Tissue culture method for ornithogalum caudatum

Abstract

The invention relates to a tissue culture method for ornithogalum caudatum. The method comprises the following steps of: preparing culture media, selecting and sterilizing explants, primarily cultivating, inducing adventitious buds, growing of the adventitious buds, performing root induction on the adventitious buds, and domesticating and transplanting tissue culture seedlings. The basic culture medium selects an MS culture medium; the dosage of saccharose is 20-40 g / L; a coagulator is agar powder, and the dosage of the coagulator is 8-9 g / L; the pH of the culture medium is adjusted to 5.7-5.8 before being sub-charged; the initial culture medium comprises 0.1-0.5 mg / L of MS+6-BA and 0.05-0.2 mg / L of NAA; the adventitious bud induction culture medium comprises 2.0-4.0 mg / L of MS+6-BA and 0.05-0.2 mg / L of NAA; and the adventitious bud increment culture medium comprises 0.5-3.0 mg / L of MS+6-BA and 0.05-0.2 mg / L of NAA; and the rooting medium comprises 0-0.5mg / L of MS+IBA, or 0-0.5mg / L of 1 / 2MS+IBA. The method has the advantages that a built tissue culture and rapid propagation technique system for ornithogalum caudatum can reach a growth coefficient of 3-5, a rooting rate of 100% and a transplanting survival rate of 100%, and the tissue culture seedlings are healthy and even. Therefore, the method is suitable for detoxification and large-scale production of excellent ornithogalum caudatum seedlings.

Description

technical field [0001] The invention relates to plant tissue culture technology and plant shoot tip culture technology, mainly a squill tissue culture method. Background technique [0002] Sea onion (Ornithogalum caudatum), also known as Dieffenbachia tiger's eye, commonly known as glass ball flower, gourd orchid, etc., is a plant of the Liliaceae, Dieffenbachia tiger's eye, perennial bulbous herb flower, native to southern Africa, likes sunshine, avoids direct sunlight in summer, and also It is semi-shady and cold-resistant, and has a good humid environment. After heavy frost in winter, the foliage remains green. The plant height of squill is about 30-40cm when it is not in bloom, and the flower stem can reach 50-60cm in height when it blooms. It usually blooms from mid-April to early May every year. The flowers are cone-shaped inflorescences, and the flowers are white, orange and double. It is simple and has a long flowering period. It is an excellent material for arrangi...

AI Technical Summary

Problems solved by technology

[0003] At present, the propagation of squills is mainly carried out by seeds and bulbs, but the bulbs are usually used as bulbs for propagation in August to September every year, and the seedlings need to be cultivated for 3 to 4 years before they can bloom. Production is severely limited by seasons and years, and the number of annual reproduction is limited, so the promotion of squill is not used

At the same time, since squill bulbs are kept underground for a long time, they are easily infected by various viruses. If the bulbs are used for asexual reproduction, once the bulbs are infected with viruses, it will be difficult to eradicate them with chemicals, which will seriously reduce the ornamental value of squills.

[0004] Plant tissue culture technology is considered to be the most efficient means of rapid propagation of plant seedlings. At present, there are no literature reports on the rapid propagation of squills by plant tissue culture technology at home and abroad.