to a production process of feeding lactic acid bacteria microcapsules, the microcapsules, their application and a premix. Background technique [0002] Antibiotics have always played an important role in poultry farming, but their negative effects have not been resolved, such as the spread of drug resistance, drug residues, and ecological damage. With the enhancement of people's awareness of food safety, antibiotics are increasingly questioned and resisted by people, and now many countries have banned the use of antibiotics in poultry farming. As a green feed additive, the probiotics of lactic acid bacteria for feeding is a good substitute for antibiotics. Lactic acid bacteria are resident beneficial bacteria in animals, which can antagonize pathogenic microorganisms, regulate the balance of animal digestive tract microflora; activate the immune system and enha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23N17/00
Inventor 王安如付维来杜建涛
Owner BEIJING DABEINONG TECH GRP CO LTD
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