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Preparation method for fish eye socket active grease

A technology of active oil and fish eye socket, which is applied in the direction of fat oil/fat production and fat generation, can solve the problem of high oil content, achieve high recovery rate, advanced technology, and improve oil yield

Active Publication Date: 2013-06-05
DALIAN POLYTECHNIC UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Fish eye sockets have high oil content, especially sea fish fish eye sockets contain a large amount of polyunsaturated fatty acids, among which ω-3 highly unsaturated fatty acids (mainly DHA, EPA) have special physiological effects: prevention and treatment of cardiovascular diseases, anti-inflammation , anti-cancer, enhancing immune function and promoting the growth and development of young animals; especially DHA can promote the growth of brain development, intelligence and memory, etc. It is known as "brain gold" and "the driving force of intelligence", but the human body Can not synthesize DHA and EPA by itself, can only be ingested from food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: a kind of preparation method of fish eye socket active oil, comprises the following steps successively:

[0026] 1. Fresh (or cryopreserved, thawed in running water) fish head, remove the skin and bones, obtain the eye socket meat (the meat around the eyes), and homogenate.

[0027] 2. Ultrasound-assisted compound enzyme enzymolysis: add 0.5 times the volume of water to the homogenate; process the homogenate with 300 watts of ultrasonic waves for 10 minutes; adjust the pH to 6 with 1 mol / L hydrochloric acid; add neutral protease ( EC 3.4.23.6), the amount of enzyme added is 0.1% of the weight of fish brain homogenate, enzymolysis at 30°C for 1 hour; 100°C for 10 minutes to inactivate the enzyme; adjust the pH to 7 with 1 mol / L sodium hydroxide; add alkaline protease (EC 3.4.21.14), the amount of enzyme added is 0.1% of the weight of fish brain homogenate, enzymolysis at 30°C for 1 hour; after enzymolysis, inactivate the enzyme at 100°C for 10 minutes, and ...

Embodiment 2

[0030] Embodiment 2: a kind of preparation method of fish eye socket active oil, comprises the following steps successively:

[0031] 1. Fresh (or cryopreserved, thawed in running water) fish head, remove the skin and bones, obtain the eye socket meat (the meat around the eyes), and homogenate.

[0032] 2. Ultrasound-assisted compound enzyme enzymolysis: add 1.5 times the volume of water to the homogenate; use 800 watts of ultrasonic waves to treat the homogenate for 30 minutes; adjust the pH to 9 with 3 mol / L sodium hydroxide; add neutral Protease (EC 3.4.23.6), the amount of enzyme added is 2% of the weight of fish brain homogenate, enzymolysis at 60°C for 3 hours; enzyme inactivation at 90°C for 15 minutes; adjust the pH to 11 with 3 mol / L sodium hydroxide; add alkali Protease (EC 3.4.21.14), the amount of enzyme added is 2% of the weight of fish brain homogenate, enzymolysis at 60°C for 3 hours; after enzymolysis, inactivate the enzyme at 90°C for 15 minutes, and centrifug...

Embodiment 3

[0035] Embodiment 3: a kind of preparation method of fish eye socket active oil, comprises the following steps successively:

[0036] 1. Fresh (or cryopreserved, thawed in running water) fish head, remove the skin and bones, obtain the eye socket meat (the meat around the eyes), and homogenate.

[0037] 2. Ultrasound-assisted compound enzyme enzymolysis: add 1 times the volume of water to the homogenate; process the homogenate with 400 watts of ultrasonic waves for 20 minutes; adjust the pH to 7 with 3 mol / L hydrochloric acid; add neutral protease ( EC3.4.23.6), the amount of enzyme added is 0.5% of the weight of fish brain homogenate, enzymolysis at 40°C for 2 hours; 100°C to inactivate the enzyme for 10 minutes; adjust the pH to 8 with 3 mol / L sodium hydroxide; add alkaline Protease (EC 3.4.21.14), the amount of enzyme added is 0.5% of the weight of fish brain homogenate, enzymolysis at 40°C for 2 hours; after enzymolysis, inactivate the enzyme at 95°C for 10 minutes, centri...

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Abstract

The invention discloses a preparation method for fish eye socket active grease. The preparation method for the fish eye socket active grease includes the steps: removing skins and bones of fresh fish heads to get eye socket meat and homogenizing; carrying out ultrasonication; adding neutral protease to carry our enzymolysis; adding alkaline protease to carry out the enzymolysis after deactivating enzyme; achieving enzymatic hydrolysate after deactivating enzyme; achieving free oil, an emulsus layer, a water layer and a precipitate layer after centrifuging; merging the emulsus layer and the water layer, and extracting grease through a supercritical carbon dioxide extraction method; and merging the achieved grease to get the fish eye socket active grease. According to the preparation method for the fish eye socket active grease, waste fish heads of traditional fishery are adopted as raw materials for preparing active grease products, so that the preparation method for the fish eye socket active grease has significance in fish comprehensive processing and utilization and environment protection, and economical benefit is improved. Ultrasonic is adopted to assist the complex enzyme for the enzymolysis of fish sockets, no organic solvent is used in the process of extraction, and the preparing method is environmental-friendly and high in a recycle rate of the grease. The preparation method for the fish eye socket active grease is simple and convenient to operate, moderate in conditions of the extraction method, and capable of preserving certain original physiological activators in the eye socket grease to a maximum degree and enabling the products to have trophism and functionality.

Description

technical field [0001] The invention relates to a method for extracting fish eye socket substances, in particular to a method for extracting active oil by using fish eye sockets. Background technique [0002] Since the 1950s, the world's total fishery output has been increasing at a rate of 6%-7%. Although the fishery output has already exceeded 100 million tons, nearly 1 / 3 of the catch has not been directly utilized by humans. The total amount of aquatic products in my country has exceeded 52 million tons, and it has become the world's largest fishery production country for 20 consecutive years. Fish heads are the main leftovers in the fish processing process, accounting for about 15-30% of the total weight of fish. Fish head is rich in protein, fat, calcium, phosphorus, iron, and vitamins. The amino acid pattern of protein is close to the needs of the human body. The fatty acid is rich in unsaturated fatty acids such as DHA and EPA and phospholipids, which has certain dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/02
Inventor 朱蓓薇沈琳秦磊孙黎明董秀萍雷康陶恒亮姜鹏飞
Owner DALIAN POLYTECHNIC UNIVERSITY
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