Method for canna step-tip tissue culture

Abstract

The invention relates to a method for canna step-tip tissue culture, comprising the steps of culture medium preparation, selection and disinfection of an explant, stem-tip peeling, step-tip culture, adventitious bud induction and proliferation, adventitious bud root induction and tissue culture seedling acclimation and transplanting. According to the method, the basic culture medium adopts the MS (Murashige and Skoog) culture medium containing sucrose of 25-30 g / L and agar powders of 8-9 g / L, and the culture medium has the pH value of 5.7-5.8; the step-tip culture adopts an MS culture medium containing 6-BA (6-Benzylaminopurine) of 0.1-0.5 mg / L, NAA (1-naphthlcetic acid) of 0.05-0.02 mg / L and L-proline of 50-200 mg / L; the adventitious bud induction and proliferation adopts an MS culture medium containing 6-BA (6-Benzylaminopurine) of 2.0-6.0 mg / L and CaCl of 250-800 mg / L; and the root culture medium is an MS culture medium containing IBA (indolebutyric acid) of 0-0.5 mg / L or a 1 / 2 MS culture medium containing IBA (indolebutyric acid) of 0-0.5 mg / L. The method has the advantages that the built canna tissue culture rapid propagation technological system has fast seedling propagation speed, the seedlings are strong and uniform and the transplanting survival rate is high; and the method is suitable for seedling detoxification and scale production of canna.

Description

technical field [0001] The invention relates to plant tissue culture technology and plant shoot tip culture technology, mainly a method for canna shoot tip tissue culture. Background technique [0002] Canna (Canna indica), also known as Lanjiao and Tanhua, is a plant of the family Cannaceae and the genus Canna. It is a perennial bulbous herb flower native to tropical America and Africa. The plant height of canna can reach 100-180cm. It grows in clusters, with large leaves and rich colors, large flowers, bright colors, various colors, and long flowering period. It is a very good landscaping and landscaping plant. Canna leaves can absorb harmful substances such as sulfur dioxide and hydrogen chloride. Although the leaves are vulnerable, they can regenerate new leaves and quickly resume growth. Therefore, they can be used as indicators for monitoring harmful gas pollution, purifying the air and protecting the environment. In addition, canna can also be used as medicine to tre...

AI Technical Summary

Problems solved by technology

[0004] Plant shoot tip culture is considered to be the most effective means of obtaining plant detoxification materials at present. At present, there are very few reports on the rapid propagation of different varieties of canna at home and abroad, and the value-added efficiency is low, the highest is only 1.67. As for the research on canna shoot tip culture, there is no report Related reports