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Method for immobilizing effective microorganisms (EM) during solid fermentation

A solid fermentation, EM bacteria technology, applied in chemical instruments and methods, fixed on/in organic carriers, water/sludge/sewage treatment, etc. Advantageous species and other issues, to achieve the effect of low price, long action time and good effect

Inactive Publication Date: 2013-06-12
GUANGXI INST OF FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

EM bacteria products are generally water and powder, because the bacteria of water and powder bacteria are easy to become free in water, it is difficult to form a dominant population, and the survival time of bacteria is short, thus greatly reducing its effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] optimal embodiment. Carry out the method for immobilizing EM bacteria in solid state fermentation, operation steps are as follows:

[0035] 1. Select fresh and clean Chinese fir sawdust, after drying at a low temperature below 60°C, use a 500-type wood flour machine with a sieve produced by Gongyi Zhangsheng Machinery Co., Ltd., and use a sieve with a mesh size of 0.425mm to crush twice , so that the sawdust becomes flocculent fiber wood powder.

[0036] 2. The weight percentage formula of the preparation culture agent is: sugarcane molasses 25%, humic acid 5%, NH 4 CI10%, KH 2 PO 4 2%, compound enzyme 0.7%, trace element 0.3%, tap water 57%. When preparing, first put in water, then slowly add sugarcane molasses, stir evenly, and then add other raw materials one by one, the prepared culture agent has a certain viscosity. Inject solid EM strains with a viable count of 3 billion / g, and the inoculum weight percentage is 10%. First, tap water with 4.05 times the weight...

Embodiment 2

[0040] Carry out the method for immobilizing EM bacteria in solid state fermentation, operation steps are as follows:

[0041] 1. Select fresh and clean poplar wood chips, after drying, use the 500 model wood flour machine with sieve produced by Gongyi Zhangsheng Machinery Co., Ltd., and use a sieve with a mesh size of 0.425mm to crush twice to make the sawdust Turn into flocculent fiber wood flour.

[0042] 2. The weight percentage formula of the preparation culture agent is: sugarcane molasses 20%, humic acid 4%, NH 4 CI8%, KH 2 PO 4 1%, compound enzyme 0.7%, trace element 0.3%, tap water 66%. When preparing, first put in water, then slowly add sugarcane molasses, stir evenly, and then add other raw materials one by one, the prepared culture agent has a certain viscosity. Inject solid EM strains with a viable count of 5 billion / g, and the inoculum weight percentage is 10%. First, tap water with 5.62 times the weight of the strains is added, and then the strains are put i...

Embodiment 3

[0046] Carry out the method for immobilizing EM bacteria in solid state fermentation, operation steps are as follows:

[0047] 1. Select fresh and clean willow sawdust, after drying at a low temperature below 60°C, use a 500-type wood flour machine with a sieve produced by Gongyi Zhangsheng Machinery Co., Ltd., and use a sieve with a mesh size of 0.425mm to crush twice , so that sawdust becomes flocculent fiber wood powder.

[0048] 2. The weight percentage formula of the preparation culture agent is: sugarcane molasses 30%, humic acid 6%, NH 4 CI12%, KH 2 PO 4 3%, compound enzyme 0.7%, trace element 0.3%, tap water 38%. When preparing, first put in water, then slowly add sugarcane molasses, stir evenly, and then add other raw materials one by one, the prepared culture agent has a certain viscosity. Insert the liquid EM bacterial classification that the number of viable bacteria is 1 billion / g, and the inoculum weight percentage is 10%, because it is a liquid bacterial cla...

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PUM

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Abstract

The invention discloses a method for immobilizing effective microorganisms (EM) during solid fermentation. The method is used for preparing immobilized EM through four steps including (1) preparation of a carrier, (2) preparation of fermented materials, (3) preparation of EM microbial inoculum through fermentation, and (4) preparation of immobilized solid EM microbial inoculum. Compared with the prior art, the method has the advantages that 1 operation time is shortened, work efficiency is improved, production cost is saved, effects are better than those of the mode that single adsorption immobilization is used in the prior art, and a problem that adsorption immobilization bacteria are easy to drop in the prior art is solved; and 2 EM preparation prepared by combining the adsorption of the carrier and bonding of a culture medium enables the bacteria to be tightly fixed on carrier fibers, and partial population superiority can be formed in a water body, so that action time of water quality is long, and effects are obvious.

Description

technical field [0001] The invention relates to solid fermentation of bacteria and a physical adsorption method in immobilized microorganism technology. Specifically, it is a method for immobilizing EM bacteria in solid fermentation. Background technique [0002] Immobilized microorganism technology is a modern bioengineering technology that uses chemical or physical means and methods to limit or locate free microorganisms in a specific space, retain their inherent catalytic activity, and can be used repeatedly and continuously. Because immobilized microorganism technology can greatly improve the purification effect of bacteria on water quality, it is widely used in sewage treatment, landscape water purification and aquaculture water quality adjustment. [0003] At present, there are many preparation methods for immobilized microorganisms, which are generally divided into two categories: physical immobilization methods (mainly embedding methods, adsorption methods and envel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C02F3/34
Inventor 周宇朱佳杰甘西唐瞻杨谭芸
Owner GUANGXI INST OF FISHERIES
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