Method for building animal model with hyperuricemia-combined diabetes

Abstract

The invention discloses a method for building an animal model with hyperuricemia-combined diabetes. The method comprises the following steps: 1) feeding an target animal with fodder containing 10% (by mass) of yeast powder; subjecting the target animal to a lavage with a 4% (by mass) adenine water solution according to the following dosage of 100 milligram of adenine per day per kilogram of body weight until the blood uric acid level of the target animal starts falling; 2) halving the dosage of the adenine water solution in step 1), subjecting the target animal to a subcutaneous injection with an oteracil potassium emulsion twice a day according to the following dosage of 100 milligram of oteracil potassium emulsion per day per kilogram of body weight until the target animal shows clinical symptoms of diabetes, and then obtaining the animal model with the hyperuricemia-combined diabetes. The method builds the animal model with the hyperuricemia-combined diabetes for the first time, the animal model has typical disease symptoms, the quantity index has statistical significance, and studies such as glucose clamp test and immunohistochemistry also prove that the modeling is successful. Therefore, the method for building the animal model with the hyperuricemia-combined diabetes is a reliable and practical technique.

Description

technical field [0001] The invention relates to a method for constructing an animal model of hyperuricemia combined with diabetes. Background technique [0002] In recent years, with the improvement of living standards and the adjustment of dietary structure, the incidence of hyperuricemia combined with diabetes has been increasing year by year. A large number of studies have shown that the rate of diabetic patients with hyperuricemia is significantly higher than that of non-diabetic patients. 20%-50% of patients with hyperuricemia have diabetes at the same time, and in elderly patients with type 2 diabetes, hyperuricemia The incidence rate is about 35%. Clinical studies have shown that, as an independent risk factor for atherosclerosis and coronary heart disease, hyperuricemia also has a significant impact on chronic complications of diabetes: BoS et al. reported that hyperuricemia can accelerate the occurrence and development of diabetic nephropathy in patients with type ...

AI Technical Summary

Problems solved by technology

Although the blood sugar of this model is obviously elevated, the uric acid is not high. In addition, although the clinical manifestations of this model are similar to those of type 2 diabetes, the mechanism leading to the increase of blood sugar is obviously different from that of type 2 diabetes, so it cannot be used as a model for studying the pathogenesis of type 2 diabetes. Model

Images