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Folium orthosiphoni effective part for treating diabetic nephropathy and preparation method

A technology for diabetic nephropathy and effective parts, which is applied in the field of medicine to achieve obvious effects, simple treatment process and wide market prospects

Inactive Publication Date: 2013-06-05
中国医学科学院药用植物研究所云南分所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dai medicine Zhongshen tea is a kind of antidote commonly used in Dai medicine. It is recorded in the scriptures that it can be used as a decoction or soaked in boiling water as a tea drink. Modern studies have shown that the flavonoids in kidney tea have significant diuretic, anti-inflammatory, anti-oxidant, and free radical scavenging effects. No.: 2011101859979), on this basis, the research group conducted in-depth research and found that the total flavonoids in kidney tea include aurantrinone, perrinin, and 3'-hydroxy-5,6,7,4'-tetramethoxyflavone , has significant activity in treating diabetic nephropathy, and its mechanism of action may be that the effective parts of kidney tea combine with hypoglycemic and anti-nephritis effects. At present, there is no report on the effect of effective parts of kidney tea in treating diabetic nephropathy. The present invention aims at the effective parts of kidney tea in treating diabetic nephropathy. Research and development, with broad market prospects

Method used

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  • Folium orthosiphoni effective part for treating diabetic nephropathy and preparation method
  • Folium orthosiphoni effective part for treating diabetic nephropathy and preparation method
  • Folium orthosiphoni effective part for treating diabetic nephropathy and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh the kidney tea medicinal material, add 5 times of 30% ethanol for reflux extraction for 1 hour, filter, add 5 times of 30% ethanol to the filter residue and rereflux for 1 hour, combine the two filtrates, concentrate in a rotary evaporator, and concentrate to The extract containing 0.8g / mL crude drug. Take the extract prepared above, fully mix and absorb with 5 times the volume of macroporous resin, put the mixture on the column, pack it firmly, elute with deionized water until colorless, then elute with 30% ethanol until colorless, and use 30% ethanol to elute until colorless. % ethanol for elution until colorless, the eluate was collected, concentrated into an extract in a rotary evaporator, and dried under reduced pressure to obtain the effective fraction of Kidney Tea, the total flavonoid content was determined to be 51.3%.

Embodiment 2

[0017] Weigh the medicinal material of kidney tea, add 15 times of 80% ethanol for reflux extraction for 2 hours, filter, add 15 times of 80% ethanol to the filter residue and rereflux for 2 hours, combine the two filtrates, concentrate in a rotary evaporator, and concentrate to The extract containing 1.3g / mL crude drug. Take the extract prepared above, fully mix and absorb with 15 times the volume of macroporous resin, put the mixture on the column, pack it firmly, elute it with deionized water until it is colorless, and then elute it with 30% ethanol until it is colorless, and use 60 % ethanol for elution until colorless, the eluate was collected, concentrated in a rotary evaporator to an extract, dried under reduced pressure to obtain the effective fraction of Kidney Tea, and the total flavonoid content was determined to be 59.2%.

Embodiment 3

[0019] Weigh the medicinal material of kidney tea, add 10 times of 60% ethanol for reflux extraction for 1.5 hours, filter, add 10 times of 60% ethanol to the filter residue and rereflux for 1.5 hours, combine the two filtrates, concentrate in a rotary evaporator, and concentrate to The extract containing 1.1g / mL crude drug. Take the above-mentioned prepared extract, fully mix and absorb with 10 times the volume of macroporous resin, put the mixture on the column, pack it firmly, elute with deionized water until it is colorless, and then elute it with 30% ethanol until it is colorless, and use 50 % ethanol for elution until colorless, the eluate was collected, concentrated into an extract in a rotary evaporator, and dried under reduced pressure to obtain the effective fraction of Kidney Tea. The total flavonoid content was determined to be 65.7%.

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Abstract

The invention discloses a folium orthosiphoni effective part for treating diabetic nephropathy and a preparation method, which comprises the following steps: 1) extracting folium orthosiphoni and concentrating; 2) applying a folium orthosiphoni extract onto column; and 3) eluting, collecting and concentrating folium orthosiphoni effective part. The folium orthosiphoni effective part is mixed with current drug and a food excipient for preparing drugs, food and health care products for treating diabetic nephropathy. The extracted effective part has high content, the content of general flavone can reach more than 50%, and reach five types of traditional Chinese medicine materials standard, an eluent has no toxicity, and the preparation method is suitable for industrial production.

Description

technical field [0001] The invention relates to the application of effective parts of traditional Chinese medicine and its preparation method, specifically refers to the use of macroporous adsorption resin to enrich the effective parts of kidney tea for treating diabetic nephropathy, which are used in medicine, food and health care products for treating diabetic nephropathy, belonging to medicine technology field. Background technique [0002] Diabetic nephropathy belongs to the category of diabetic microangiopathy and is a common clinical secondary kidney disease. According to statistics, diabetic nephropathy accounts for 60% of the mortality rate of diabetic patients, and the death of diabetic nephropathy and renal failure is 17 times that of non-diabetic patients. Diabetic nephropathy has become the main cause of end-stage renal disease (ESRD) in western countries, and has become an important factor affecting the quality of life and mortality of diabetic patients. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P3/10A61P13/12A23L1/29A23L33/00
Inventor 陈曦李光赵俊凌路娟李学兰马小军
Owner 中国医学科学院药用植物研究所云南分所
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